Expression of biomineralization-related ion transport genes in Emiliania huxleyi ...
Biomineralization in the marine phytoplankton Emiliania huxleyi is a stringently controlled intracellular process. The molecular basis of coccolith production is still relatively unknown although its importance in global biogeochemical cycles and varying sensitivity to increased pCO2 levels has been...
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ftdatacite:10.1594/pangaea.832450 2023-11-05T03:44:27+01:00 Expression of biomineralization-related ion transport genes in Emiliania huxleyi ... Mackinder, Luke C M Wheeler, Glen Schroeder, Declan C von Dassow, Peter Riebesell, Ulf Brownlee, Colin 2011 application/zip https://dx.doi.org/10.1594/pangaea.832450 https://doi.pangaea.de/10.1594/PANGAEA.832450 en eng PANGAEA https://dx.doi.org/10.1111/j.1462-2920.2011.02561.x Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 Biological Impacts of Ocean Acidification BIOACID article Supplementary Publication Series of Datasets Collection 2011 ftdatacite https://doi.org/10.1594/pangaea.83245010.1111/j.1462-2920.2011.02561.x 2023-10-09T10:16:07Z Biomineralization in the marine phytoplankton Emiliania huxleyi is a stringently controlled intracellular process. The molecular basis of coccolith production is still relatively unknown although its importance in global biogeochemical cycles and varying sensitivity to increased pCO2 levels has been well documented. This study looks into the role of several candidate Ca2+, H+ and inorganic carbon transport genes in E. huxleyi, using quantitative reverse transcriptase PCR. Differential gene expression analysis was investigated in two isogenic pairs of calcifying and non-calcifying strains of E. huxleyi and cultures grown at various Ca2+ concentrations to alter calcite production. We show that calcification correlated to the consistent upregulation of a putative HCO3- transporter belonging to the solute carrier 4 (SLC4) family, a Ca2+/H+ exchanger belonging to the CAX family of exchangers and a vacuolar H+-ATPase. We also show that the coccolith-associated protein, GPA is downregulated in calcifying cells. The ... : Supplement to: Mackinder, Luke C M; Wheeler, Glen; Schroeder, Declan C; von Dassow, Peter; Riebesell, Ulf; Brownlee, Colin (2011): Expression of biomineralization-related ion transport genes in Emiliania huxleyi. Environmental Microbiology, 13(12), 3250-3265 ... Article in Journal/Newspaper Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
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Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
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ftdatacite |
language |
English |
topic |
Biological Impacts of Ocean Acidification BIOACID |
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Biological Impacts of Ocean Acidification BIOACID Mackinder, Luke C M Wheeler, Glen Schroeder, Declan C von Dassow, Peter Riebesell, Ulf Brownlee, Colin Expression of biomineralization-related ion transport genes in Emiliania huxleyi ... |
topic_facet |
Biological Impacts of Ocean Acidification BIOACID |
description |
Biomineralization in the marine phytoplankton Emiliania huxleyi is a stringently controlled intracellular process. The molecular basis of coccolith production is still relatively unknown although its importance in global biogeochemical cycles and varying sensitivity to increased pCO2 levels has been well documented. This study looks into the role of several candidate Ca2+, H+ and inorganic carbon transport genes in E. huxleyi, using quantitative reverse transcriptase PCR. Differential gene expression analysis was investigated in two isogenic pairs of calcifying and non-calcifying strains of E. huxleyi and cultures grown at various Ca2+ concentrations to alter calcite production. We show that calcification correlated to the consistent upregulation of a putative HCO3- transporter belonging to the solute carrier 4 (SLC4) family, a Ca2+/H+ exchanger belonging to the CAX family of exchangers and a vacuolar H+-ATPase. We also show that the coccolith-associated protein, GPA is downregulated in calcifying cells. The ... : Supplement to: Mackinder, Luke C M; Wheeler, Glen; Schroeder, Declan C; von Dassow, Peter; Riebesell, Ulf; Brownlee, Colin (2011): Expression of biomineralization-related ion transport genes in Emiliania huxleyi. Environmental Microbiology, 13(12), 3250-3265 ... |
format |
Article in Journal/Newspaper |
author |
Mackinder, Luke C M Wheeler, Glen Schroeder, Declan C von Dassow, Peter Riebesell, Ulf Brownlee, Colin |
author_facet |
Mackinder, Luke C M Wheeler, Glen Schroeder, Declan C von Dassow, Peter Riebesell, Ulf Brownlee, Colin |
author_sort |
Mackinder, Luke C M |
title |
Expression of biomineralization-related ion transport genes in Emiliania huxleyi ... |
title_short |
Expression of biomineralization-related ion transport genes in Emiliania huxleyi ... |
title_full |
Expression of biomineralization-related ion transport genes in Emiliania huxleyi ... |
title_fullStr |
Expression of biomineralization-related ion transport genes in Emiliania huxleyi ... |
title_full_unstemmed |
Expression of biomineralization-related ion transport genes in Emiliania huxleyi ... |
title_sort |
expression of biomineralization-related ion transport genes in emiliania huxleyi ... |
publisher |
PANGAEA |
publishDate |
2011 |
url |
https://dx.doi.org/10.1594/pangaea.832450 https://doi.pangaea.de/10.1594/PANGAEA.832450 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://dx.doi.org/10.1111/j.1462-2920.2011.02561.x |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_doi |
https://doi.org/10.1594/pangaea.83245010.1111/j.1462-2920.2011.02561.x |
_version_ |
1781704386671542272 |