Acclimatization of the crustose coralline alga Porolithon onkodes to variable pCO2, supplement to: Johnson, Maggie Dorothy; Moriarty, Vincent; Carpenter, Robert C (2014): Acclimatization of the Crustose Coralline Alga Porolithon onkodes to Variable pCO2. PLoS ONE, 9(2), e87678

• Main Authors: Johnson, Maggie Dorothy, Moriarty, Vincent, Carpenter, Robert C
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Benthos; Calcification/Dissolution; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Macroalgae; Plantae; Porolithon onkodes; Primary production/Photosynthesis; Respiration; Rhodophyta; Single species; South Pacific; Tropical; Species; Treatment; Calcification rate of calcium carbonate; Calcification rate, standard error; Gross photosynthesis rate, oxygen; Gross photosynthesis rate, oxygen, standard error; Respiration rate, oxygen; Respiration rate, oxygen, standard error; Net photosynthesis rate, oxygen; Net photosynthesis rate, oxygen, standard error; Temperature, water; Temperature, water, standard error; Salinity; Salinity, standard error; Irradiance; Irradiance, standard error; pH; pH, standard error; Alkalinity, total; Alkalinity, total, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Calcite saturation state; Calcite saturation state, standard error; Aragonite saturation state; Aragonite saturation state, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Spectrophotometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Moriarty; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.835310; https://doi.pangaea.de/10.1594/PANGAEA.835310

Ocean acidification (OA) has important implications for the persistence of coral reef ecosystems, due to potentially negative effects on biomineralization. Many coral reefs are dynamic with respect to carbonate chemistry, and experience fluctuations in pCO2 that exceed OA projections for the near future. To understand the influence of dynamic pCO2 on an important reef calcifier, we tested the response of the crustose coralline alga Porolithon onkodes to oscillating pCO2. Individuals were exposed to ambient (400 µatm), high (660 µatm), or variable pCO2 (oscillating between 400/660 µatm) treatments for 14 days. To explore the potential for coralline acclimatization, we collected individuals from low and high pCO2 variability sites (upstream and downstream respectively) on a back reef characterized by unidirectional water flow in Moorea, French Polynesia. We quantified the effects of treatment on algal calcification by measuring the change in buoyant weight, and on algal metabolism by conducting sealed incubations to measure rates of photosynthesis and respiration. Net photosynthesis was higher in the ambient treatment than the variable treatment, regardless of habitat origin, and there was no effect on respiration or gross photosynthesis. Exposure to high pCO2 decreased P. onkodes calcification by >70%, regardless of the original habitat. In the variable treatment, corallines from the high variability habitat calcified 42% more than corallines from the low variability habitat. The significance of the original habitat for the coralline calcification response to variable, high pCO2 indicates that individuals existing in dynamic pCO2 habitats may be acclimatized to OA within the scope of in situ variability. These results highlight the importance of accounting for natural pCO2 variability in OA manipulations, and provide insight into the potential for plasticity in habitat and species-specific responses to changing ocean chemistry. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-08-27.