Lipid, carbon and nitrogen content and fatty acid composition of Temora longicornis females and different food species during the experiment ...

• Main Authors: Kreibich, Tobias, Saborowski, Reinhard, Hagen, Wilhelm, Niehoff, Barbara
• Format: Article in Journal/Newspaper
• Language: English
• Published: PANGAEA 2011
• Subjects: AWI; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas SPP1158; Antarc*; Antarctic; Sea ice
• Online Access: https://dx.doi.org/10.1594/pangaea.848501; https://doi.pangaea.de/10.1594/PANGAEA.848501

Temora longicornis, a dominant calanoid copepod species in the North Sea, is characterised by low lipid reserves and high biomass turnover rates. To survive and reproduce successfully, this species needs continuous food supply and thus requires a highly flexible digestive system to exploit various food sources. Information on the capacity of digestive enzymes is scarce and therefore the aim of our study was to investigate the enzymatic capability to respond to quickly changing nutritional conditions. We conducted two feeding experiments with female T. longicornis from the southern North Sea off Helgoland. In the first experiment in 2005, we tested how digestive enzyme activities and enzyme patterns as revealed by substrate SDS-PAGE (sodium dodecylsulfate polyacrylamide gel electrophoresis) responded to changes in food composition. Females were incubated for three days fed ad libitum with either the heterotrophic dinoflagellate Oxyrrhis marina or the diatom Thalassiosira weissflogii. At the beginning and at ... : Supplement to: Kreibich, Tobias; Saborowski, Reinhard; Hagen, Wilhelm; Niehoff, Barbara (2011): Influence of short-term nutritional variations on digestive enzyme and fatty acid patterns of the calanoid copepod Temora longicornis. Journal of Experimental Marine Biology and Ecology, 407(2), 182-189 ...