Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii (Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system, supplement to: Isensee, Kirsten; Erez, Jonathan; Stoll, Heather M (2014): Detection of a variable intracellular acid-labile carbon pool in Thalassiosira weissflogii(Heterokontophyta) and Emiliania huxleyi (Haptophyta) in response to changes in the seawater carbon system. Physiologia Plantarum, 150(2), 321-338

• Main Authors: Isensee, Kirsten, Erez, Jonathan, Stoll, Heather M
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Chromista; Emiliania huxleyi; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Ochrophyta; Other metabolic rates; Pelagos; Phytoplankton; Single species; Thalassiosira weissflogii; Table; Figure; Species; Treatment; pH; Carbon incorporation rate per cell; Temperature, water; Salinity; Alkalinity, total; Carbon, inorganic, dissolved; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Cell density; Cell biovolume; Chlorophyll a per cell; Carbon, intracellular pool per cell; Carbon, intracellular pool per cell, standard deviation; Ratio; Time in minutes; Replicate; Carbon-14 incorporation per cell; Time in seconds; Carbon-14, organic; Carbon-14, organic, standard deviation; Carbon dioxide/Bicarbonate uptake ratio; Carbon dioxide/Bicarbonate uptake ratio, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Fryxell; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.836733; https://doi.pangaea.de/10.1594/PANGAEA.836733

Accumulation of an intracellular pool of carbon (C(i) pool) is one strategy by which marine algae overcome the low abundance of dissolved CO2 (CO2 (aq) ) in modern seawater. To identify the environmental conditions under which algae accumulate an acid-labile C(i) pool, we applied a (14) C pulse-chase method, used originally in dinoflagellates, to two new classes of algae, coccolithophorids and diatoms. This method measures the carbon accumulation inside the cells without altering the medium carbon chemistry or culture cell density. We found that the diatom Thalassiosira weissflogii [(Grunow) G. Fryxell & Hasle] and a calcifying strain of the coccolithophorid Emiliania huxleyi [(Lohmann) W. W. Hay & H. P. Mohler] develop significant acid-labile C(i) pools. C(i) pools are measureable in cells cultured in media with 2-30 µmol/l CO2 (aq), corresponding to a medium pH of 8.6-7.9. The absolute C(i) pool was greater for the larger celled diatoms. For both algal classes, the C(i) pool became a negligible contributor to photosynthesis once CO2 (aq) exceeded 30 µmol/l. Combining the (14) C pulse-chase method and (14) C disequilibrium method enabled us to assess whether E. huxleyi and T. weissflogii exhibited thresholds for foregoing accumulation of DIC or reduced the reliance on bicarbonate uptake with increasing CO2 (aq) . We showed that the C(i) pool decreases with higher CO2 :HCO3 (-) uptake rates. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-10-15.