Adaptation of a globally important coccolithophore to ocean warming and acidification, supplement to: Gibbin, Emma M; Putnam, H M; Davy, Simon K; Gates, Ruth D (2014): Intracellular pH and its response to CO2-driven seawater acidification in symbiotic versus non-symbiotic coral cells. Journal of Experimental Biology, 217(11), 1963-1969
Regulating intracellular pH (pHi) is critical for optimising the metabolic activity of corals, yet mechanisms involved in pH regulation and the buffering capacity within coral cells are not well understood. Our study investigated how the presence of symbiotic dinoflagellates affects the response of...
Main Authors: | , , , |
---|---|
Format: | Dataset |
Language: | English |
Published: |
PANGAEA - Data Publisher for Earth & Environmental Science
2014
|
Subjects: | |
Online Access: | https://dx.doi.org/10.1594/pangaea.837880 https://doi.pangaea.de/10.1594/PANGAEA.837880 |
id |
ftdatacite:10.1594/pangaea.837880 |
---|---|
record_format |
openpolar |
spelling |
ftdatacite:10.1594/pangaea.837880 2023-05-15T17:51:27+02:00 Adaptation of a globally important coccolithophore to ocean warming and acidification, supplement to: Gibbin, Emma M; Putnam, H M; Davy, Simon K; Gates, Ruth D (2014): Intracellular pH and its response to CO2-driven seawater acidification in symbiotic versus non-symbiotic coral cells. Journal of Experimental Biology, 217(11), 1963-1969 Gibbin, Emma M Putnam, H M Davy, Simon K Gates, Ruth D 2014 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.837880 https://doi.pangaea.de/10.1594/PANGAEA.837880 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1242/jeb.099549 https://cran.r-project.org/package=seacarb Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Acid-base regulation Animalia Benthic animals Benthos Cnidaria Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Laboratory experiment North Pacific Pocillopora damicornis Single species Tropical Species Figure Treatment Time in minutes Replicate pH, extracellular pH change pH, intracellular Salinity Salinity, standard error pH pH, standard error Alkalinity, total Alkalinity, total, standard error Temperature, water Temperature, water, standard error Carbonate system computation flag Carbon dioxide Partial pressure of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Aragonite saturation state Calcite saturation state Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Supplementary Dataset dataset Dataset 2014 ftdatacite https://doi.org/10.1594/pangaea.837880 https://doi.org/10.1242/jeb.099549 2021-11-05T12:55:41Z Regulating intracellular pH (pHi) is critical for optimising the metabolic activity of corals, yet mechanisms involved in pH regulation and the buffering capacity within coral cells are not well understood. Our study investigated how the presence of symbiotic dinoflagellates affects the response of pHi to pCO2-driven seawater acidification in cells isolated from Pocillopora damicornis. Using the fluorescent dye BCECF-AM, in conjunction with confocal microscopy, we simultaneously characterised the response of pHi in host coral cells and their dinoflagellate symbionts, in symbiotic and non-symbiotic states under saturating light, with and without the photosynthetic inhibitor DCMU. Each treatment was run under control (pH 7.8) and CO2 acidified seawater conditions (decreasing pH from 7.8 - 6.8). After two hours of CO2 addition, by which time the external pH (pHe) had declined to 6.8, the dinoflagellate symbionts had increased their pHi by 0.5 pH units above control levels. In contrast, in both symbiotic and non-symbiotic host coral cells, 15 min of CO2 addition (0.2 pH unit drop in pHe) led to cytoplasmic acidosis equivalent to 0.4 pH units. Despite further seawater acidification over the duration of the experiment, the pHi of non-symbiotic coral cells did not change, though in host cells containing a symbiont cell the pHi recovered to control levels. This recovery was negated when cells were incubated with DCMU. Our results reveal that photosynthetic activity of the endosymbiont is tightly coupled with the ability of the host cell to recover from cellular acidosis after exposure to high CO2 / low pH. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-11-04. Dataset Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) Pacific |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Acid-base regulation Animalia Benthic animals Benthos Cnidaria Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Laboratory experiment North Pacific Pocillopora damicornis Single species Tropical Species Figure Treatment Time in minutes Replicate pH, extracellular pH change pH, intracellular Salinity Salinity, standard error pH pH, standard error Alkalinity, total Alkalinity, total, standard error Temperature, water Temperature, water, standard error Carbonate system computation flag Carbon dioxide Partial pressure of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Aragonite saturation state Calcite saturation state Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Acid-base regulation Animalia Benthic animals Benthos Cnidaria Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Laboratory experiment North Pacific Pocillopora damicornis Single species Tropical Species Figure Treatment Time in minutes Replicate pH, extracellular pH change pH, intracellular Salinity Salinity, standard error pH pH, standard error Alkalinity, total Alkalinity, total, standard error Temperature, water Temperature, water, standard error Carbonate system computation flag Carbon dioxide Partial pressure of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Aragonite saturation state Calcite saturation state Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Gibbin, Emma M Putnam, H M Davy, Simon K Gates, Ruth D Adaptation of a globally important coccolithophore to ocean warming and acidification, supplement to: Gibbin, Emma M; Putnam, H M; Davy, Simon K; Gates, Ruth D (2014): Intracellular pH and its response to CO2-driven seawater acidification in symbiotic versus non-symbiotic coral cells. Journal of Experimental Biology, 217(11), 1963-1969 |
topic_facet |
Acid-base regulation Animalia Benthic animals Benthos Cnidaria Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Laboratory experiment North Pacific Pocillopora damicornis Single species Tropical Species Figure Treatment Time in minutes Replicate pH, extracellular pH change pH, intracellular Salinity Salinity, standard error pH pH, standard error Alkalinity, total Alkalinity, total, standard error Temperature, water Temperature, water, standard error Carbonate system computation flag Carbon dioxide Partial pressure of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Carbon, inorganic, dissolved Aragonite saturation state Calcite saturation state Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
description |
Regulating intracellular pH (pHi) is critical for optimising the metabolic activity of corals, yet mechanisms involved in pH regulation and the buffering capacity within coral cells are not well understood. Our study investigated how the presence of symbiotic dinoflagellates affects the response of pHi to pCO2-driven seawater acidification in cells isolated from Pocillopora damicornis. Using the fluorescent dye BCECF-AM, in conjunction with confocal microscopy, we simultaneously characterised the response of pHi in host coral cells and their dinoflagellate symbionts, in symbiotic and non-symbiotic states under saturating light, with and without the photosynthetic inhibitor DCMU. Each treatment was run under control (pH 7.8) and CO2 acidified seawater conditions (decreasing pH from 7.8 - 6.8). After two hours of CO2 addition, by which time the external pH (pHe) had declined to 6.8, the dinoflagellate symbionts had increased their pHi by 0.5 pH units above control levels. In contrast, in both symbiotic and non-symbiotic host coral cells, 15 min of CO2 addition (0.2 pH unit drop in pHe) led to cytoplasmic acidosis equivalent to 0.4 pH units. Despite further seawater acidification over the duration of the experiment, the pHi of non-symbiotic coral cells did not change, though in host cells containing a symbiont cell the pHi recovered to control levels. This recovery was negated when cells were incubated with DCMU. Our results reveal that photosynthetic activity of the endosymbiont is tightly coupled with the ability of the host cell to recover from cellular acidosis after exposure to high CO2 / low pH. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-11-04. |
format |
Dataset |
author |
Gibbin, Emma M Putnam, H M Davy, Simon K Gates, Ruth D |
author_facet |
Gibbin, Emma M Putnam, H M Davy, Simon K Gates, Ruth D |
author_sort |
Gibbin, Emma M |
title |
Adaptation of a globally important coccolithophore to ocean warming and acidification, supplement to: Gibbin, Emma M; Putnam, H M; Davy, Simon K; Gates, Ruth D (2014): Intracellular pH and its response to CO2-driven seawater acidification in symbiotic versus non-symbiotic coral cells. Journal of Experimental Biology, 217(11), 1963-1969 |
title_short |
Adaptation of a globally important coccolithophore to ocean warming and acidification, supplement to: Gibbin, Emma M; Putnam, H M; Davy, Simon K; Gates, Ruth D (2014): Intracellular pH and its response to CO2-driven seawater acidification in symbiotic versus non-symbiotic coral cells. Journal of Experimental Biology, 217(11), 1963-1969 |
title_full |
Adaptation of a globally important coccolithophore to ocean warming and acidification, supplement to: Gibbin, Emma M; Putnam, H M; Davy, Simon K; Gates, Ruth D (2014): Intracellular pH and its response to CO2-driven seawater acidification in symbiotic versus non-symbiotic coral cells. Journal of Experimental Biology, 217(11), 1963-1969 |
title_fullStr |
Adaptation of a globally important coccolithophore to ocean warming and acidification, supplement to: Gibbin, Emma M; Putnam, H M; Davy, Simon K; Gates, Ruth D (2014): Intracellular pH and its response to CO2-driven seawater acidification in symbiotic versus non-symbiotic coral cells. Journal of Experimental Biology, 217(11), 1963-1969 |
title_full_unstemmed |
Adaptation of a globally important coccolithophore to ocean warming and acidification, supplement to: Gibbin, Emma M; Putnam, H M; Davy, Simon K; Gates, Ruth D (2014): Intracellular pH and its response to CO2-driven seawater acidification in symbiotic versus non-symbiotic coral cells. Journal of Experimental Biology, 217(11), 1963-1969 |
title_sort |
adaptation of a globally important coccolithophore to ocean warming and acidification, supplement to: gibbin, emma m; putnam, h m; davy, simon k; gates, ruth d (2014): intracellular ph and its response to co2-driven seawater acidification in symbiotic versus non-symbiotic coral cells. journal of experimental biology, 217(11), 1963-1969 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2014 |
url |
https://dx.doi.org/10.1594/pangaea.837880 https://doi.pangaea.de/10.1594/PANGAEA.837880 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1242/jeb.099549 https://cran.r-project.org/package=seacarb |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.837880 https://doi.org/10.1242/jeb.099549 |
_version_ |
1766158600922726400 |