Nitrate limitation and ocean acidification interact with UV-B to reduce photosynthetic performance in the diatom ...

• Main Authors: Li, Wei, Gao, Kunshan, Beardall, John
• Format: Dataset
• Language: English
• Published: PANGAEA 2015
• Subjects: Bottles or small containers/Aquaria <20 L; Charophyta; Chromista; Laboratory experiment; Laboratory strains; Light; Macro-nutrients; North Atlantic; Ochrophyta; Pelagos; Phaeodactylum tricornutum; Phytoplankton; Primary production/Photosynthesis; Single species; Species; Figure; Table; Treatment; Photosynthetic carbon fixation rate, per cell; Photosynthetic carbon fixation rate, standard deviation; Photosynthetic carbon fixation rate, per chlorophyll a; Ultraviolet-a radiation-induced inhibition of carbon fixation; Ultraviolet-a radiation-induced inhibition of carbon fixation, standard deviation; Ultraviolet-b radiation-induced inhibition of carbon fixation; Ultraviolet-b radiation-induced inhibition of carbon fixation, standard deviation; Ultraviolet-a radiation-induced inhibition of effective photochemical quantum yield; Ultraviolet-a radiation-induced inhibition of effective photochemical quantum yield, standard deviation; Ultraviolet-b radiation-induced inhibition of effective photochemical quantum yield; Ultraviolet-b radiation-induced inhibition of effective photochemical quantum yield, standard deviation; Time in minutes; Non photochemical quenching; Non photochemical quenching, standard deviation; Protein per cell; Proteins, standard deviation; Superoxide dismutase activity, unit per protein mass; Superoxide dismutase activity, standard deviation; Superoxide dismutase activity, unit per cell; Catalase activity, unit per protein mass; Catalase activity, standard deviation; Catalase activity, unit per cell; Effective quantum yield; Effective quantum yield, standard deviation; Time, standard deviation; Exponential rate constant for recovery; Exponential rate constant for recovery, standard deviation; Repair rate; Repair rate, standard deviation; Damage rate; Damage rate, standard deviation; Damage/repair ratio; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.851339; https://doi.pangaea.de/10.1594/PANGAEA.851339

It has been proposed that ocean acidification (OA) will interact with other environmental factors to influence the overall impact of global change on biological systems. Accordingly we investigated the influence of nitrogen limitation and OA on the physiology of diatoms by growing the diatom Phaeodactylum tricornutum Bohlin under elevated (1000 µatm; high CO2- HC) or ambient (390 µatm; low CO2-LC) levels of CO2 with replete (110 µmol/L; high nitrate-HN) or reduced (10 ?mol/L; low nitrate-LN) levels of NO3- and subjecting the cells to solar radiation with or without UV irradiance to determine their susceptibility to UV radiation (UVR, 280-400 nm). Our results indicate that OA and UVB induced significantly higher inhibition of both the photosynthetic rate and quantum yield under LN than under HN conditions. UVA or/and UVB increased the cells' non-photochemical quenching (NPQ) regardless of the CO2 levels. Under LN and OA conditions, activity of superoxide dismutase and catalase activities were enhanced, along ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-20. ...