Chemical characterization and quantification of the brown algal storage compound laminarin ...

• Main Authors: Graiff, Angelika, Ruth, Wolfgang, Kragl, Udo, Karsten, Ulf
• Format: Dataset
• Language: English
• Published: PANGAEA 2015
• Subjects: Species; Tissues; Replicate; Laminarin; Glucose; Mannitol; Carbon; Nitrogen, total; Carbon/Nitrogen ratio; Sampling by hand; Biological Impacts of Ocean Acidification BIOACID; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.843456; https://doi.pangaea.de/10.1594/PANGAEA.843456

In search of a meaningful stress indicator for Fucus vesiculosus we found that the often used quantitative determination procedures for the polysaccharide laminarin (beta-1,3-glucan) result in different kind of problems, uncertainties and limitations. This chemical long-term storage form of carbon enables perennial brown algae in seasonally fluctuating ecosystems to uncouple growth from photosynthesis. Because of this high ecological relevance a reliable and precise method for determination and quantification of laminarin is needed. Therefore, a simple, cold water extraction method coupled to a new quantitative liquid chromatography-mass spectrometrical method (LC-MS) was developed. Laminarin was determined in nine out of twelve brown algal species, and its expected typical molar mass distribution of 2000-7000 Da was confirmed. Furthermore, laminarin consisted of a complex mixture of different chemical forms, since fifteen chemical laminarin species with distinct molecular weights were measured in nine ... : Supplement to: Graiff, Angelika; Ruth, Wolfgang; Kragl, Udo; Karsten, Ulf (2015): Chemical characterization and quantification of the brown algal storage compound laminarin — A new methodological approach. Journal of Applied Phycology ...