Regulation of intracellular pH in cnidarians: response to acidosis in Anemonia viridis ...

• Main Authors: Laurent, Julien, Venn, Alexander A, Tambutté, Eric, Ganot, Philippe, Allemand, Denis, Tambutté, Sylvie
• Format: Dataset
• Language: English
• Published: PANGAEA 2013
• Subjects: Acid-base regulation; Anemonia viridis; Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Cnidaria; Coast and continental shelf; Laboratory experiment; Mediterranean Sea; Respiration; Single species; Temperate; Species; Figure; Treatment; Time in minutes; pH, intracellular; pH, intracellular, standard error; Respiration rate, oxygen, per protein; Respiration rate, oxygen, standard error; Temperature, water; Salinity; pH; pH, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Potentiometric; Spectrophotometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.837087; https://doi.pangaea.de/10.1594/PANGAEA.837087

The regulation of intracellular pH (pHi) is a fundamental aspect of cell physiology that has received little attention in studies of the phylum Cnidaria, which includes ecologically important sea anemones and reef-building corals. Like all organisms, cnidarians must maintain pH homeostasis to counterbalance reductions in pHi, which can arise because of changes in either intrinsic or extrinsic parameters. Corals and sea anemones face natural daily changes in internal fluids, where the extracellular pH can range from 8.9 during the day to 7.4 at night. Furthermore, cnidarians are likely to experience future CO2-driven declines in seawater pH, a process known as ocean acidification. Here, we carried out the first mechanistic investigation to determine how cnidarian pHi regulation responds to decreases in extracellular and intracellular pH. Using the anemone Anemonia viridis, we employed confocal live cell imaging and a pH-sensitive dye to track the dynamics of pHi after intracellular acidosis induced by acute ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-10-23. ...