Shell condition and survival of Puget Sound pteropods are impaired by ocean acidification conditions, supplement to: Busch, Shallin D; Maher, Michael; Thibodeau, Patricia; McElhany, Paul; Hofmann, Gretchen E (2014): Shell Condition and Survival of Puget Sound Pteropods Are Impaired by Ocean Acidification Conditions. PLoS ONE, 9(8), e105884

• Main Authors: Busch, Shallin D, Maher, Michael, Thibodeau, Patricia, McElhany, Paul, Hofmann, Gretchen E
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Animalia; Bottles or small containers/Aquaria <20 L; Brackish waters; Calcification/Dissolution; Laboratory experiment; Limacina helicina; Mollusca; Mortality/Survival; North Pacific; Pelagos; Single species; Temperate; Zooplankton; Species; Date; Experiment; Treatment; Identification; Number of individuals; Ratio; Sample ID; Score; Replicates; Shell; Salinity; Temperature, water; Temperature, water, standard deviation; pH; pH, standard deviation; Fugacity of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Alkalinity, total; Carbon, inorganic, dissolved; Carbonate system computation flag; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Calcite saturation state; Potentiometric; Spectrophotometric; Calculated using CO2SYS; Potentiometric titration; Coulometric titration; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Hofmann; Maher; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.844092; https://doi.pangaea.de/10.1594/PANGAEA.844092

We tested whether the thecosome pteropod Limacina helicina from Puget Sound, an urbanized estuary in the northwest continental US, experiences shell dissolution and altered mortality rates when exposed to the high CO2, low aragonite saturation state (Omega a) conditions that occur in Puget Sound and the northeast Pacific Ocean. Five, week-long experiments were conducted in which we incubated pteropods collected from Puget Sound in four carbon chemistry conditions: current summer surface (460-500 µatm CO2, Omega a=1.59), current deep water or surface conditions during upwelling (760 and 1600-1700 µatm CO2, Omega a=1.17 and 0.56), and future deep water or surface conditions during upwelling (2800-3400 µatm CO2, Omega a=0.28). We measured shell condition using a scoring regime of five shell characteristics that capture different aspects of shell dissolution. We characterized carbon chemistry conditions in statistical analyses with Omega a, and conducted analyses considering Omega a both as a continuous dataset and as discrete treatments. Shell dissolution increased linearly as aragonite saturation state decreased. Discrete treatment comparisons indicate that shell dissolution was greater in undersaturated treatments compared to oversaturated treatments. Survival increased linearly with aragonite saturation state, though discrete treatment comparisons indicated that survival was similar in all but the lowest saturation state treatment. These results indicate that, under starvation conditions, pteropod survival may not be greatly affected by current and expected near-future aragonite saturation state in the NE Pacific, but shell dissolution may. Given that subsurface waters in Puget Sound's main basin are undersaturated with respect to aragonite in the winter and can be undersaturated in the summer, the condition and persistence of the species in this estuary warrants further study. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2015) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-03-12.