1H NMR Metabolomics Reveals Contrasting Response by Male and Female Mussels Exposed to Reduced Seawater pH, Increased Temperature, and a Pathogen, supplement to: Ellis, Robert P; Spicer, John I; Byrne, Jonathan J; Sommer, Ulf; Viant, Mark R; White, Daniel; Widdicombe, Steve (2014): 1H NMR Metabolomics Reveals Contrasting Response by Male and Female Mussels Exposed to Reduced Seawater pH, Increased Temperature, and a Pathogen. Environmental Science & Technology, 48(12), 7044-7052

• Main Authors: Ellis, Robert P, Spicer, John I, Byrne, Jonathan J, Sommer, Ulf, Viant, Mark R, White, Daniel, Widdicombe, Steve
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Laboratory experiment; Mollusca; Mortality/Survival; Mytilus edulis; North Atlantic; Single species; Temperate; Temperature; Species; pH; Temperature, water; Individuals; Mortality; pH, standard error; Temperature, water, standard error; Salinity; Salinity, standard error; Alkalinity, total; Alkalinity, total, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Calcite saturation state; Calcite saturation state, standard error; Aragonite saturation state; Aragonite saturation state, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.838938; https://doi.pangaea.de/10.1594/PANGAEA.838938

Human activities are fundamentally altering the chemistry of the world's oceans. Ocean acidification (OA) is occurring against a background of warming and an increasing occurrence of disease outbreaks, posing a significant threat to marine organisms, communities, and ecosystems. In the current study, 1H NMR spectroscopy was used to investigate the response of the blue mussel, Mytilus edulis, to a 90-day exposure to reduced seawater pH and increased temperature, followed by a subsequent pathogenic challenge. Analysis of the metabolome revealed significant differences between male and female organisms. Furthermore, males and females are shown to respond differently to environmental stress. While males were significantly affected by reduced seawater pH, increased temperature, and a bacterial challenge, it was only a reduction in seawater pH that impacted females. Despite impacting males and females differently, stressors seem to act via a generalized stress response impacting both energy metabolism and osmotic balance in both sexes. This study therefore has important implications for the interpretation of metabolomic data in mussels, as well as the impact of environmental stress in marine invertebrates in general. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-11-17.