Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes, supplement to: Moya, Aurélie; Huisman, L; Forêt, S; Gattuso, Jean-Pierre; Hayward, D C; Ball, E E; Miller, David J (2015): Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes. Molecular Ecology, 24(2), 438-452
Corals play a key role in ocean ecosystems and carbonate balance, but their molecular response to ocean acidification remains unclear. The only previous whole-transcriptome study documented extensive disruption of gene expression, particularly of genes encoding skeletal organic matrix proteins, in j...
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Format: | Dataset |
Language: | English |
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PANGAEA - Data Publisher for Earth & Environmental Science
2015
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Online Access: | https://dx.doi.org/10.1594/pangaea.847720 https://doi.pangaea.de/10.1594/PANGAEA.847720 |
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ftdatacite:10.1594/pangaea.847720 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Acropora millepora Animalia Benthic animals Benthos Cnidaria Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Gene expression incl. proteomics Laboratory experiment Single species South Pacific Tropical Species Figure Treatment Gene abundance Group Percentage Family Number of sequences pH pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Temperature, water Temperature, water, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Salinity Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Calcite saturation state Potentiometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Acropora millepora Animalia Benthic animals Benthos Cnidaria Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Gene expression incl. proteomics Laboratory experiment Single species South Pacific Tropical Species Figure Treatment Gene abundance Group Percentage Family Number of sequences pH pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Temperature, water Temperature, water, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Salinity Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Calcite saturation state Potentiometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Moya, Aurélie Huisman, L Forêt, S Gattuso, Jean-Pierre Hayward, D C Ball, E E Miller, David J Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes, supplement to: Moya, Aurélie; Huisman, L; Forêt, S; Gattuso, Jean-Pierre; Hayward, D C; Ball, E E; Miller, David J (2015): Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes. Molecular Ecology, 24(2), 438-452 |
topic_facet |
Acropora millepora Animalia Benthic animals Benthos Cnidaria Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Gene expression incl. proteomics Laboratory experiment Single species South Pacific Tropical Species Figure Treatment Gene abundance Group Percentage Family Number of sequences pH pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Temperature, water Temperature, water, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Salinity Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Calcite saturation state Potentiometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
description |
Corals play a key role in ocean ecosystems and carbonate balance, but their molecular response to ocean acidification remains unclear. The only previous whole-transcriptome study documented extensive disruption of gene expression, particularly of genes encoding skeletal organic matrix proteins, in juvenile corals (Acropora millepora) after short-term (3 d) exposure to elevated pCO2. In this study, whole-transcriptome analysis was used to compare the effects of such 'acute' (3 d) exposure to elevated pCO2 with a longer ('prolonged'; 9 d) period of exposure beginning immediately post-fertilization. Far fewer genes were differentially expressed under the 9-d treatment, and although the transcriptome data implied wholesale disruption of metabolism and calcification genes in the acute treatment experiment, expression of most genes was at control levels after prolonged treatment. There was little overlap between the genes responding to the acute and prolonged treatments, but heat shock proteins (HSPs) and heat shock factors (HSFs) were over-represented amongst the genes responding to both treatments. Amongst these was an HSP70 gene previously shown to be involved in acclimation to thermal stress in a field population of another acroporid coral. The most obvious feature of the molecular response in the 9-d treatment experiment was the upregulation of five distinct Bcl-2 family members, the majority predicted to be anti-apoptotic. This suggests that an important component of the longer term response to elevated CO2 is suppression of apoptosis. It therefore appears that juvenile A. millepora have the capacity to rapidly acclimate to elevated pCO2, a process mediated by upregulation of specific HSPs and a suite of Bcl-2 family members. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-07-03. |
format |
Dataset |
author |
Moya, Aurélie Huisman, L Forêt, S Gattuso, Jean-Pierre Hayward, D C Ball, E E Miller, David J |
author_facet |
Moya, Aurélie Huisman, L Forêt, S Gattuso, Jean-Pierre Hayward, D C Ball, E E Miller, David J |
author_sort |
Moya, Aurélie |
title |
Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes, supplement to: Moya, Aurélie; Huisman, L; Forêt, S; Gattuso, Jean-Pierre; Hayward, D C; Ball, E E; Miller, David J (2015): Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes. Molecular Ecology, 24(2), 438-452 |
title_short |
Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes, supplement to: Moya, Aurélie; Huisman, L; Forêt, S; Gattuso, Jean-Pierre; Hayward, D C; Ball, E E; Miller, David J (2015): Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes. Molecular Ecology, 24(2), 438-452 |
title_full |
Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes, supplement to: Moya, Aurélie; Huisman, L; Forêt, S; Gattuso, Jean-Pierre; Hayward, D C; Ball, E E; Miller, David J (2015): Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes. Molecular Ecology, 24(2), 438-452 |
title_fullStr |
Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes, supplement to: Moya, Aurélie; Huisman, L; Forêt, S; Gattuso, Jean-Pierre; Hayward, D C; Ball, E E; Miller, David J (2015): Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes. Molecular Ecology, 24(2), 438-452 |
title_full_unstemmed |
Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes, supplement to: Moya, Aurélie; Huisman, L; Forêt, S; Gattuso, Jean-Pierre; Hayward, D C; Ball, E E; Miller, David J (2015): Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes. Molecular Ecology, 24(2), 438-452 |
title_sort |
rapid acclimation of juvenile corals to co2-mediated acidification by upregulation of heat shock protein and bcl-2 genes, supplement to: moya, aurélie; huisman, l; forêt, s; gattuso, jean-pierre; hayward, d c; ball, e e; miller, david j (2015): rapid acclimation of juvenile corals to co2-mediated acidification by upregulation of heat shock protein and bcl-2 genes. molecular ecology, 24(2), 438-452 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2015 |
url |
https://dx.doi.org/10.1594/pangaea.847720 https://doi.pangaea.de/10.1594/PANGAEA.847720 |
long_lat |
ENVELOPE(167.350,167.350,-78.117,-78.117) |
geographic |
Hayward Pacific |
geographic_facet |
Hayward Pacific |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1111/mec.13021 https://cran.r-project.org/package=seacarb |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.847720 https://doi.org/10.1111/mec.13021 |
_version_ |
1766158020906057728 |
spelling |
ftdatacite:10.1594/pangaea.847720 2023-05-15T17:51:02+02:00 Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes, supplement to: Moya, Aurélie; Huisman, L; Forêt, S; Gattuso, Jean-Pierre; Hayward, D C; Ball, E E; Miller, David J (2015): Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes. Molecular Ecology, 24(2), 438-452 Moya, Aurélie Huisman, L Forêt, S Gattuso, Jean-Pierre Hayward, D C Ball, E E Miller, David J 2015 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.847720 https://doi.pangaea.de/10.1594/PANGAEA.847720 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1111/mec.13021 https://cran.r-project.org/package=seacarb Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Acropora millepora Animalia Benthic animals Benthos Cnidaria Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Gene expression incl. proteomics Laboratory experiment Single species South Pacific Tropical Species Figure Treatment Gene abundance Group Percentage Family Number of sequences pH pH, standard deviation Alkalinity, total Alkalinity, total, standard deviation Temperature, water Temperature, water, standard deviation Aragonite saturation state Aragonite saturation state, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Salinity Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Calcite saturation state Potentiometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Supplementary Dataset dataset Dataset 2015 ftdatacite https://doi.org/10.1594/pangaea.847720 https://doi.org/10.1111/mec.13021 2021-11-05T12:55:41Z Corals play a key role in ocean ecosystems and carbonate balance, but their molecular response to ocean acidification remains unclear. The only previous whole-transcriptome study documented extensive disruption of gene expression, particularly of genes encoding skeletal organic matrix proteins, in juvenile corals (Acropora millepora) after short-term (3 d) exposure to elevated pCO2. In this study, whole-transcriptome analysis was used to compare the effects of such 'acute' (3 d) exposure to elevated pCO2 with a longer ('prolonged'; 9 d) period of exposure beginning immediately post-fertilization. Far fewer genes were differentially expressed under the 9-d treatment, and although the transcriptome data implied wholesale disruption of metabolism and calcification genes in the acute treatment experiment, expression of most genes was at control levels after prolonged treatment. There was little overlap between the genes responding to the acute and prolonged treatments, but heat shock proteins (HSPs) and heat shock factors (HSFs) were over-represented amongst the genes responding to both treatments. Amongst these was an HSP70 gene previously shown to be involved in acclimation to thermal stress in a field population of another acroporid coral. The most obvious feature of the molecular response in the 9-d treatment experiment was the upregulation of five distinct Bcl-2 family members, the majority predicted to be anti-apoptotic. This suggests that an important component of the longer term response to elevated CO2 is suppression of apoptosis. It therefore appears that juvenile A. millepora have the capacity to rapidly acclimate to elevated pCO2, a process mediated by upregulation of specific HSPs and a suite of Bcl-2 family members. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-07-03. Dataset Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) Hayward ENVELOPE(167.350,167.350,-78.117,-78.117) Pacific |