Acid-base regulation in the Dungeness crab (Metacarcinus magister), supplement to: Hans, Stephanie; Fehsenfeld, Sandra; Treberg, Jason R; Weihrauch, Dirk (2014): Acid–base regulation in the Dungeness crab (Metacarcinus magister). Marine Biology, 161(5), 1179-1193

• Main Authors: Hans, Stephanie, Fehsenfeld, Sandra, Treberg, Jason R, Weihrauch, Dirk
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Acid-base regulation; Animalia; Arthropoda; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Metacarcinus magister; Not applicable; Other metabolic rates; Respiration; Single species; Temperate; Species; Figure; Table; Treatment; Identification; Haemolymph, pH; Haemolymph, partial pressure of carbon dioxide; Haemolymph, bicarbonate ion; Ammonia excretion; Time point, descriptive; Respiration rate, oxygen; Replicates; pH; Sample code/label; Partial pressure of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion increase rate; Hemocyanin; Ammonia+Ammonium; Sodium ion; Potassium ion; Calcium ion; Magnesium ion; Chloride ion; Sulfate ion; Osmolality; Salinity; Temperature, water; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Bicarbonate ion; Bicarbonate ion, standard error; Alkalinity, total; Alkalinity, total, standard error; pH, standard error; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Carbonate ion; Aragonite saturation state; Calcite saturation state; Coulometric titration; Calculated using CO2SYS; Potentiometric; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.833716; https://doi.pangaea.de/10.1594/PANGAEA.833716

Homeostatic regulation allows organisms to secure basic physiological processes in a varying environment. To counteract fluctuations in ambient carbonate system speciation due to elevated seawater pCO2 (hypercapnia), many aquatic crustaceans excrete/accumulate acid-base equivalents through their gills; however, not much is known about the role of ammonia in this response. The present study investigated the effects of hypercapnia on acid-base and ammonia regulation in the Dungeness crab, Metacarcinus magister on the whole animal and isolated gill levels. Hemolymph pCO2 and [HCO3]- increased in M. magister acclimated to elevated pCO2 (330 Pa), while pH remained stable. Additionally, hemolymph [Na+], [Ca2+], and [SO4]2- were significantly increased. When challenged with varying pH during gill perfusion, the pH of the artificial hemolymph remained relatively unchanged. Overall, ammonia production and excretion, as well as oxygen consumption, were reduced in crabs acclimated to elevated pCO2, demonstrating that either (amino acid) oxidation is reduced in response to this particular stress, or nitrogenous wastes are excreted in an alternative form. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-07-01.