Ocean acidification induces multi-generational decline in copepod naupliar production with possible conflict for reproductive resource allocation, supplement to: Fitzer, Susan C; Caldwell, Gary S; Close, Andrew J; Clare, Anthony S; Upstill-Goddard, Robert C; Bentley, Matthew G (2012): Ocean acidification induces multi-generational decline in copepod naupliar production with possible conflict for reproductive resource allocation. Journal of Experimental Marine Biology and Ecology, 418-419, 30-36

• Main Authors: Fitzer, Susan C, Caldwell, Gary S, Close, Andrew J, Clare, Anthony S, Upstill-Goddard, Robert C, Bentley, Matthew G
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2012
• Subjects: Animalia; Arthropoda; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Growth/Morphology; Laboratory experiment; North Atlantic; Pelagos; Reproduction; FOS Medical biotechnology; Single species; Temperate; Tisbe battagliai; Zooplankton; Species; Treatment; Stage; Length; Sample code/label; Generation; Group; Nauplii; Replicates; Identification; Elements; Percentage; pH; pH, standard deviation; Temperature, water; Temperature, water, standard deviation; Salinity; Alkalinity, total; Bicarbonate ion; Carbonate ion; Partial pressure of carbon dioxide water at sea surface temperature wet air; Calcite saturation state; Aragonite saturation state; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Carbon, inorganic, dissolved; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Caldwell; Ocean acidification; Copepods
• Online Access: https://dx.doi.org/10.1594/pangaea.831728; https://doi.pangaea.de/10.1594/PANGAEA.831728

Climate change, including ocean acidification (OA), presents fundamental challenges to marine biodiversity and sustained ecosystem health. We determined reproductive response (measured as naupliar production), cuticle composition and stage specific growth of the copepod Tisbe battagliai over three generations at four pH conditions (pH 7.67, 7.82, 7.95, and 8.06). Naupliar production increased significantly at pH 7.95 compared with pH 8.06 followed by a decline at pH 7.82. Naupliar production at pH 7.67 was higher than pH 7.82. We attribute the increase at pH 7.95 to an initial stress response which was succeeded by a hormesis-like response at pH 7.67. A multi-generational modelling approach predicted a gradual decline in naupliar production over the next 100 years (equivalent to approximately 2430 generations). There was a significant growth reduction (mean length integrated across developmental stage) relative to controls. There was a significant increase in the proportion of carbon relative to oxygen within the cuticle as seawater pH decreased. Changes in growth, cuticle composition and naupliar production strongly suggest that copepods subjected to OA-induced stress preferentially reallocate resources towards maintaining reproductive output at the expense of somatic growth and cuticle composition. These responses may drive shifts in life history strategies that favour smaller brood sizes, females and perhaps later maturing females, with the potential to profoundly destabilise marine trophodynamics. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-04-11.