Effect of ocean acidification and pH fluctuations on the growth and development of coralline algal recruits, and an associated benthic algal assemblage, supplement to: Roleda, Michael Y; Cornwall, Christopher Edward; Feng, Yuanyuan; McGraw, Christina M; Smith, Abigail M; Hurd, Catriona L (2015): Effect of ocean acidification and pH fluctuations on the growth and development of coralline algal recruits, and an associated benthic algal assemblage. PLoS ONE, 10(10), e0140394

• Main Authors: Roleda, Michael Y, Cornwall, Christopher Edward, Feng, Yuanyuan, McGraw, Christina M, Smith, Abigail M, Hurd, Catriona L
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2015
• Subjects: Arthrocardia corymbosa; Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Macroalgae; Plantae; Rhodophyta; Single species; South Pacific; Temperate; Type; Species; Registration number of species; Uniform resource locator/link to reference; Treatment; Date; Area; Area, standard error; Growth rate; Growth rate, standard error; Number; Number, standard error; Magnesium carbonate, magnesite; Magnesium carbonate, magnesite, standard error; Chlorophyll a/particulate organic carbon ratio; Chlorophyll a/particulate organic carbon ratio, standard error; Biogenic silica per chlorophyll a; Biogenic silica per chlorophyll a, standard error; Salinity; Temperature, water; pH; pH, standard error; Alkalinity, total; Alkalinity, total, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Experiment; Potentiometric; Potentiometric titration; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Cornwall; Hurd; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.859434; https://doi.pangaea.de/10.1594/PANGAEA.859434

Coralline algae are susceptible to the changes in the seawater carbonate system associated with ocean acidification (OA). However, the coastal environments in which corallines grow are subject to large daily pH fluctuations which may affect their responses to OA. Here, we followed the growth and development of the juvenile coralline alga Arthrocardia corymbosa, which had recruited into experimental conditions during a prior experiment, using a novel OA laboratory culture system to simulate the pH fluctuations observed within a kelp forest. Microscopic life history stages are considered more susceptible to environmental stress than adult stages; we compared the responses of newly recruited A. corymbosa to static and fluctuating seawater pH with those of their field-collected parents. Recruits were cultivated for 16 weeks under static pH 8.05 and 7.65, representing ambient and 4*preindustrial pCO2 concentrations, respectively, and two fluctuating pH treatments of daily (daytime pH = 8.45, night-time pH = 7.65) and daily (daytime pH = 8.05, night-time pH = 7.25). Positive growth rates of new recruits were recorded in all treatments, and were highest under static pH 8.05 and lowest under fluctuating pH 7.65. This pattern was similar to the adults' response, except that adults had zero growth under fluctuating pH 7.65. The % dry weight of MgCO3 in calcite of the juveniles was reduced from 10% at pH 8.05 to 8% at pH 7.65, but there was no effect of pH fluctuation. A wide range of fleshy macroalgae and at least 6 species of benthic diatoms recruited across all experimental treatments, from cryptic spores associated with the adult A. corymbosa. There was no effect of experimental treatment on the growth of the benthic diatoms. On the community level, pH-sensitive species may survive lower pH in the presence of diatoms and fleshy macroalgae, whose high metabolic activity may raise the pH of the local microhabitat. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2016-04-08.