Effects of hypercapnia on aspects of feeding, nutrition, and growth in the edible sea urchin Lytechinus variegatus held in culture, supplement to: Challener, Roberta C; Watts, Stephen A; McClintock, James B (2014): Effects of hypercapnia on aspects of feeding, nutrition, and growth in the edible sea urchin Lytechinus variegatus held in culture. Marine and Freshwater Behaviour and Physiology, 47(1), 41-62

• Main Authors: Challener, Roberta C, Watts, Stephen A, McClintock, James B
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Echinodermata; Growth/Morphology; Laboratory experiment; Lytechinus variegatus; North Atlantic; Single species; Temperate; Species; Figure; Table; Treatment; Identification; Incubation duration; Feed, dry, total; Feces, dry, total; Digestibility; Wet mass; Feeding rate; Fecal production rate; Energy; Absolute lipid content; Absolute protein content; Carbohydrates mass; Ash mass; Food mass; Energy, per food mass; Dry mass; Ash; Carbohydrates; Lipids; Protein; Organic matter; Diameter; Inorganic matter; pH; pH, standard error; Temperature, water; Temperature, water, standard error; Oxygen; Oxygen, standard error; Salinity; Salinity, standard error; Alkalinity, total; Alkalinity, total, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Calcite saturation state; Calcite saturation state, standard error; Aragonite saturation state; Aragonite saturation state, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Experiment; Potentiometric; Calculated; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; McClintock; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.835643; https://doi.pangaea.de/10.1594/PANGAEA.835643

Land-based aquaculture facilities experience occasional hypercapnic conditions due to the accumulation of the metabolic waste product carbon dioxide. Pre-gonadal Lytechinus variegatus (horizontal diameter=20 mm) were exposed to control (608 µatm pCO2, pH 8.1) or hypercapnic conditions (1738 µatm pCO2, pH 7.7) in synthetic seawater for 14 weeks. Sea urchins exposed to hypercapnic conditions exhibited significantly slower growth (reduced dry matter production), primarily due to reduced test production. Higher fecal production rates and lower ash absorption efficiency (%) in individuals exposed to hypercapnic conditions suggest the ability to process or retain dietary carbonates may have been affected. Significant increases in neutral lipid storage in the gut and increased soluble protein storage in the gonads of individuals exposed to hypercapnic conditions suggest alterations in nutrient metabolism and storage. Furthermore, organic production and energy allocation increased in the lantern of those individuals exposed to hypercapnic conditions. These results suggest chronic exposure to hypercapnic conditions alters nutrient allocation to organ systems and functions, leading to changes in somatic and reproductive production. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-10.