Carbon uptake rate calculated for sea-ice core samples during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012
Net Primary Production was measured using the 14**C uptake method with minor modifications. Melted sea ice samples were spiked with 0.1µCi ml**-1 of 14**C labelled sodium bicarbonate (Moravek Biochemicals, Brea, USA) and distributed in 10 clear bottles (20 ml each). Subsequently they were incubated...
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PANGAEA - Data Publisher for Earth & Environmental Science
2014
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Online Access: | https://dx.doi.org/10.1594/pangaea.834215 https://doi.pangaea.de/10.1594/PANGAEA.834215 |
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ftdatacite:10.1594/pangaea.834215 2023-05-15T16:39:26+02:00 Carbon uptake rate calculated for sea-ice core samples during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012 Fernández-Méndez, Mar Peeken, Ilka Boetius, Antje 2014 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.834215 https://doi.pangaea.de/10.1594/PANGAEA.834215 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://dx.doi.org/10.1594/pangaea.834221 https://dx.doi.org/10.5194/bg-12-3525-2015 https://dx.doi.org/10.1594/pangaea.834084 Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Sample code/label Optional event label Gear Description Latitude of event Longitude of event Date/Time of event Date/time start Comment DEPTH, ice/snow Depth, top/min Depth, bottom/max Carbon uptake rate, fractionated Carbon uptake rate, fractionated, normalized to chlorophyll a Parameter Ice station Measured Calculated ARK-XXVII/3 Polarstern Assessment of bacterial life and matter cycling in deep-sea surface sediments ABYSS dataset Dataset 2014 ftdatacite https://doi.org/10.1594/pangaea.834215 https://doi.org/10.1594/pangaea.834221 https://doi.org/10.5194/bg-12-3525-2015 https://doi.org/10.1594/pangaea.834084 2022-02-08T16:24:46Z Net Primary Production was measured using the 14**C uptake method with minor modifications. Melted sea ice samples were spiked with 0.1µCi ml**-1 of 14**C labelled sodium bicarbonate (Moravek Biochemicals, Brea, USA) and distributed in 10 clear bottles (20 ml each). Subsequently they were incubated for 12 h at -1.3°C under different scalar irradiances (0-420 µmol photons m**-2 s**-1) measured with a spherical sensor (Spherical Micro Quantum Sensor US-SQS/L, Heinz Walz, Effeltrich, Germany). At the end of the incubation, samples were filtered onto 0.2 µm nitrocellulose filters and the particulate radioactive carbon uptake was determined by liquid scintillation counting using Filter count scintillation cocktail (Perkin Elmer, Waltham, USA). The carbon uptake values in the dark were subtracted from the carbon uptake values measured in the light incubations. Dissolved inorganic carbon (DIC) was measured for each sample using the flow injection system (Hall and Aller, 1992). The DIC concentration was taken into account to calculate the amount of labeled bicarbonate incorporated into the cell. Carbon fixation rates were normalized volumetrically and by chlorophyll a. Photosynthesis-irradiance curves (PI curves) were fitted using MATLAB® according to the equation proposed by Platt et al. (1980) including a photoinhibition parameter (beta) and providing the main photosynthetic parameters: maximum Chla normalized carbon fixation rate if there were no photoinhibition (Pb) and the initial slope of the saturation curve (alpha). The derived parameters: light intensity at which photosynthesis is maximal (Im), the carbon fixation rate at that maximal irradiance (Pbm) and the adaptation parameter or photoacclimation index (Ik) were calculated according to Platt et al. (1982). Dataset ice core Sea ice DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Sample code/label Optional event label Gear Description Latitude of event Longitude of event Date/Time of event Date/time start Comment DEPTH, ice/snow Depth, top/min Depth, bottom/max Carbon uptake rate, fractionated Carbon uptake rate, fractionated, normalized to chlorophyll a Parameter Ice station Measured Calculated ARK-XXVII/3 Polarstern Assessment of bacterial life and matter cycling in deep-sea surface sediments ABYSS |
spellingShingle |
Sample code/label Optional event label Gear Description Latitude of event Longitude of event Date/Time of event Date/time start Comment DEPTH, ice/snow Depth, top/min Depth, bottom/max Carbon uptake rate, fractionated Carbon uptake rate, fractionated, normalized to chlorophyll a Parameter Ice station Measured Calculated ARK-XXVII/3 Polarstern Assessment of bacterial life and matter cycling in deep-sea surface sediments ABYSS Fernández-Méndez, Mar Peeken, Ilka Boetius, Antje Carbon uptake rate calculated for sea-ice core samples during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012 |
topic_facet |
Sample code/label Optional event label Gear Description Latitude of event Longitude of event Date/Time of event Date/time start Comment DEPTH, ice/snow Depth, top/min Depth, bottom/max Carbon uptake rate, fractionated Carbon uptake rate, fractionated, normalized to chlorophyll a Parameter Ice station Measured Calculated ARK-XXVII/3 Polarstern Assessment of bacterial life and matter cycling in deep-sea surface sediments ABYSS |
description |
Net Primary Production was measured using the 14**C uptake method with minor modifications. Melted sea ice samples were spiked with 0.1µCi ml**-1 of 14**C labelled sodium bicarbonate (Moravek Biochemicals, Brea, USA) and distributed in 10 clear bottles (20 ml each). Subsequently they were incubated for 12 h at -1.3°C under different scalar irradiances (0-420 µmol photons m**-2 s**-1) measured with a spherical sensor (Spherical Micro Quantum Sensor US-SQS/L, Heinz Walz, Effeltrich, Germany). At the end of the incubation, samples were filtered onto 0.2 µm nitrocellulose filters and the particulate radioactive carbon uptake was determined by liquid scintillation counting using Filter count scintillation cocktail (Perkin Elmer, Waltham, USA). The carbon uptake values in the dark were subtracted from the carbon uptake values measured in the light incubations. Dissolved inorganic carbon (DIC) was measured for each sample using the flow injection system (Hall and Aller, 1992). The DIC concentration was taken into account to calculate the amount of labeled bicarbonate incorporated into the cell. Carbon fixation rates were normalized volumetrically and by chlorophyll a. Photosynthesis-irradiance curves (PI curves) were fitted using MATLAB® according to the equation proposed by Platt et al. (1980) including a photoinhibition parameter (beta) and providing the main photosynthetic parameters: maximum Chla normalized carbon fixation rate if there were no photoinhibition (Pb) and the initial slope of the saturation curve (alpha). The derived parameters: light intensity at which photosynthesis is maximal (Im), the carbon fixation rate at that maximal irradiance (Pbm) and the adaptation parameter or photoacclimation index (Ik) were calculated according to Platt et al. (1982). |
format |
Dataset |
author |
Fernández-Méndez, Mar Peeken, Ilka Boetius, Antje |
author_facet |
Fernández-Méndez, Mar Peeken, Ilka Boetius, Antje |
author_sort |
Fernández-Méndez, Mar |
title |
Carbon uptake rate calculated for sea-ice core samples during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012 |
title_short |
Carbon uptake rate calculated for sea-ice core samples during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012 |
title_full |
Carbon uptake rate calculated for sea-ice core samples during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012 |
title_fullStr |
Carbon uptake rate calculated for sea-ice core samples during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012 |
title_full_unstemmed |
Carbon uptake rate calculated for sea-ice core samples during POLARSTERN cruise ARK-XXVII/3 (IceArc) in 2012 |
title_sort |
carbon uptake rate calculated for sea-ice core samples during polarstern cruise ark-xxvii/3 (icearc) in 2012 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2014 |
url |
https://dx.doi.org/10.1594/pangaea.834215 https://doi.pangaea.de/10.1594/PANGAEA.834215 |
genre |
ice core Sea ice |
genre_facet |
ice core Sea ice |
op_relation |
https://dx.doi.org/10.1594/pangaea.834221 https://dx.doi.org/10.5194/bg-12-3525-2015 https://dx.doi.org/10.1594/pangaea.834084 |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.834215 https://doi.org/10.1594/pangaea.834221 https://doi.org/10.5194/bg-12-3525-2015 https://doi.org/10.1594/pangaea.834084 |
_version_ |
1766029774129463296 |