Land based mesocosm experiment studying the simultaneous impact of warming and acidification on the planktonic food web of the Eastern Mediterranean ...

• Main Authors: Pitta, Paraskevi, Krasakopoulou, Evangelia, Frangoulis, Constantin, Ziveri, Patrizia
• Format: Dataset
• Language: English
• Published: PANGAEA 2014
• Subjects: DATE/TIME; Comment; DEPTH, water; Experimental treatment; Salinity; Temperature, water; pH; Carbon dioxide; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Alkalinity, total; Aragonite saturation state; Calcite saturation state; Nitrite; Nitrate; Ammonia; Phosphate; Silicate; Nitrogen, total, particulate; Carbon, organic, particulate; Bacterial production; Bacteria, heterotrophic; Synechococcus; Picoeukaryotes, autotrophic; Viral abundance; Primary production of carbon per hour; Primary production of carbon per hour, fractionated; Chlorophyll a; Chlorophyll a, fractionated; Phosphate turnover time; Phosphate uptake, fractionated; Production of copepoda; Nitrogen fixation rate; Mesocosm experiment; Conductivity sensor, Aanderaa, type 3919; Pendant Temperature/Light Data Logger 64K, HOBO; pH meter Metrohm electrodes; Calculated using seacarb after Nisumaa et al. 2010; Coulometric titration, Marianda, VINDTA 3C; Seawater analysis Strickland & Parsons, 1972; Seawater analysis Ivancic & Deggobis, 1984; Spectrophotometry, MAGIC25 procedure Rimmelin & Moutin, 2005; Element analyser, Thermo Scientific FLASH2000; see comment; Flow cytometry; Steemann-Nielsen 1952 use of 14C for measuring org. prod. J Conseil 18117; Fluorescence determination; Conseil; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.836005; https://doi.pangaea.de/10.1594/PANGAEA.836005

A land based mesocosm experiment focusing on the study of the simultaneous impact of warming and acidification on the planktonic food web of the Eastern Mediterranean took place in August-September 2013 at the mesocosm facilities of HCMR in Crete (CRETACOSMOS). Two different pCO2 (present day and predicted for year 2100) were applied in triplicate mesocosms of 3 m**3. This was tested in two different temperatures (ambient seawater T and ambient T plus 3°C). Twelve mesocosms in total were incubated in two large concrete tanks. Temperature was controlled by sophisticated, automated systems. A large variety of chemical, biological and biochemical variables were studied, including salinity, temperature, light and alkalinity measurements, inorganic and organic, particulate and dissolved, nutrient analyses, biological stock (Chla concentration, enumeration and community composition of microbial, phyto- and zooplankton organisms) and rate (primary, bacterial, viral production, copepod egg production, zooplankton ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). ...