Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2, supplement to: Schiffer, Melanie; Harms, Lars; Lucassen, Magnus; Mark, Felix Christopher; Pörtner, Hans-Otto; Storch, Daniela (2014): Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2. Frontiers in Zoology, 11(1)
Exposure to elevated seawater PCO2 limits the thermal tolerance of crustaceans but the underlying mechanisms have not been comprehensively explored. Larval stages of crustaceans are even more sensitive to environmental hypercapnia and possess narrower thermal windows than adults. In a mechanistic ap...
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Format: | Dataset |
Language: | English |
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PANGAEA - Data Publisher for Earth & Environmental Science
2014
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Online Access: | https://dx.doi.org/10.1594/pangaea.846762 https://doi.pangaea.de/10.1594/PANGAEA.846762 |
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ftdatacite:10.1594/pangaea.846762 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Animalia Arthropoda Behaviour Bottles or small containers/Aquaria <20 L Coast and continental shelf Gene expression incl. proteomics Hyas araneus Laboratory experiment Mortality/Survival North Atlantic Pelagos Respiration Single species Temperate Temperature Zooplankton Species Table Figure Day of experiment Temperature, water Treatment Stage Oxygen consumption Heart beat rate Heart rate Mortality Gene name Gene expression Standard deviation Temperature, water, standard deviation pH pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Salinity Salinity, standard deviation Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Calculated using seacarb after Nisumaa et al. 2010 Biological Impacts of Ocean Acidification BIOACID Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Animalia Arthropoda Behaviour Bottles or small containers/Aquaria <20 L Coast and continental shelf Gene expression incl. proteomics Hyas araneus Laboratory experiment Mortality/Survival North Atlantic Pelagos Respiration Single species Temperate Temperature Zooplankton Species Table Figure Day of experiment Temperature, water Treatment Stage Oxygen consumption Heart beat rate Heart rate Mortality Gene name Gene expression Standard deviation Temperature, water, standard deviation pH pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Salinity Salinity, standard deviation Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Calculated using seacarb after Nisumaa et al. 2010 Biological Impacts of Ocean Acidification BIOACID Ocean Acidification International Coordination Centre OA-ICC Schiffer, Melanie Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2, supplement to: Schiffer, Melanie; Harms, Lars; Lucassen, Magnus; Mark, Felix Christopher; Pörtner, Hans-Otto; Storch, Daniela (2014): Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2. Frontiers in Zoology, 11(1) |
topic_facet |
Animalia Arthropoda Behaviour Bottles or small containers/Aquaria <20 L Coast and continental shelf Gene expression incl. proteomics Hyas araneus Laboratory experiment Mortality/Survival North Atlantic Pelagos Respiration Single species Temperate Temperature Zooplankton Species Table Figure Day of experiment Temperature, water Treatment Stage Oxygen consumption Heart beat rate Heart rate Mortality Gene name Gene expression Standard deviation Temperature, water, standard deviation pH pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Salinity Salinity, standard deviation Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Calculated using seacarb after Nisumaa et al. 2010 Biological Impacts of Ocean Acidification BIOACID Ocean Acidification International Coordination Centre OA-ICC |
description |
Exposure to elevated seawater PCO2 limits the thermal tolerance of crustaceans but the underlying mechanisms have not been comprehensively explored. Larval stages of crustaceans are even more sensitive to environmental hypercapnia and possess narrower thermal windows than adults. In a mechanistic approach, we analysed the impact of high seawater CO2 on parameters at different levels of biological organization, from the molecular to the whole animal level. At the whole animal level we measured oxygen consumption, heart rate and activity during acute warming in zoea and megalopa larvae of the spider crab Hyas araneus exposed to different levels of seawater PCO2. Furthermore, the expression of genes responsible for acid-base regulation and mitochondrial energy metabolism, and cellular responses to thermal stress (e.g. the heat shock response) was analysed before and after larvae were heat shocked byrapidly raising the seawater temperature from 10°C rearing temperature to 20°C. Zoea larvae showed a high heat tolerance, which decreased at elevated seawater PCO2, while the already low heat tolerance of megalopa larvae was not limited further by hypercapnic exposure. There was a combined effect of elevated seawater CO2 and heat shock in zoea larvae causing elevated transcript levels of heat shock proteins. In all three larval stages, hypercapnic exposure elicited an up-regulation of genes involved in oxidative phosphorylation, which was, however, not accompanied by increased energetic demands. The combined effect of seawater CO2 and heat shock on the gene expression of heat shock proteins reflects the downward shift in thermal limits seen on the whole animal level and indicates an associated capacity to elicit passive thermal tolerance. The up-regulation of genes involved in oxidative phosphorylation might compensate for enzyme activities being lowered through bicarbonate inhibition and maintain larval standard metabolic rates at high seawater CO2 levels. The present study underlines the necessity to align transcriptomic data with physiological responses when addressing mechanisms affected by an interaction of elevated seawater PCO2 and temperature extremes. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-06-04. |
format |
Dataset |
author |
Schiffer, Melanie |
author_facet |
Schiffer, Melanie |
author_sort |
Schiffer, Melanie |
title |
Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2, supplement to: Schiffer, Melanie; Harms, Lars; Lucassen, Magnus; Mark, Felix Christopher; Pörtner, Hans-Otto; Storch, Daniela (2014): Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2. Frontiers in Zoology, 11(1) |
title_short |
Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2, supplement to: Schiffer, Melanie; Harms, Lars; Lucassen, Magnus; Mark, Felix Christopher; Pörtner, Hans-Otto; Storch, Daniela (2014): Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2. Frontiers in Zoology, 11(1) |
title_full |
Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2, supplement to: Schiffer, Melanie; Harms, Lars; Lucassen, Magnus; Mark, Felix Christopher; Pörtner, Hans-Otto; Storch, Daniela (2014): Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2. Frontiers in Zoology, 11(1) |
title_fullStr |
Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2, supplement to: Schiffer, Melanie; Harms, Lars; Lucassen, Magnus; Mark, Felix Christopher; Pörtner, Hans-Otto; Storch, Daniela (2014): Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2. Frontiers in Zoology, 11(1) |
title_full_unstemmed |
Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2, supplement to: Schiffer, Melanie; Harms, Lars; Lucassen, Magnus; Mark, Felix Christopher; Pörtner, Hans-Otto; Storch, Daniela (2014): Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2. Frontiers in Zoology, 11(1) |
title_sort |
temperature tolerance of different larval stages of the spider crab hyas araneus exposed to elevated seawater pco2, supplement to: schiffer, melanie; harms, lars; lucassen, magnus; mark, felix christopher; pörtner, hans-otto; storch, daniela (2014): temperature tolerance of different larval stages of the spider crab hyas araneus exposed to elevated seawater pco2. frontiers in zoology, 11(1) |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2014 |
url |
https://dx.doi.org/10.1594/pangaea.846762 https://doi.pangaea.de/10.1594/PANGAEA.846762 |
genre |
North Atlantic Ocean acidification |
genre_facet |
North Atlantic Ocean acidification |
op_relation |
https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1186/s12983-014-0087-4 https://cran.r-project.org/package=seacarb |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.846762 https://doi.org/10.1186/s12983-014-0087-4 |
_version_ |
1766137383088029696 |
spelling |
ftdatacite:10.1594/pangaea.846762 2023-05-15T17:37:27+02:00 Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2, supplement to: Schiffer, Melanie; Harms, Lars; Lucassen, Magnus; Mark, Felix Christopher; Pörtner, Hans-Otto; Storch, Daniela (2014): Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2. Frontiers in Zoology, 11(1) Schiffer, Melanie 2014 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.846762 https://doi.pangaea.de/10.1594/PANGAEA.846762 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1186/s12983-014-0087-4 https://cran.r-project.org/package=seacarb Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Animalia Arthropoda Behaviour Bottles or small containers/Aquaria <20 L Coast and continental shelf Gene expression incl. proteomics Hyas araneus Laboratory experiment Mortality/Survival North Atlantic Pelagos Respiration Single species Temperate Temperature Zooplankton Species Table Figure Day of experiment Temperature, water Treatment Stage Oxygen consumption Heart beat rate Heart rate Mortality Gene name Gene expression Standard deviation Temperature, water, standard deviation pH pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Partial pressure of carbon dioxide water at sea surface temperature wet air Partial pressure of carbon dioxide, standard deviation Salinity Salinity, standard deviation Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Calculated using seacarb after Nisumaa et al. 2010 Biological Impacts of Ocean Acidification BIOACID Ocean Acidification International Coordination Centre OA-ICC Dataset dataset Supplementary Dataset 2014 ftdatacite https://doi.org/10.1594/pangaea.846762 https://doi.org/10.1186/s12983-014-0087-4 2022-02-09T13:12:42Z Exposure to elevated seawater PCO2 limits the thermal tolerance of crustaceans but the underlying mechanisms have not been comprehensively explored. Larval stages of crustaceans are even more sensitive to environmental hypercapnia and possess narrower thermal windows than adults. In a mechanistic approach, we analysed the impact of high seawater CO2 on parameters at different levels of biological organization, from the molecular to the whole animal level. At the whole animal level we measured oxygen consumption, heart rate and activity during acute warming in zoea and megalopa larvae of the spider crab Hyas araneus exposed to different levels of seawater PCO2. Furthermore, the expression of genes responsible for acid-base regulation and mitochondrial energy metabolism, and cellular responses to thermal stress (e.g. the heat shock response) was analysed before and after larvae were heat shocked byrapidly raising the seawater temperature from 10°C rearing temperature to 20°C. Zoea larvae showed a high heat tolerance, which decreased at elevated seawater PCO2, while the already low heat tolerance of megalopa larvae was not limited further by hypercapnic exposure. There was a combined effect of elevated seawater CO2 and heat shock in zoea larvae causing elevated transcript levels of heat shock proteins. In all three larval stages, hypercapnic exposure elicited an up-regulation of genes involved in oxidative phosphorylation, which was, however, not accompanied by increased energetic demands. The combined effect of seawater CO2 and heat shock on the gene expression of heat shock proteins reflects the downward shift in thermal limits seen on the whole animal level and indicates an associated capacity to elicit passive thermal tolerance. The up-regulation of genes involved in oxidative phosphorylation might compensate for enzyme activities being lowered through bicarbonate inhibition and maintain larval standard metabolic rates at high seawater CO2 levels. The present study underlines the necessity to align transcriptomic data with physiological responses when addressing mechanisms affected by an interaction of elevated seawater PCO2 and temperature extremes. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-06-04. Dataset North Atlantic Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |