No detectable effect of CO2 on elemental stoichiometry of Emiliania huxleyi in nutrient-limited, acclimated continuous cultures ...

• Main Authors: Engel, Anja, Cisternas Novoa, Carolina, Wurst, Mascha, Endres, Sonja, Tang, Tiantian, Schartau, Markus, Lee, Cindy
• Format: Dataset
• Language: English
• Published: PANGAEA 2014
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Emiliania huxleyi; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species; Species; Figure; Day of experiment; Carbon dioxide, partial pressure; Abundance per volume; Emiliania huxleyi, standard deviation; Chlorophyll a per cell; Chlorophyll a, standard deviation; Carbon, organic, particulate, per cell; Carbon, organic, particulate, standard deviation; Carbon, inorganic, particulate, per cell; Standard deviation; Nitrogen, particulate, per cell; Phosphorus, organic, particulate, per cell; Carbon, organic, dissolved, per cell; Nitrogen, organic, dissolved, per cell; Particulate inorganic carbon/particulate organic carbon ratio; Particulate inorganic carbon/particulate organic carbon ratio, standard deviation; Carbon, organic, particulate/Nitrogen, particulate ratio; Carbon, organic, particulate/Nitrogen, particulate ratio, standard deviation; Nitrogen, total, particulate/Phosphorus, organic, particulate, ratio; Nitrogen, total, particulate/Phosphorus, organic, particulate, ratio, standard deviation; Carbon, organic, dissolved/Nitrogen, organic, dissolved ratio; Salinity; Temperature, water; pH; pH, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.845818; https://doi.pangaea.de/10.1594/PANGAEA.845818

Effects of CO2 concentration on elemental composition of the coccolithophore Emiliania huxleyi were studied in phosphorus-limited, continuous cultures that were acclimated to experimental conditions for 30 d prior to the first sampling. We determined phytoplankton and bacterial cell numbers, nutrients, particulate components like organic carbon (POC), inorganic carbon (PIC), nitrogen (PN), organic phosphorus (POP), transparent exopolymer particles (TEP), as well as dissolved organic carbon (DOC) and nitrogen (DON), in addition to carbonate system parameters at CO2 levels of 180, 380 and 750 µatm. No significant difference between treatments was observed for any of the measured variables during repeated sampling over a 14 d period. We considered several factors that might lead to these results, i.e. light, nutrients, carbon overconsumption and transient versus steady-state growth. We suggest that the absence of a clear CO2 effect during this study does not necessarily imply the absence of an effect in nature. ... : This work was partly financed by ADAGIO http://www.somassbu.org/research/adagio/In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-04-20. ...