Relative sensitivity of soft-bottom intertidal macrofauna to increased CO2 and experimental stress, supplement to: Range, P; Martins, M; Cabral, S; Pilò, D; Ben-Hamadou, R; Teodósio, M A; Leitão, F; Drago, Teresa; Oliveira, A P; Matias, D; ChÌcharo, L (2014): Relative sensitivity of soft-bottom intertidal macrofauna to increased CO2 and experimental stress. Marine Ecology Progress Series, 509, 153-170

• Main Authors: Range, P, Martins, M, Cabral, S, Pilò, D, Ben-Hamadou, R, Teodósio, M A, Leitão, F, Drago, Teresa, Oliveira, A P, Matias, D, ChÌcharo, L
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Abra nitida; Abra prismatica; Abra tenuis; Acteon tornatilis; Apohyale prevostii; Atylus swammerdami; Benthos; Biomass/Abundance/Elemental composition; Bittium spp.; Bulla striata; Calyptraea chinensis; Cerastoderma edule; Cerastoderma glaucum; Cerithium vulgatum; Coast and continental shelf; Community composition and diversity; Containers and aquaria 20-1000 L or < 1 m**2; Crangon crangon; Cyclope pellucida; Diogenes pugilator; Ensis siliqua; Entire community; Euphausia krohni; Haminoea hydatis; Laboratory experiment; Lekanesphaera monodi; Liocarcinus arcuatus; Loripes lacteus; Lutraria lutraria; Melita palmata; Mesalia mesal; Metapenaeus monoceros; Nassarius cuvieri; Nassarius incrassatus; Nassarius nitidus; Nassarius pfeifferi; Nassarius pygmaeus; Nassarius reticulatus; North Atlantic; Nucula nucleus; Nucula sulcata; Pachygrapsus marmoratus; Pagurus sp.; Paphia aurea; Paphia rhomboides; Parvicardium exiguum; Peringia ulvae; Pholas dactylus; Rissoa monodonta; Ruditapes decussatus; Scrobicularia plana; Soft-bottom community; Solen marginatus; Spisula subtruncata; Temperate; Upogebia pusilla; Date; Habitat; Replicate; Treatment; Taxon/taxa; Family; Genus; Species; Individuals; Abundance per area; Temperature, water; Temperature, water, standard error; Salinity; Salinity, standard error; pH; pH, standard error; Alkalinity, total; Alkalinity, total, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Calcite saturation state; Calcite saturation state, standard error; Aragonite saturation state; Aragonite saturation state, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Experiment; Potentiometric; Potentiometric titration; Calculated; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Drago; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.841042; https://doi.pangaea.de/10.1594/PANGAEA.841042

We used a controlled CO2 perturbation experiment to test hypotheses about changes in diversity, composition and structure of soft-bottom intertidal macrobenthic assemblages, under realistic and locally relevant scenarios of seawater acidification. Patches of undisturbed sediment were collected from 2 types of intertidal sedimentary habitat in the Ria Formosa coastal lagoon (South Portugal) and exposed to 2 levels of seawater acidification (pH reduced by 0.3 and 0.6 units) and 1 unmanipulated (control) level. After 75 d the assemblages differed significantly between the 2 types of sediment and between field controls and the ex situ treatments, but not among the 3 pH levels tested. The naturally high values of total alkalinity buffered seawater from the changes imposed on carbonate chemistry and may have contributed to offsetting acidification at the local scale. Observed differences on biota were strongly related to the organic matter content and grain-size of the sediments, particularly to the fractions of medium and coarse sand. Soft-bottom intertidal macrofauna was significantly affected by the stress of being held in an artificial environment, but not by CO2-induced seawater acidification. Given the previously observed variations in the sensitivities of marine organisms to seawater acidification, direct extrapolations of the present findings to different regions or other types of assemblages do not seem advisable. However, the contribution of ex situ studies to the assessment of ecosystem-level responses to environmental disturbances could generally be improved by incorporating adequate field controls in the experimental design. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-12-25.