The effect of elevated CO2 and increased temperature on in vitro fertilization success and initial embryonic development of single male:female crosses of broad-cast spawning corals at mid- and high-latitude locations, supplement to: Schutter, Miriam; Nozawa, Yoko; Kurihara, Haruko (2015): The effect of elevated CO2 and increased temperature on in vitro fertilization success and initial embryonic development of single male:female crosses of broad-cast spawning corals at mid- and high-latitude locations. Journal of Marine Science and Engineering, 3(2), 216-239

• Main Authors: Schutter, Miriam, Nozawa, Yoko, Kurihara, Haruko
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2015
• Subjects: Acropora hyacinthus; Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Cnidaria; Coast and continental shelf; Development; Favites abdita; Laboratory experiment; North Pacific; Platygyra contorta; Reproduction; FOS Medical biotechnology; Single species; Temperate; Temperature; Tropical; Event label; Species; Treatment; Experiment; Temperature, water; Temperature, water, standard deviation; pH; pH, standard deviation; Salinity; Alkalinity, total; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Fertilization success rate; Abnormality; Early developmental success embryos/eggs+embryos; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.849254; https://doi.pangaea.de/10.1594/PANGAEA.849254

The impact of global climate change on coral reefs is expected to be most profound at the sea surface, where fertilization and embryonic development of broadcast-spawning corals takes place. We examined the effect of increased temperature and elevated CO2 levels on the in vitro fertilization success and initial embryonic development of broadcast-spawning corals using a single male:female cross of three different species from mid- and high-latitude locations: Lyudao, Taiwan (22° N) and Kochi, Japan (32° N). Eggs were fertilized under ambient conditions (27 °C and 500 µatm CO2) and under conditions predicted for 2100 (IPCC worst case scenario, 31 °C and 1000 µatm CO2). Fertilization success, abnormal development and early developmental success were determined for each sample. Increased temperature had a more profound influence than elevated CO2. In most cases, near-future warming caused a significant drop in early developmental success as a result of decreased fertilization success and/or increased abnormal development. The embryonic development of the male:female cross of A. hyacinthus from the high-latitude location was more sensitive to the increased temperature (+4 °C) than the male:female cross of A. hyacinthus from the mid-latitude location. The response to the elevated CO2 level was small and highly variable, ranging from positive to negative responses. These results suggest that global warming is a more significant and universal stressor than ocean acidification on the early embryonic development of corals from mid- and high-latitude locations. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-09-09.