Proteomic and metabolomic responses of Pacific oyster Crassostrea gigas to elevated pCO2 exposure ...

• Main Authors: Wei, Lei, Wang, Qing, Wu, Huifeng, Ji, Chenglong, Zhao, Jianmin
• Format: Dataset
• Language: English
• Published: PANGAEA 2015
• Subjects: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Crassostrea gigas; Gene expression incl. proteomics; Laboratory experiment; Mollusca; North Pacific; Single species; Temperate; Species; Treatment; Tissues; Protein name; mRNA gene expression, relative; mRNA gene expression, relative, standard deviation; Temperature, water; Salinity; pH, NBS scale; Carbon, inorganic, dissolved; Alkalinity, total; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbonate system computation flag; pH, total scale; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Potentiometric; Coulometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification; Pacific oyster
• Online Access: https://dx.doi.org/10.1594/pangaea.836666; https://doi.pangaea.de/10.1594/PANGAEA.836666

The gradually increased atmospheric CO2 partial pressure (pCO2) has thrown the carbonate chemistry off balance and resulted in decreased seawater pH in marine ecosystem, termed ocean acidification (OA). Anthropogenic OA is postulated to affect the physiology of many marine calcifying organisms. However, the susceptibility and metabolic pathways of change in most calcifying animals are still far from being well understood. In this work, the effects of exposure to elevated pCO2 were characterized in gills and hepatopancreas of Crassostrea gigas using integrated proteomic and metabolomic approaches. Metabolic responses indicated that high CO2 exposure mainly caused disturbances in energy metabolism and osmotic regulation marked by differentially altered ATP, glucose, glycogen, amino acids and organic osmolytes in oysters, and the depletions of ATP in gills and the accumulations of ATP, glucose and glycogen in hepatopancreas accounted for the difference in energy distribution between these two tissues. Proteomic ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-10-13. ...