Enzyme activity, body measures and heart mass of Sepia officinalis sampled in the English Channel and Adriatic Sea ...

• Main Authors: Oellermann, Michael, Pörtner, Hans-Otto, Mark, Felix Christopher
• Format: Article in Journal/Newspaper
• Language: English
• Published: PANGAEA 2011
• Subjects: Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158; Arctic; Antarctic; Antarc*; Sea ice
• Online Access: https://dx.doi.org/10.1594/pangaea.848989; https://doi.pangaea.de/10.1594/PANGAEA.848989

In the eurythermal cuttlefish Sepia officinalis, performance depends on hearts that ensure systemic oxygen supply over a broad range of temperatures. We therefore aimed to identify adjustments in energetic cardiac capacity and underlying mitochondrial function supporting thermal acclimation and adaptation that could be crucial for the cuttlefish's competitive success in variable environments. Two genetically distinct cuttlefish populations were acclimated to 11, 16 and 21°C. Subsequently, skinned and permeabilised heart fibres were used to assess mitochondrial functioning by means of high-resolution respirometry and a substrate-inhibitor protocol, followed by measurements of cardiac citrate synthase and cytosolic enzyme activities. Temperate English Channel cuttlefish had lower mitochondrial capacities but larger hearts than subtropical Adriatic cuttlefish. Warm acclimation to 21°C decreased mitochondrial complex I activity in Adriatic cuttlefish and increased complex IV activity in English Channel ... : Supplement to: Oellermann, Michael; Pörtner, Hans-Otto; Mark, Felix Christopher (2012): Mitochondrial dynamics underlying thermal plasticity of cuttlefish (Sepia officinalis) hearts. Journal of Experimental Biology, 215(17), 2992-3000 ...