Enzyme activities of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ...
The activities of proteinases, lipases/esterases and citrate synthase of Calanus finmarchicus copepodites (CV) were analysed. Analysis was performed at 30°C for copepods from seven stations (126-9, 127-17, 131-17, 133-6, 134-19, 135-16, 136-8). In addition, thermal profiles (5-50°C) of these enzymes...
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ftdatacite:10.1594/pangaea.835177 2024-03-31T07:52:07+00:00 Enzyme activities of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... Schmithüsen, Tosia Niehoff, Barbara Auel, Holger 2014 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.835177 https://doi.pangaea.de/10.1594/PANGAEA.835177 en eng PANGAEA https://dx.doi.org/10.1594/pangaea.835181 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 Event label Latitude of event Longitude of event Date/Time of event DEPTH, water Depth, top/min Depth, bottom/max Sample ID Treatment Taxon/taxa Uniform resource locator/link to reference Life stage Treatment temperature Date Number of individuals Absorbance of sample Absorbance of control Proteinase activity per individual, Delta extinction at 366 nm Proteinase activity per dry mass, Delta extinction at 366 nm Lipase activity per individual Lipase activity, dry mass Citrate synthase activity per individual Citrate synthase activity, dry mass Double opening/closing plankton net WP-2 towed closing plankton net Spectrophotometer Thermo Scientific UV1 MSM26 Maria S. Merian Basin Scale Analysis, Synthesis and Integration EURO-BASIN dataset Dataset 2014 ftdatacite https://doi.org/10.1594/pangaea.83517710.1594/pangaea.835181 2024-03-04T14:01:54Z The activities of proteinases, lipases/esterases and citrate synthase of Calanus finmarchicus copepodites (CV) were analysed. Analysis was performed at 30°C for copepods from seven stations (126-9, 127-17, 131-17, 133-6, 134-19, 135-16, 136-8). In addition, thermal profiles (5-50°C) of these enzymes were analysed for copepods from 3 stations (127-17, 133-6, 135-16). C. finmarchicus of station 127-19 have been acclimated on board to two different temperatures (4 and 15°C) for two weeks. Thermal profiles (5-60°C) of lipases/esterases and proteinases of adult females from each treatment were analysed. Groups of 10 individuals were used to prepare enzyme extracts for analysis. From each station/treatment, three groups were analysed, each of which was measured in triplicates.The activity of proteinases was determined photometrically after Saborowski et al. (2004, hdl:10013/epic.20836), modified after Kreibich et al. (2008, doi:10.1007/s10152-008-0112-0). Azocasein was used as substrate. The lypolytic activity of ... Dataset Calanus finmarchicus North Atlantic Copepods DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Event label Latitude of event Longitude of event Date/Time of event DEPTH, water Depth, top/min Depth, bottom/max Sample ID Treatment Taxon/taxa Uniform resource locator/link to reference Life stage Treatment temperature Date Number of individuals Absorbance of sample Absorbance of control Proteinase activity per individual, Delta extinction at 366 nm Proteinase activity per dry mass, Delta extinction at 366 nm Lipase activity per individual Lipase activity, dry mass Citrate synthase activity per individual Citrate synthase activity, dry mass Double opening/closing plankton net WP-2 towed closing plankton net Spectrophotometer Thermo Scientific UV1 MSM26 Maria S. Merian Basin Scale Analysis, Synthesis and Integration EURO-BASIN |
spellingShingle |
Event label Latitude of event Longitude of event Date/Time of event DEPTH, water Depth, top/min Depth, bottom/max Sample ID Treatment Taxon/taxa Uniform resource locator/link to reference Life stage Treatment temperature Date Number of individuals Absorbance of sample Absorbance of control Proteinase activity per individual, Delta extinction at 366 nm Proteinase activity per dry mass, Delta extinction at 366 nm Lipase activity per individual Lipase activity, dry mass Citrate synthase activity per individual Citrate synthase activity, dry mass Double opening/closing plankton net WP-2 towed closing plankton net Spectrophotometer Thermo Scientific UV1 MSM26 Maria S. Merian Basin Scale Analysis, Synthesis and Integration EURO-BASIN Schmithüsen, Tosia Niehoff, Barbara Auel, Holger Enzyme activities of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... |
topic_facet |
Event label Latitude of event Longitude of event Date/Time of event DEPTH, water Depth, top/min Depth, bottom/max Sample ID Treatment Taxon/taxa Uniform resource locator/link to reference Life stage Treatment temperature Date Number of individuals Absorbance of sample Absorbance of control Proteinase activity per individual, Delta extinction at 366 nm Proteinase activity per dry mass, Delta extinction at 366 nm Lipase activity per individual Lipase activity, dry mass Citrate synthase activity per individual Citrate synthase activity, dry mass Double opening/closing plankton net WP-2 towed closing plankton net Spectrophotometer Thermo Scientific UV1 MSM26 Maria S. Merian Basin Scale Analysis, Synthesis and Integration EURO-BASIN |
description |
The activities of proteinases, lipases/esterases and citrate synthase of Calanus finmarchicus copepodites (CV) were analysed. Analysis was performed at 30°C for copepods from seven stations (126-9, 127-17, 131-17, 133-6, 134-19, 135-16, 136-8). In addition, thermal profiles (5-50°C) of these enzymes were analysed for copepods from 3 stations (127-17, 133-6, 135-16). C. finmarchicus of station 127-19 have been acclimated on board to two different temperatures (4 and 15°C) for two weeks. Thermal profiles (5-60°C) of lipases/esterases and proteinases of adult females from each treatment were analysed. Groups of 10 individuals were used to prepare enzyme extracts for analysis. From each station/treatment, three groups were analysed, each of which was measured in triplicates.The activity of proteinases was determined photometrically after Saborowski et al. (2004, hdl:10013/epic.20836), modified after Kreibich et al. (2008, doi:10.1007/s10152-008-0112-0). Azocasein was used as substrate. The lypolytic activity of ... |
format |
Dataset |
author |
Schmithüsen, Tosia Niehoff, Barbara Auel, Holger |
author_facet |
Schmithüsen, Tosia Niehoff, Barbara Auel, Holger |
author_sort |
Schmithüsen, Tosia |
title |
Enzyme activities of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... |
title_short |
Enzyme activities of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... |
title_full |
Enzyme activities of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... |
title_fullStr |
Enzyme activities of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... |
title_full_unstemmed |
Enzyme activities of Calanus finmarchicus from the North Atlantic collected during Maria S. Merian cruise MSM26 in spring 2013 ... |
title_sort |
enzyme activities of calanus finmarchicus from the north atlantic collected during maria s. merian cruise msm26 in spring 2013 ... |
publisher |
PANGAEA |
publishDate |
2014 |
url |
https://dx.doi.org/10.1594/pangaea.835177 https://doi.pangaea.de/10.1594/PANGAEA.835177 |
genre |
Calanus finmarchicus North Atlantic Copepods |
genre_facet |
Calanus finmarchicus North Atlantic Copepods |
op_relation |
https://dx.doi.org/10.1594/pangaea.835181 |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_doi |
https://doi.org/10.1594/pangaea.83517710.1594/pangaea.835181 |
_version_ |
1795031086351253504 |