Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis, supplement to: Dineshram, R; Thiyagarajan, Vengatesen; Lane, Ackley Charles; Yu, Ziniu; Shu, Xiao; Leung, Priscilla TY (2013): Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis. Marine Biology, 160(8), 2189-2205
Ocean acidification (OA) is beginning to have noticeable negative impact on calcification rate, shell structure and physiological energy budgeting of several marine organisms; these alter the growth of many economically important shellfish including oysters. Early life stages of oysters may be parti...
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Format: | Dataset |
Language: | English |
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PANGAEA - Data Publisher for Earth & Environmental Science
2013
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Subjects: | |
Online Access: | https://dx.doi.org/10.1594/pangaea.831445 https://doi.pangaea.de/10.1594/PANGAEA.831445 |
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ftdatacite:10.1594/pangaea.831445 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Animalia Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Crassostrea hongkongensis Gene expression incl. proteomics Growth/Morphology Laboratory experiment Mollusca North Pacific Pelagos Single species Tropical Zooplankton Species Figure Treatment Replicates Incubation duration Shell length Growth rate Growth rate, standard deviation Protein name Protein spots, total Protein spots, total, standard deviation Spot intensity, relative Spot intensity, relative, standard deviation Temperature, water Salinity Alkalinity, total pH Carbon, inorganic, dissolved Partial pressure of carbon dioxide water at sea surface temperature wet air Carbon dioxide Bicarbonate ion Carbonate ion Calcite saturation state Aragonite saturation state Carbonate system computation flag Fugacity of carbon dioxide water at sea surface temperature wet air Potentiometric titration Potentiometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Animalia Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Crassostrea hongkongensis Gene expression incl. proteomics Growth/Morphology Laboratory experiment Mollusca North Pacific Pelagos Single species Tropical Zooplankton Species Figure Treatment Replicates Incubation duration Shell length Growth rate Growth rate, standard deviation Protein name Protein spots, total Protein spots, total, standard deviation Spot intensity, relative Spot intensity, relative, standard deviation Temperature, water Salinity Alkalinity, total pH Carbon, inorganic, dissolved Partial pressure of carbon dioxide water at sea surface temperature wet air Carbon dioxide Bicarbonate ion Carbonate ion Calcite saturation state Aragonite saturation state Carbonate system computation flag Fugacity of carbon dioxide water at sea surface temperature wet air Potentiometric titration Potentiometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Dineshram, R Thiyagarajan, Vengatesen Lane, Ackley Charles Yu, Ziniu Shu, Xiao Leung, Priscilla TY Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis, supplement to: Dineshram, R; Thiyagarajan, Vengatesen; Lane, Ackley Charles; Yu, Ziniu; Shu, Xiao; Leung, Priscilla TY (2013): Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis. Marine Biology, 160(8), 2189-2205 |
topic_facet |
Animalia Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Crassostrea hongkongensis Gene expression incl. proteomics Growth/Morphology Laboratory experiment Mollusca North Pacific Pelagos Single species Tropical Zooplankton Species Figure Treatment Replicates Incubation duration Shell length Growth rate Growth rate, standard deviation Protein name Protein spots, total Protein spots, total, standard deviation Spot intensity, relative Spot intensity, relative, standard deviation Temperature, water Salinity Alkalinity, total pH Carbon, inorganic, dissolved Partial pressure of carbon dioxide water at sea surface temperature wet air Carbon dioxide Bicarbonate ion Carbonate ion Calcite saturation state Aragonite saturation state Carbonate system computation flag Fugacity of carbon dioxide water at sea surface temperature wet air Potentiometric titration Potentiometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
description |
Ocean acidification (OA) is beginning to have noticeable negative impact on calcification rate, shell structure and physiological energy budgeting of several marine organisms; these alter the growth of many economically important shellfish including oysters. Early life stages of oysters may be particularly vulnerable to OA-driven low pH conditions because their shell is made up of the highly soluble form of calcium carbonate (CaCO3) mineral, aragonite. Our long-term CO2 perturbation experiment showed that larval shell growth rate of the oyster species Crassostrea hongkongensis was significantly reduced at pH < 7.9 compared to the control (8.2). To gain new insights into the underlying mechanisms of low-pH-induced delays in larval growth, we have examined the effect of pH on the protein expression pattern, including protein phosphorylation status at the pediveliger larval stage. Using two-dimensional electrophoresis and mass spectrometry, we demonstrated that the larval proteome was significantly altered by the two low pH treatments (7.9 and 7.6) compared to the control pH (8.2). Generally, the number of expressed proteins and their phosphorylation level decreased with low pH. Proteins involved in larval energy metabolism and calcification appeared to be down-regulated in response to low pH, whereas cell motility and production of cytoskeletal proteins were increased. This study on larval growth coupled with proteome change is the first step toward the search for novel Protein Expression Signatures indicative of low pH, which may help in understanding the mechanisms involved in low pH tolerance. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-04-03. |
format |
Dataset |
author |
Dineshram, R Thiyagarajan, Vengatesen Lane, Ackley Charles Yu, Ziniu Shu, Xiao Leung, Priscilla TY |
author_facet |
Dineshram, R Thiyagarajan, Vengatesen Lane, Ackley Charles Yu, Ziniu Shu, Xiao Leung, Priscilla TY |
author_sort |
Dineshram, R |
title |
Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis, supplement to: Dineshram, R; Thiyagarajan, Vengatesen; Lane, Ackley Charles; Yu, Ziniu; Shu, Xiao; Leung, Priscilla TY (2013): Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis. Marine Biology, 160(8), 2189-2205 |
title_short |
Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis, supplement to: Dineshram, R; Thiyagarajan, Vengatesen; Lane, Ackley Charles; Yu, Ziniu; Shu, Xiao; Leung, Priscilla TY (2013): Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis. Marine Biology, 160(8), 2189-2205 |
title_full |
Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis, supplement to: Dineshram, R; Thiyagarajan, Vengatesen; Lane, Ackley Charles; Yu, Ziniu; Shu, Xiao; Leung, Priscilla TY (2013): Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis. Marine Biology, 160(8), 2189-2205 |
title_fullStr |
Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis, supplement to: Dineshram, R; Thiyagarajan, Vengatesen; Lane, Ackley Charles; Yu, Ziniu; Shu, Xiao; Leung, Priscilla TY (2013): Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis. Marine Biology, 160(8), 2189-2205 |
title_full_unstemmed |
Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis, supplement to: Dineshram, R; Thiyagarajan, Vengatesen; Lane, Ackley Charles; Yu, Ziniu; Shu, Xiao; Leung, Priscilla TY (2013): Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis. Marine Biology, 160(8), 2189-2205 |
title_sort |
elevated co2 alters larval proteome and its phosphorylation status in the commercial oyster, crassostrea hongkongensis, supplement to: dineshram, r; thiyagarajan, vengatesen; lane, ackley charles; yu, ziniu; shu, xiao; leung, priscilla ty (2013): elevated co2 alters larval proteome and its phosphorylation status in the commercial oyster, crassostrea hongkongensis. marine biology, 160(8), 2189-2205 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2013 |
url |
https://dx.doi.org/10.1594/pangaea.831445 https://doi.pangaea.de/10.1594/PANGAEA.831445 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1007/s00227-013-2176-x https://cran.r-project.org/package=seacarb |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.831445 https://doi.org/10.1007/s00227-013-2176-x |
_version_ |
1766157845370241024 |
spelling |
ftdatacite:10.1594/pangaea.831445 2023-05-15T17:50:54+02:00 Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis, supplement to: Dineshram, R; Thiyagarajan, Vengatesen; Lane, Ackley Charles; Yu, Ziniu; Shu, Xiao; Leung, Priscilla TY (2013): Elevated CO2 alters larval proteome and its phosphorylation status in the commercial oyster, Crassostrea hongkongensis. Marine Biology, 160(8), 2189-2205 Dineshram, R Thiyagarajan, Vengatesen Lane, Ackley Charles Yu, Ziniu Shu, Xiao Leung, Priscilla TY 2013 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.831445 https://doi.pangaea.de/10.1594/PANGAEA.831445 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1007/s00227-013-2176-x https://cran.r-project.org/package=seacarb Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Animalia Coast and continental shelf Containers and aquaria 20-1000 L or < 1 m**2 Crassostrea hongkongensis Gene expression incl. proteomics Growth/Morphology Laboratory experiment Mollusca North Pacific Pelagos Single species Tropical Zooplankton Species Figure Treatment Replicates Incubation duration Shell length Growth rate Growth rate, standard deviation Protein name Protein spots, total Protein spots, total, standard deviation Spot intensity, relative Spot intensity, relative, standard deviation Temperature, water Salinity Alkalinity, total pH Carbon, inorganic, dissolved Partial pressure of carbon dioxide water at sea surface temperature wet air Carbon dioxide Bicarbonate ion Carbonate ion Calcite saturation state Aragonite saturation state Carbonate system computation flag Fugacity of carbon dioxide water at sea surface temperature wet air Potentiometric titration Potentiometric Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Supplementary Dataset dataset Dataset 2013 ftdatacite https://doi.org/10.1594/pangaea.831445 https://doi.org/10.1007/s00227-013-2176-x 2021-11-05T12:55:41Z Ocean acidification (OA) is beginning to have noticeable negative impact on calcification rate, shell structure and physiological energy budgeting of several marine organisms; these alter the growth of many economically important shellfish including oysters. Early life stages of oysters may be particularly vulnerable to OA-driven low pH conditions because their shell is made up of the highly soluble form of calcium carbonate (CaCO3) mineral, aragonite. Our long-term CO2 perturbation experiment showed that larval shell growth rate of the oyster species Crassostrea hongkongensis was significantly reduced at pH < 7.9 compared to the control (8.2). To gain new insights into the underlying mechanisms of low-pH-induced delays in larval growth, we have examined the effect of pH on the protein expression pattern, including protein phosphorylation status at the pediveliger larval stage. Using two-dimensional electrophoresis and mass spectrometry, we demonstrated that the larval proteome was significantly altered by the two low pH treatments (7.9 and 7.6) compared to the control pH (8.2). Generally, the number of expressed proteins and their phosphorylation level decreased with low pH. Proteins involved in larval energy metabolism and calcification appeared to be down-regulated in response to low pH, whereas cell motility and production of cytoskeletal proteins were increased. This study on larval growth coupled with proteome change is the first step toward the search for novel Protein Expression Signatures indicative of low pH, which may help in understanding the mechanisms involved in low pH tolerance. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-04-03. Dataset Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) Pacific |