Effects of pre-hatching pCO2 on development and survival of zoea stages of Arctic spider crab Hyas araneus, supplement to: Schiffer, Melanie; Harms, Lars; Pörtner, Hans-Otto; Mark, Felix Christopher; Storch, Daniela (2014): Pre-hatching seawater pCO2 affects development and survival of zoea stages of Arctic spider crab Hyas araneus. Marine Ecology Progress Series, 501, 127-139

• Main Authors: Schiffer, Melanie, Harms, Lars, Pörtner, Hans-Otto, Mark, Felix Christopher, Storch, Daniela
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2014
• Subjects: Animalia; Arctic; Arthropoda; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Development; Growth/Morphology; Hyas araneus; Laboratory experiment; Mortality/Survival; Pelagos; Polar; Respiration; Single species; Zooplankton; Species; Figure; Partial pressure of carbon dioxide water at sea surface temperature wet air; Incubation duration; Larvae; Larvae, dead; Respiration rate, oxygen, per individual; Dry mass per individual; Heart beat rate; Maxilliped beat rate; Stage; Temperature, water; Feeding rate per individual; Duration, number of days; Temperature, water, standard deviation; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide, standard deviation; Salinity; Salinity, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Alkalinity, total; Aragonite saturation state; Calcite saturation state; Experiment; Potentiometric; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.833067; https://doi.pangaea.de/10.1594/PANGAEA.833067

Sensitivity of marine crustaceans to anthropogenic CO2 emissions and the associated acidification of the oceans may be less than that of other, especially lower, invertebrates. However, effects on critical transition phases or carry-over effects between life stages have not comprehensively been explored. Here we report the impact of elevated seawater PCO2 values (3100 µatm) on Hyas araneus during the last 2 weeks of their embryonic development (pre-hatching phase) and during development while in the consecutive zoea I and zoea II larval stages (post-hatching phase). We measured oxygen consumption, dry weight, developmental time and mortality in zoea I to assess changes in performance. Feeding rates and survival under starvation were investigated at different temperatures to detect differences in thermal sensitivities of zoea I and zoea II larvae depending on pre-hatch history. When embryos were pre-exposed to elevated PCO2 during maternal care, mortality increased about 60% under continued CO2 exposure during the zoea I phase. The larvae that moulted into zoea II, displayed a developmental delay by about 20 days compared to larvae exposed to control PCO2 during embryonic and zoeal phases. Elevated PCO2 caused a reduction in zoea I dry weight and feeding rates, while survival of the starved larvae was not affected by the seawater CO2 concentration. In conclusion, CO2 effects on egg masses under maternal care carried over to the first larval stages of crustaceans and reduced their survival and development to levels below those previously reported in studies exclusively focussing on acute PCO2 effects on the larval stages. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-05-29.