Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ...
For the determination of water-soluble protein content of C. finmarchicus of the different stations the Qubit® Protein Assay Kit (Invitrogen) was used. Analysis was performed with extracts of 10 copepods. Working solution was prepared with Qubit® protein reagent and Qubit® protein buffer (1:200). 19...
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Online Access: | https://dx.doi.org/10.1594/pangaea.835180 https://doi.pangaea.de/10.1594/PANGAEA.835180 |
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ftdatacite:10.1594/pangaea.835180 2024-09-09T19:34:58+00:00 Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... Schmithüsen, Tosia Niehoff, Barbara Auel, Holger 2014 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.835180 https://doi.pangaea.de/10.1594/PANGAEA.835180 en eng PANGAEA https://dx.doi.org/10.1594/pangaea.835181 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 Event label Latitude of event Longitude of event Date/Time of event Sample ID Taxon/taxa Uniform resource locator/link to reference Life stage Number of individuals Proteins per individual Double opening/closing plankton net TriStar LB 941 Microplate Reader Berthold Technologies MSM26 Maria S. Merian Basin Scale Analysis, Synthesis and Integration EURO-BASIN Dataset dataset 2014 ftdatacite https://doi.org/10.1594/pangaea.83518010.1594/pangaea.835181 2024-07-03T13:11:36Z For the determination of water-soluble protein content of C. finmarchicus of the different stations the Qubit® Protein Assay Kit (Invitrogen) was used. Analysis was performed with extracts of 10 copepods. Working solution was prepared with Qubit® protein reagent and Qubit® protein buffer (1:200). 190 µL working solution was pipetted into each well of a micro plate and 10 µL of sample or Qubit® protein standard (0, 200 and 400 ng/µL) was added. Solutions were mixed and incubated for 15 min at room temperature. Measurements were conducted with a micro plate reader (TriStar LB 941, Berthold Technologies) at 485 nm excitation and 590 nm emission, using the software MikroWin2000 (Berthold Technologies). ... Dataset Calanus finmarchicus North Atlantic Copepods DataCite |
institution |
Open Polar |
collection |
DataCite |
op_collection_id |
ftdatacite |
language |
English |
topic |
Event label Latitude of event Longitude of event Date/Time of event Sample ID Taxon/taxa Uniform resource locator/link to reference Life stage Number of individuals Proteins per individual Double opening/closing plankton net TriStar LB 941 Microplate Reader Berthold Technologies MSM26 Maria S. Merian Basin Scale Analysis, Synthesis and Integration EURO-BASIN |
spellingShingle |
Event label Latitude of event Longitude of event Date/Time of event Sample ID Taxon/taxa Uniform resource locator/link to reference Life stage Number of individuals Proteins per individual Double opening/closing plankton net TriStar LB 941 Microplate Reader Berthold Technologies MSM26 Maria S. Merian Basin Scale Analysis, Synthesis and Integration EURO-BASIN Schmithüsen, Tosia Niehoff, Barbara Auel, Holger Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... |
topic_facet |
Event label Latitude of event Longitude of event Date/Time of event Sample ID Taxon/taxa Uniform resource locator/link to reference Life stage Number of individuals Proteins per individual Double opening/closing plankton net TriStar LB 941 Microplate Reader Berthold Technologies MSM26 Maria S. Merian Basin Scale Analysis, Synthesis and Integration EURO-BASIN |
description |
For the determination of water-soluble protein content of C. finmarchicus of the different stations the Qubit® Protein Assay Kit (Invitrogen) was used. Analysis was performed with extracts of 10 copepods. Working solution was prepared with Qubit® protein reagent and Qubit® protein buffer (1:200). 190 µL working solution was pipetted into each well of a micro plate and 10 µL of sample or Qubit® protein standard (0, 200 and 400 ng/µL) was added. Solutions were mixed and incubated for 15 min at room temperature. Measurements were conducted with a micro plate reader (TriStar LB 941, Berthold Technologies) at 485 nm excitation and 590 nm emission, using the software MikroWin2000 (Berthold Technologies). ... |
format |
Dataset |
author |
Schmithüsen, Tosia Niehoff, Barbara Auel, Holger |
author_facet |
Schmithüsen, Tosia Niehoff, Barbara Auel, Holger |
author_sort |
Schmithüsen, Tosia |
title |
Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... |
title_short |
Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... |
title_full |
Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... |
title_fullStr |
Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... |
title_full_unstemmed |
Protein content of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... |
title_sort |
protein content of calanus finmarchicus from the north atlantic collected during maria s. merian cruise msm26 in spring 2013 ... |
publisher |
PANGAEA |
publishDate |
2014 |
url |
https://dx.doi.org/10.1594/pangaea.835180 https://doi.pangaea.de/10.1594/PANGAEA.835180 |
genre |
Calanus finmarchicus North Atlantic Copepods |
genre_facet |
Calanus finmarchicus North Atlantic Copepods |
op_relation |
https://dx.doi.org/10.1594/pangaea.835181 |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_doi |
https://doi.org/10.1594/pangaea.83518010.1594/pangaea.835181 |
_version_ |
1809904393304145920 |