Uranium in larval shells as a barometer of molluscan ocean acidification exposure, supplement to: Frieder, Christina A; Gonzalez, Jennifer P; Levin, Lisa A (2014): Uranium in Larval Shells as a barometer of Molluscan Ocean Acidification Exposure. Environmental Science & Technology, 48(11), 6401-6408

• Main Authors: Frieder, Christina A, Gonzalez, Jennifer P, Levin, Lisa A
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Animalia; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Laboratory experiment; Mollusca; Mytilus californianus; Mytilus galloprovincialis; North Pacific; Pelagos; Single species; Temperate; Zooplankton; Species; Treatment; Sample ID; Replicate; Magnesium/Calcium ratio; Copper/Calcium ratio; Zinc/Calcium ratio; Strontium/Calcium ratio; Barium/Calcium ratio; Lead/Calcium ratio; Uranium/Calcium ratio; pH; Oxygen; Temperature, water; Salinity; Alkalinity, total; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Spectrophotometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Gonzalez; Levin; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.836887; https://doi.pangaea.de/10.1594/PANGAEA.836887

As the ocean undergoes acidification, marine organisms will become increasingly exposed to reduced pH, yet variability in many coastal settings complicates our ability to accurately estimate pH exposure for those organisms that are difficult to track. Here we present shell-based geochemical proxies that reflect pH exposure from laboratory and field settings in larvae of the mussels Mytilus californianus and M. galloprovincialis. Laboratory-based proxies were generated from shells precipitated at pH 7.51 to 8.04. U/Ca, Sr/Ca, and multielemental signatures represented as principal components varied with pH for both species. Of these, U/Ca was the best predictor of pH and did not vary with larval size, with semidiurnal pH fluctuations, or with oxygen concentration. Field applications of U/Ca were tested with mussel larvae reared in situ at both known and unknown pH conditions. Larval shells precipitated in a region of greater upwelling had higher U/Ca, and these U/Ca values corresponded well with the laboratory-derived U/Ca-pH proxy. Retention of the larval shell after settlement in molluscs allows use of this geochemical proxy to assess ocean acidification effects on marine populations. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-10-17.