Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects, supplement to: Kottmeier, Dorothee; Rokitta, Sebastian D; Tortell, Philippe Daniel; Rost, Bjoern (2014): Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects. Photosynthesis Research, 121(2-3), 265-275
Effects of ocean acidification on Emiliania huxleyi strain RCC 1216 (calcifying, diploid life-cycle stage) and RCC 1217 (non-calcifying, haploid life-cycle stage) were investigated by measuring growth, elemental composition, and production rates under different pCO2 levels (380 and 950 µatm). In the...
Main Authors: | , , , |
---|---|
Format: | Dataset |
Language: | English |
Published: |
PANGAEA - Data Publisher for Earth & Environmental Science
2014
|
Subjects: | |
Online Access: | https://dx.doi.org/10.1594/pangaea.836912 https://doi.pangaea.de/10.1594/PANGAEA.836912 |
id |
ftdatacite:10.1594/pangaea.836912 |
---|---|
record_format |
openpolar |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Chromista Emiliania huxleyi Growth/Morphology Haptophyta Laboratory experiment Laboratory strains North Atlantic Other metabolic rates Pelagos Phytoplankton Single species Species Strain Treatment Salinity Temperature, water Phosphate Silicate Pressure, water Irradiance pH Alkalinity, total Carbon, inorganic, dissolved Growth rate Carbon, organic, particulate, per cell Total particulate carbon per cell Nitrogen, organic, particulate, per cell Chlorophyll a per cell LightDark cycle Carbon dioxide usage fraction Carbonate system computation flag Carbon dioxide Partial pressure of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Aragonite saturation state Calcite saturation state Potentiometric Potentiometric titration Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Chromista Emiliania huxleyi Growth/Morphology Haptophyta Laboratory experiment Laboratory strains North Atlantic Other metabolic rates Pelagos Phytoplankton Single species Species Strain Treatment Salinity Temperature, water Phosphate Silicate Pressure, water Irradiance pH Alkalinity, total Carbon, inorganic, dissolved Growth rate Carbon, organic, particulate, per cell Total particulate carbon per cell Nitrogen, organic, particulate, per cell Chlorophyll a per cell LightDark cycle Carbon dioxide usage fraction Carbonate system computation flag Carbon dioxide Partial pressure of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Aragonite saturation state Calcite saturation state Potentiometric Potentiometric titration Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Kottmeier, Dorothee Rokitta, Sebastian D Tortell, Philippe Daniel Rost, Bjoern Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects, supplement to: Kottmeier, Dorothee; Rokitta, Sebastian D; Tortell, Philippe Daniel; Rost, Bjoern (2014): Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects. Photosynthesis Research, 121(2-3), 265-275 |
topic_facet |
Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Chromista Emiliania huxleyi Growth/Morphology Haptophyta Laboratory experiment Laboratory strains North Atlantic Other metabolic rates Pelagos Phytoplankton Single species Species Strain Treatment Salinity Temperature, water Phosphate Silicate Pressure, water Irradiance pH Alkalinity, total Carbon, inorganic, dissolved Growth rate Carbon, organic, particulate, per cell Total particulate carbon per cell Nitrogen, organic, particulate, per cell Chlorophyll a per cell LightDark cycle Carbon dioxide usage fraction Carbonate system computation flag Carbon dioxide Partial pressure of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Aragonite saturation state Calcite saturation state Potentiometric Potentiometric titration Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
description |
Effects of ocean acidification on Emiliania huxleyi strain RCC 1216 (calcifying, diploid life-cycle stage) and RCC 1217 (non-calcifying, haploid life-cycle stage) were investigated by measuring growth, elemental composition, and production rates under different pCO2 levels (380 and 950 µatm). In these differently acclimated cells, the photosynthetic carbon source was assessed by a (14)C disequilibrium assay, conducted over a range of ecologically relevant pH values (7.9-8.7). In agreement with previous studies, we observed decreased calcification and stimulated biomass production in diploid cells under high pCO2, but no CO2-dependent changes in biomass production for haploid cells. In both life-cycle stages, the relative contributions of CO2 and HCO3 (-) uptake depended strongly on the assay pH. At pH values =< 8.1, cells preferentially used CO2 (>= 90 % CO2), whereas at pH values >= 8.3, cells progressively increased the fraction of HCO3 (-) uptake (~45 % CO2 at pH 8.7 in diploid cells; ~55 % CO2 at pH 8.5 in haploid cells). In contrast to the short-term effect of the assay pH, the pCO2 acclimation history had no significant effect on the carbon uptake behavior. A numerical sensitivity study confirmed that the pH-modification in the (14)C disequilibrium method yields reliable results, provided that model parameters (e.g., pH, temperature) are kept within typical measurement uncertainties. Our results demonstrate a high plasticity of E. huxleyi to rapidly adjust carbon acquisition to the external carbon supply and/or pH, and provide an explanation for the paradoxical observation of high CO2 sensitivity despite the apparently high HCO3 (-) usage seen in previous studies. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-30. |
format |
Dataset |
author |
Kottmeier, Dorothee Rokitta, Sebastian D Tortell, Philippe Daniel Rost, Bjoern |
author_facet |
Kottmeier, Dorothee Rokitta, Sebastian D Tortell, Philippe Daniel Rost, Bjoern |
author_sort |
Kottmeier, Dorothee |
title |
Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects, supplement to: Kottmeier, Dorothee; Rokitta, Sebastian D; Tortell, Philippe Daniel; Rost, Bjoern (2014): Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects. Photosynthesis Research, 121(2-3), 265-275 |
title_short |
Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects, supplement to: Kottmeier, Dorothee; Rokitta, Sebastian D; Tortell, Philippe Daniel; Rost, Bjoern (2014): Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects. Photosynthesis Research, 121(2-3), 265-275 |
title_full |
Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects, supplement to: Kottmeier, Dorothee; Rokitta, Sebastian D; Tortell, Philippe Daniel; Rost, Bjoern (2014): Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects. Photosynthesis Research, 121(2-3), 265-275 |
title_fullStr |
Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects, supplement to: Kottmeier, Dorothee; Rokitta, Sebastian D; Tortell, Philippe Daniel; Rost, Bjoern (2014): Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects. Photosynthesis Research, 121(2-3), 265-275 |
title_full_unstemmed |
Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects, supplement to: Kottmeier, Dorothee; Rokitta, Sebastian D; Tortell, Philippe Daniel; Rost, Bjoern (2014): Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects. Photosynthesis Research, 121(2-3), 265-275 |
title_sort |
strong shift from hco3- to co2 uptake in emiliania huxleyi with acidification: new approach unravels acclimation versus short-term ph effects, supplement to: kottmeier, dorothee; rokitta, sebastian d; tortell, philippe daniel; rost, bjoern (2014): strong shift from hco3- to co2 uptake in emiliania huxleyi with acidification: new approach unravels acclimation versus short-term ph effects. photosynthesis research, 121(2-3), 265-275 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2014 |
url |
https://dx.doi.org/10.1594/pangaea.836912 https://doi.pangaea.de/10.1594/PANGAEA.836912 |
genre |
North Atlantic Ocean acidification |
genre_facet |
North Atlantic Ocean acidification |
op_relation |
https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1007/s11120-014-9984-9 https://cran.r-project.org/package=seacarb |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.836912 https://doi.org/10.1007/s11120-014-9984-9 |
_version_ |
1766137279622938624 |
spelling |
ftdatacite:10.1594/pangaea.836912 2023-05-15T17:37:22+02:00 Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects, supplement to: Kottmeier, Dorothee; Rokitta, Sebastian D; Tortell, Philippe Daniel; Rost, Bjoern (2014): Strong shift from HCO3- to CO2 uptake in Emiliania huxleyi with acidification: new approach unravels acclimation versus short-term pH effects. Photosynthesis Research, 121(2-3), 265-275 Kottmeier, Dorothee Rokitta, Sebastian D Tortell, Philippe Daniel Rost, Bjoern 2014 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.836912 https://doi.pangaea.de/10.1594/PANGAEA.836912 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1007/s11120-014-9984-9 https://cran.r-project.org/package=seacarb Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Chromista Emiliania huxleyi Growth/Morphology Haptophyta Laboratory experiment Laboratory strains North Atlantic Other metabolic rates Pelagos Phytoplankton Single species Species Strain Treatment Salinity Temperature, water Phosphate Silicate Pressure, water Irradiance pH Alkalinity, total Carbon, inorganic, dissolved Growth rate Carbon, organic, particulate, per cell Total particulate carbon per cell Nitrogen, organic, particulate, per cell Chlorophyll a per cell LightDark cycle Carbon dioxide usage fraction Carbonate system computation flag Carbon dioxide Partial pressure of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Aragonite saturation state Calcite saturation state Potentiometric Potentiometric titration Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Supplementary Dataset dataset Dataset 2014 ftdatacite https://doi.org/10.1594/pangaea.836912 https://doi.org/10.1007/s11120-014-9984-9 2022-02-08T16:27:35Z Effects of ocean acidification on Emiliania huxleyi strain RCC 1216 (calcifying, diploid life-cycle stage) and RCC 1217 (non-calcifying, haploid life-cycle stage) were investigated by measuring growth, elemental composition, and production rates under different pCO2 levels (380 and 950 µatm). In these differently acclimated cells, the photosynthetic carbon source was assessed by a (14)C disequilibrium assay, conducted over a range of ecologically relevant pH values (7.9-8.7). In agreement with previous studies, we observed decreased calcification and stimulated biomass production in diploid cells under high pCO2, but no CO2-dependent changes in biomass production for haploid cells. In both life-cycle stages, the relative contributions of CO2 and HCO3 (-) uptake depended strongly on the assay pH. At pH values =< 8.1, cells preferentially used CO2 (>= 90 % CO2), whereas at pH values >= 8.3, cells progressively increased the fraction of HCO3 (-) uptake (~45 % CO2 at pH 8.7 in diploid cells; ~55 % CO2 at pH 8.5 in haploid cells). In contrast to the short-term effect of the assay pH, the pCO2 acclimation history had no significant effect on the carbon uptake behavior. A numerical sensitivity study confirmed that the pH-modification in the (14)C disequilibrium method yields reliable results, provided that model parameters (e.g., pH, temperature) are kept within typical measurement uncertainties. Our results demonstrate a high plasticity of E. huxleyi to rapidly adjust carbon acquisition to the external carbon supply and/or pH, and provide an explanation for the paradoxical observation of high CO2 sensitivity despite the apparently high HCO3 (-) usage seen in previous studies. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-30. Dataset North Atlantic Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |