Effects of CO2 and iron availability on rbcL gene expression in Bering Sea diatoms ...

• Main Authors: Endo, H, Sugie, Koji, Yoshimura, T, Suzuki, Koji
• Format: Dataset
• Language: English
• Published: PANGAEA 2015
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Entire community; Gene expression incl. proteomics; Laboratory experiment; Micro-nutrients; North Pacific; Open ocean; Pelagos; Temperate; Treatment; Day of experiment; Fucoxanthin; Fucoxanthin, standard deviation; 19-Hexanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin, standard deviation; Class; Contribution; Gene copies; Gene copies, standard deviation; Deoxyribonucleic acid, complementary; Deoxyribonucleic acid, complementary, standard deviation; Family; Temperature, water; Salinity; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; pH; pH, standard deviation; Nitrate; Nitrate, standard deviation; Phosphate; Phosphate, standard deviation; Silicate; Silicate, standard deviation; Iron, dissolved; Iron, dissolved, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Experiment; Bering Sea; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.847229; https://doi.pangaea.de/10.1594/PANGAEA.847229

Iron (Fe) can limit phytoplankton productivity in approximately 40% of the global ocean, including in high-nutrient, low-chlorophyll (HNLC) waters. However, there is little information available on the impact of CO2-induced seawater acidification on natural phytoplankton assemblages in HNLC regions. We therefore conducted an on-deck experiment manipulating CO2 and Fe using Fe-deficient Bering Sea water during the summer of 2009. The concentrations of CO2 in the incubation bottles were set at 380 and 600 ppm in the non-Fe-added (control) bottles and 180, 380, 600, and 1000 ppm in the Fe-added bottles. The phytoplankton assemblages were primarily composed of diatoms followed by haptophytes in all incubation bottles as estimated by pigment signatures throughout the 5-day (control) or 6-day (Fe-added treatment) incubation period. At the end of incubation, the relative contribution of diatoms to chlorophyll a biomass was significantly higher in the 380 ppm CO2 treatment than in the 600 ppm treatment in the ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-06-01. ...