Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2 ...

• Main Authors: Wei, Lei, Wang, Qing, Ning, Xuanxuan, Mu, Changkao, Wang, Chunlin, Cao, Ruiwen, Wu, Huifeng, Cong, Ming, Li, Fei, Ji, Chenglong, Zhao, Jianmin
• Format: Dataset
• Language: English
• Published: PANGAEA 2015
• Subjects: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Crassostrea gigas; Gene expression incl. proteomics; Laboratory experiment; Mollusca; Single species; South Pacific; Temperate; Species; Treatment; Gene name; mRNA gene expression, relative; mRNA gene expression, relative, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Salinity, standard deviation; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification; Pacific oyster
• Online Access: https://dx.doi.org/10.1594/pangaea.846883; https://doi.pangaea.de/10.1594/PANGAEA.846883

Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO2 condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO2 could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO2 probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C. gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels. ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-06-01. ...