Photosynthate translocation increases in response to low seawater pH in a coral-dinoflagellate symbiosis, supplement to: Tremblay, Pascale; Fine, M; Maguer, Jean-François; Grover, Renaud; Ferrier-Pagès, Christine (2013): Photosynthate translocation increases in response to low seawater pH in a coral–dinoflagellate symbiosis. Biogeosciences, 10(6), 3997-4007

• Main Authors: Tremblay, Pascale, Fine, M, Maguer, Jean-François, Grover, Renaud, Ferrier-Pagès, Christine
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2013
• Subjects: Animalia; Benthic animals; Benthos; Cnidaria; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Laboratory experiment; Primary production/Photosynthesis; Red Sea; Respiration; Single species; Stylophora pistillata; Temperate; Species; Sample ID; pH; Cell density; Chlorophyll a; Chlorophyll c2; Protein; Gross photosynthesis rate, carbon dioxide; Respiration rate, carbon dioxide; Carbon, incorporated; Percentage; Carbon, lost; Carbon, translocated; Temperature, water; Salinity; Alkalinity, total; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbon dioxide; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Calcite saturation state; Potentiometric; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Renaud; Tremblay; Ferrier; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.831725; https://doi.pangaea.de/10.1594/PANGAEA.831725

This study has examined the effect of low seawater pH values (induced by an increased CO2 partial pressure) on the rates of photosynthesis, as well as on the carbon budget and carbon translocation in the scleractinian coral species Stylophora pistillata, using a new model based on 13C labelling of the photosynthetic products. Symbiont photosynthesis contributes to a large part of the carbon acquisition in tropical coral species, and it is thus important to know how environmental changes affect this carbon acquisition and allocation. For this purpose, nubbins of S. pistillata were maintained for six months at two pHTs (8.1 and 7.2, by bubbling seawater with CO2). The lowest pH value was used to tackle how seawater pH impacts the carbon budget of a scleractinian coral. Rates of photosynthesis and respiration of the symbiotic association and of isolated symbionts were assessed at each pH. The fate of 13C photosynthates was then followed in the symbionts and the coral host for 48 h. Nubbins maintained at pHT 7.2 presented a lower areal symbiont concentration, and lower areal rates of gross photosynthesis and carbon incorporation compared to nubbins maintained at pHT 8.1. The total carbon acquisition was thus lower under low pH. However, the total percentage of carbon translocated to the host as well as the amount of carbon translocated per symbiont cell were significantly higher under pHT 7.2 than under pHT 8.1 (70% at pHT 7.2 vs. 60% at pHT 8.1), such that the total amount of photosynthetic carbon received by the coral host was equivalent under both pHs (5.5 to 6.1 µg C/cm**2/h). Although the carbon budget of the host was unchanged, symbionts acquired less carbon for their own needs (0.6 compared to 1.8 µg C/cm**2/h), explaining the overall decrease in symbiont concentration at low pH. In the long term, such decrease in symbiont concentration might severely affect the carbon budget of the symbiotic association. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-04-11.