CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom
Phytoplankton growth can be limited by numerous inorganic nutrients and organic growth factors. Using the subarctic diatom Attheya sp. in culture studies, we examined how the availability of vitamin B(12) and carbon dioxide partial pressure (pCO(2)) influences growth rate, primary productivity, cell...
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Language: | English |
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PANGAEA - Data Publisher for Earth & Environmental Science
2011
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Online Access: | https://dx.doi.org/10.1594/pangaea.835475 https://doi.pangaea.de/10.1594/PANGAEA.835475 |
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ftdatacite:10.1594/pangaea.835475 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Attheya sp. Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Chromista Growth/Morphology Laboratory experiment Laboratory strains Micro-nutrients North Pacific Ochrophyta Pelagos Phytoplankton Primary production/Photosynthesis Single species Species Treatment Partial pressure of carbon dioxide water at sea surface temperature wet air Growth rate Growth rate, standard deviation Primary production Primary production of carbon, standard deviation Carbon/Phosphorus ratio Carbon/Phosphorus ratio, standard deviation Nitrogen/Phosphorus ratio Nitrogen/Phosphorus ratio, standard deviation Iron/Phosphorus ratio Iron/Phosphorus ratio, standard deviation Cobalt/Phosphorus ratio Cobalt/Phosphorus ratio, standard deviation Zinc/Phosphorus ratio Zinc/Phosphorus ratio, standard deviation Cadmium/Phosphorus ratio Cadmium/Phosphorus ratio, standard deviation Net use efficiency, Iron Net use efficiency, Iron, standard deviation Net use efficiency, Cobalt Net use efficiency, Cobalt, standard deviation Net use efficiency, Zinc Net use efficiency, Zinc, standard deviation Net use efficiency, Cadmium Net use efficiency, Cadmium, standard deviation Salinity Temperature, water pH pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Partial pressure of carbon dioxide, standard deviation Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Potentiometric Coulometric titration Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Attheya sp. Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Chromista Growth/Morphology Laboratory experiment Laboratory strains Micro-nutrients North Pacific Ochrophyta Pelagos Phytoplankton Primary production/Photosynthesis Single species Species Treatment Partial pressure of carbon dioxide water at sea surface temperature wet air Growth rate Growth rate, standard deviation Primary production Primary production of carbon, standard deviation Carbon/Phosphorus ratio Carbon/Phosphorus ratio, standard deviation Nitrogen/Phosphorus ratio Nitrogen/Phosphorus ratio, standard deviation Iron/Phosphorus ratio Iron/Phosphorus ratio, standard deviation Cobalt/Phosphorus ratio Cobalt/Phosphorus ratio, standard deviation Zinc/Phosphorus ratio Zinc/Phosphorus ratio, standard deviation Cadmium/Phosphorus ratio Cadmium/Phosphorus ratio, standard deviation Net use efficiency, Iron Net use efficiency, Iron, standard deviation Net use efficiency, Cobalt Net use efficiency, Cobalt, standard deviation Net use efficiency, Zinc Net use efficiency, Zinc, standard deviation Net use efficiency, Cadmium Net use efficiency, Cadmium, standard deviation Salinity Temperature, water pH pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Partial pressure of carbon dioxide, standard deviation Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Potentiometric Coulometric titration Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC King, Andrew L Sañudo-Wilhelmy, Sergio A Leblanc, Karine Hutchins, David A Fu, Feixue CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
topic_facet |
Attheya sp. Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Chromista Growth/Morphology Laboratory experiment Laboratory strains Micro-nutrients North Pacific Ochrophyta Pelagos Phytoplankton Primary production/Photosynthesis Single species Species Treatment Partial pressure of carbon dioxide water at sea surface temperature wet air Growth rate Growth rate, standard deviation Primary production Primary production of carbon, standard deviation Carbon/Phosphorus ratio Carbon/Phosphorus ratio, standard deviation Nitrogen/Phosphorus ratio Nitrogen/Phosphorus ratio, standard deviation Iron/Phosphorus ratio Iron/Phosphorus ratio, standard deviation Cobalt/Phosphorus ratio Cobalt/Phosphorus ratio, standard deviation Zinc/Phosphorus ratio Zinc/Phosphorus ratio, standard deviation Cadmium/Phosphorus ratio Cadmium/Phosphorus ratio, standard deviation Net use efficiency, Iron Net use efficiency, Iron, standard deviation Net use efficiency, Cobalt Net use efficiency, Cobalt, standard deviation Net use efficiency, Zinc Net use efficiency, Zinc, standard deviation Net use efficiency, Cadmium Net use efficiency, Cadmium, standard deviation Salinity Temperature, water pH pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Partial pressure of carbon dioxide, standard deviation Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Potentiometric Coulometric titration Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
description |
Phytoplankton growth can be limited by numerous inorganic nutrients and organic growth factors. Using the subarctic diatom Attheya sp. in culture studies, we examined how the availability of vitamin B(12) and carbon dioxide partial pressure (pCO(2)) influences growth rate, primary productivity, cellular iron (Fe), cobalt (Co), zinc (Zn) and cadmium (Cd) quotas, and the net use efficiencies (NUEs) of these bioactive trace metals (mol C fixed per mol cellular trace metal per day). Under B(12)-replete conditions, cells grown at high pCO(2) had lower Fe, Zn and Cd quotas, and used those trace metals more efficiently in comparison with cells grown at low pCO(2). At high pCO(2), B(12)-limited cells had ~50% lower specific growth and carbon fixation rates, and used Fe ~15-fold less efficiently, and Zn and Cd ~3-fold less efficiently, in comparison with B(12)-replete cells. The observed higher Fe, Zn and Cd NUE under high pCO(2)/B(12)-replete conditions are consistent with predicted downregulation of carbon-concentrating mechanisms. Co quotas of B(12)-replete cells were 5- to 14-fold higher in comparison with B(12)-limited cells, suggesting that >80% of cellular Co of B(12)-limited cells was likely from B(12). Our results demonstrate that CO(2) and vitamin B(12) interactively influence growth, carbon fixation, trace metal requirements and trace metal NUE of this diatom. This suggests the need to consider complex feedback interactions between multiple environmental factors for this biogeochemically critical group of phytoplankton in the last glacial maximum as well as the current and future changing ocean. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-01. |
format |
Dataset |
author |
King, Andrew L Sañudo-Wilhelmy, Sergio A Leblanc, Karine Hutchins, David A Fu, Feixue |
author_facet |
King, Andrew L Sañudo-Wilhelmy, Sergio A Leblanc, Karine Hutchins, David A Fu, Feixue |
author_sort |
King, Andrew L |
title |
CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_short |
CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_full |
CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_fullStr |
CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_full_unstemmed |
CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom |
title_sort |
co2 and vitamin b12 interactions determine bioactive trace metal requirements of a subarctic pacific diatom |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2011 |
url |
https://dx.doi.org/10.1594/pangaea.835475 https://doi.pangaea.de/10.1594/PANGAEA.835475 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Ocean acidification Subarctic |
genre_facet |
Ocean acidification Subarctic |
op_relation |
http://www.bco-dmo.org/dataset/3770 https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1038/ismej.2010.211 http://www.bco-dmo.org/dataset/3770 https://cran.r-project.org/package=seacarb |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.835475 https://doi.org/10.1038/ismej.2010.211 |
_version_ |
1766158826800676864 |
spelling |
ftdatacite:10.1594/pangaea.835475 2023-05-15T17:51:37+02:00 CO2 and vitamin B12 interactions determine bioactive trace metal requirements of a subarctic Pacific diatom King, Andrew L Sañudo-Wilhelmy, Sergio A Leblanc, Karine Hutchins, David A Fu, Feixue 2011 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.835475 https://doi.pangaea.de/10.1594/PANGAEA.835475 en eng PANGAEA - Data Publisher for Earth & Environmental Science http://www.bco-dmo.org/dataset/3770 https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1038/ismej.2010.211 http://www.bco-dmo.org/dataset/3770 https://cran.r-project.org/package=seacarb Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Attheya sp. Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Chromista Growth/Morphology Laboratory experiment Laboratory strains Micro-nutrients North Pacific Ochrophyta Pelagos Phytoplankton Primary production/Photosynthesis Single species Species Treatment Partial pressure of carbon dioxide water at sea surface temperature wet air Growth rate Growth rate, standard deviation Primary production Primary production of carbon, standard deviation Carbon/Phosphorus ratio Carbon/Phosphorus ratio, standard deviation Nitrogen/Phosphorus ratio Nitrogen/Phosphorus ratio, standard deviation Iron/Phosphorus ratio Iron/Phosphorus ratio, standard deviation Cobalt/Phosphorus ratio Cobalt/Phosphorus ratio, standard deviation Zinc/Phosphorus ratio Zinc/Phosphorus ratio, standard deviation Cadmium/Phosphorus ratio Cadmium/Phosphorus ratio, standard deviation Net use efficiency, Iron Net use efficiency, Iron, standard deviation Net use efficiency, Cobalt Net use efficiency, Cobalt, standard deviation Net use efficiency, Zinc Net use efficiency, Zinc, standard deviation Net use efficiency, Cadmium Net use efficiency, Cadmium, standard deviation Salinity Temperature, water pH pH, standard deviation Carbon, inorganic, dissolved Carbon, inorganic, dissolved, standard deviation Partial pressure of carbon dioxide, standard deviation Carbonate system computation flag Carbon dioxide Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Potentiometric Coulometric titration Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC dataset Dataset 2011 ftdatacite https://doi.org/10.1594/pangaea.835475 https://doi.org/10.1038/ismej.2010.211 2022-02-08T16:27:35Z Phytoplankton growth can be limited by numerous inorganic nutrients and organic growth factors. Using the subarctic diatom Attheya sp. in culture studies, we examined how the availability of vitamin B(12) and carbon dioxide partial pressure (pCO(2)) influences growth rate, primary productivity, cellular iron (Fe), cobalt (Co), zinc (Zn) and cadmium (Cd) quotas, and the net use efficiencies (NUEs) of these bioactive trace metals (mol C fixed per mol cellular trace metal per day). Under B(12)-replete conditions, cells grown at high pCO(2) had lower Fe, Zn and Cd quotas, and used those trace metals more efficiently in comparison with cells grown at low pCO(2). At high pCO(2), B(12)-limited cells had ~50% lower specific growth and carbon fixation rates, and used Fe ~15-fold less efficiently, and Zn and Cd ~3-fold less efficiently, in comparison with B(12)-replete cells. The observed higher Fe, Zn and Cd NUE under high pCO(2)/B(12)-replete conditions are consistent with predicted downregulation of carbon-concentrating mechanisms. Co quotas of B(12)-replete cells were 5- to 14-fold higher in comparison with B(12)-limited cells, suggesting that >80% of cellular Co of B(12)-limited cells was likely from B(12). Our results demonstrate that CO(2) and vitamin B(12) interactively influence growth, carbon fixation, trace metal requirements and trace metal NUE of this diatom. This suggests the need to consider complex feedback interactions between multiple environmental factors for this biogeochemically critical group of phytoplankton in the last glacial maximum as well as the current and future changing ocean. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-09-01. Dataset Ocean acidification Subarctic DataCite Metadata Store (German National Library of Science and Technology) Pacific |