CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic, supplement to: Bögner, Desislava; Bickmeyer, Ulf; Köhler, Angela (2014): CO2-induced fertilization impairment in Strongylocentrotus droebachiensis collected in the Arctic. Helgoland Marine Research, 68(2), 341-356

• Main Authors: Bögner, Desislava, Bickmeyer, Ulf, Köhler, Angela
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Animalia; Arctic; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Echinodermata; Laboratory experiment; Polar; Reproduction; FOS Medical biotechnology; Single species; Strongylocentrotus droebachiensis; Species; Experiment; Replicate; Time in hours; Treatment; Stage; Comment; Eggs; Egg stages; Relative fluorescence intensity, ratio; Salinity; Salinity, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Temperature, water; Temperature, water, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Mediterranean stages; Potentiometric titration; Potentiometric; Coulometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Helgoland; Ocean acidification; Spitsbergen
• Online Access: https://dx.doi.org/10.1594/pangaea.833621; https://doi.pangaea.de/10.1594/PANGAEA.833621

Fertilization depends on distribution and aggregation patterns of sea urchins which influence gamete contact time and may potentially enhance their vulnerability to ocean acidification. In this study, we conducted fertilization experiments to assess the effects of selected pH scenarios on fertilization success of Strongylocentrotus droebachiensis, from Spitsbergen, Arctic. Acidification was achieved by aerating seawater with different CO2 partial pressures to represent pre-industrial and present conditions (measured ~180-425 µatm) and future acidification scenarios (~550-800, ~1,300, ~2,000 µatm). Fertilization success was defined as the proportion of successful/unsuccessful fertilizations per treatment; eggs were classified according to features of their fertilization envelope (FE), hyaline layer (HL) and achievement of cellular division. The diagnostic findings of specific pathological aberrations were described in detail. We additionally measured intracellular pH changes in unfertilized eggs exposed for 1 h to selected acidification treatments using BCECF/AM. We conclude that (a) acidified conditions increase the proportion of eggs that failed fertilization, (b) acidification may increase the risk of polyspermy due to failures in the FE formation supported by the occasional observation of multiple sperms in the perivitelline space and (c) irregular formation of the embryo may arise due to impaired formation of the HL. The decrease in fertilization success could be also related to the observed changes in intracellular pH at pCO2 ~ 1,000 µatm or higher. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-06-19.