The photo-physiological response of a model cnidarian-dinoflagellate symbiosis to CO2-induced acidification at the cellular level ...

• Main Authors: Gibbin, Emma M, Davy, Simon K
• Format: Dataset
• Language: English
• Published: PANGAEA 2014
• Subjects: Aiptasia pulchella; Animalia; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Cnidaria; Laboratory experiment; Laboratory strains; Primary production/Photosynthesis; Respiration; Single species; South Pacific; Species; pH, NBS scale; pH, standard error; Incubation duration; Symbiont cell density; Symbiont cell density, standard error; Maximum photochemical quantum yield of photosystem II; Maximum photochemical quantum yield of photosystem II, standard error; Gross photosynthesis rate, oxygen; Gross photosynthesis rate, oxygen, standard error; Gross photosynthesis rate, oxygen, per cell; Respiration rate, oxygen; Respiration rate, oxygen, standard error; Gross photosynthesis/respiration ratio; Gross photosynthesis/respiration ratio, standard error; pH, intracellular; pH, intracellular, standard error; Temperature, water; Temperature, water, standard error; Salinity; Alkalinity, total; Alkalinity, total, standard error; Bicarbonate ion; Bicarbonate ion, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Carbonate system computation flag; pH, total scale; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Potentiometric; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.835120; https://doi.pangaea.de/10.1594/PANGAEA.835120

We measured the relationship between CO2-induced seawater acidification, photo-physiological performance and intracellular pH (pHi) in a model cnidarian-dinoflagellate symbiosis - the sea anemone Aiptasia sp. -under ambient (289.94 ± 12.54 µatm), intermediate (687.40 ± 25.10 µatm) and high (1459.92 ± 65.51 µatm) CO2 conditions. These treatments represented current CO2 levels, in addition to CO2 stabilisation scenarios IV and VI provided by the Intergovernmental Panel on Climate Change (IPCC). Anemones were exposed to each treatment for two months and sampled at regular intervals. At each time-point we measured a series of physiological responses: maximum dark-adapted fluorescent yield of PSII (Fv/Fm), gross photosynthetic rate, respiration rate, symbiont population density, and light-adapted pHi of both the dinoflagellate symbiont and isolated host anemone cell. We observed increases in all but one photo-physiological parameter (Pgross:R ratio). At the cellular level, increases in light-adapted symbiont pHi ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-08-21. ...