Impacts of elevated CO2 on particulate and dissolved organic matter production: microcosm experiments using iron-deficient plankton communities in open subarctic waters, supplement to: Yoshimura, T; Suzuki, Koji; Kiyosawa, H; Ono, Tsuneo; Hattori, Hiroshi; Kuma, Kenshi; Nishioka, Jun (2013): Impacts of elevated CO2 on particulate and dissolved organic matter production: microcosm experiments using iron-deficient plankton communities in open subarctic waters. Journal of Oceanography, 69(5), 601-618

• Main Authors: Yoshimura, T, Suzuki, Koji, Kiyosawa, H, Ono, Tsuneo, Hattori, Hiroshi, Kuma, Kenshi, Nishioka, Jun
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2013
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Ceratium spp.; Chaetoceros spp.; Community composition and diversity; Coscinodiscus sp.; Dinobryon balticum; Entire community; Gymnodinium spp.; Laboratory experiment; Mesodinium rubrum; Micro-nutrients; Neodenticula seminae; North Pacific; Open ocean; Parafavella spp.; Pelagos; Prorocentrum spp.; Pseudo-nitzschia spp.; Rhizosolenia spp.; Temperate; Thalassiothrix sp.; Tropidoneis spp.; Event label; Site; Time in days; Partial pressure of carbon dioxide water at sea surface temperature wet air; Replicate; Chlorophyll a; Ammonium; Nitrate and Nitrite; Nitrite; Phosphate; Silicate; Carbon, organic, particulate; Nitrogen, total, particulate; Carbon, organic, dissolved; Phosphorus, organic, dissolved; Fucoxanthin; 19-Hexanoyloxyfucoxanthin; Peridinin; Bacteria; Synechococcus; Eukaryotes; Diatoms; Haptophytes; Species; Cell density; Temperature, water; Salinity; Alkalinity, total; Carbon, inorganic, dissolved; Carbonate system computation flag; pH; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Experiment; Fluorescence determination; Spectrophotometric; High Performance Liquid Chromatography HPLC; Flow cytometry; Potentiometric titration; Coulometric titration; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Bering Sea; Pacific; Ocean acidification; Subarctic
• Online Access: https://dx.doi.org/10.1594/pangaea.842185; https://doi.pangaea.de/10.1594/PANGAEA.842185

Response of phytoplankton to increasing CO2 in seawater in terms of physiology and ecology is key to predicting changes in marine ecosystems. However, responses of natural plankton communities especially in the open ocean to higher CO2 levels have not been fully examined. We conducted CO2 manipulation experiments in the Bering Sea and the central subarctic Pacific, known as high nutrient and low chlorophyll regions, in summer 2007 to investigate the response of organic matter production in iron-deficient plankton communities to CO2 increases. During the 14-day incubations of surface waters with natural plankton assemblages in microcosms under multiple pCO2 levels, the dynamics of particulate organic carbon (POC) and nitrogen (PN), and dissolved organic carbon (DOC) and phosphorus (DOP) were examined with the plankton community compositions. In the Bering site, net production of POC, PN, and DOP relative to net chlorophyll-a production decreased with increasing pCO2. While net produced POC:PN did not show any CO2-related variations, net produced DOC:DOP increased with increasing pCO2. On the other hand, no apparent trends for these parameters were observed in the Pacific site. The contrasting results observed were probably due to the different plankton community compositions between the two sites, with plankton biomass dominated by large-sized diatoms in the Bering Sea versus ultra-eukaryotes in the Pacific Ocean. We conclude that the quantity and quality of the production of particulate and dissolved organic matter may be altered under future elevated CO2 environments in some iron-deficient ecosystems, while the impacts may be negligible in some systems. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2015-01-29.