Impacts of groundwater discharge at Myora Springs (North Stradbroke Island, Australia) on the phenolic metabolism of eelgrass, Zostera muelleri, and grazing by the juvenile rabbitfish, Siganus fuscescens, supplement to: Arnold, Thomas; Freundlich, Grace; Weilnau, Taylor; Verdi, Arielle; Tibbetts, Ian R (2014): Impacts of groundwater discharge at Myora Springs (North Stradbroke Island, Australia) on the phenolic metabolism of eelgrass, Zostera muelleri, and grazing by the juvenile rabbitfish, Siganus fuscescens. PLoS ONE, 9(8), e104738

• Main Authors: Arnold, Thomas, Freundlich, Grace, Weilnau, Taylor, Verdi, Arielle, Tibbetts, Ian R
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Benthos; Biomass/Abundance/Elemental composition; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Cymodocea nodosa; Field experiment; Macroalgae; Other metabolic rates; Plantae; Ruppia maritima; Single species; South Pacific; Temperate; Tracheophyta; Zostera muelleri; Event label; Species; Distance; Identification; Location; δ13C; δ15N; Carbon, total; Nitrogen, total; Carbon/Nitrogen ratio; Phenolics, reactive, total; Proanthocyanidins; Gallic acid; Rosmarinic acid; Caffeic acid; Lignin; Protein; Starch; Sugars; Salinity; Salinity, standard error; Temperature, water; Temperature, water, standard error; pH; pH, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Alkalinity, total; Alkalinity, total, standard error; Nitrogen, standard error; Nitrogen in nitrate; Nitrogen in nitrate, standard error; Nitrogen in nitrite + nitrate; Nitrogen in nitrite + nitrate, standard error; Nitrogen in nitrite; Nitrogen in nitrite, standard error; Sulfate; Sulfate, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Carbon, inorganic, dissolved; Aragonite saturation state; Calcite saturation state; Experiment; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Queensland; Moreton; Moreton Bay; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.834082; https://doi.pangaea.de/10.1594/PANGAEA.834082

Myora Springs is one of many groundwater discharge sites on North Stradbroke Island (Queensland, Australia). Here spring waters emerge from wetland forests to join Moreton Bay, mixing with seawater over seagrass meadows dominated by eelgrass, Zostera muelleri. We sought to determine how low pH / high CO2 conditions near the spring affect these plants and their interactions with the black rabbitfish (Siganus fuscescens), a co-occurring grazer. In paired-choice feeding trials S. fuscescens preferentially consumed Z. muelleri shoots collected nearest to Myora Springs. Proximity to the spring did not significantly alter the carbon and nitrogen contents of seagrass tissues but did result in the extraordinary loss of soluble phenolics, including Folin-reactive phenolics, condensed tannins, and phenolic acids by ?87%. Conversely, seagrass lignin contents were, in this and related experiments, unaffected or increased, suggesting a shift in secondary metabolism away from the production of soluble, but not insoluble, (poly)phenolics. We suggest that groundwater discharge sites such as Myora Springs, and other sites characterized by low pH, are likely to be popular feeding grounds for seagrass grazers seeking to reduce their exposure to soluble phenolics. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-15.