Seawater carbonate chemistry, biomass and metabolic rates (leucine incorporation, CO2 fixation and respiration) of Rhodobacteraceae (strain MED165) and Flavobacteriaceae (strain MED217) in a laboratory experiment, supplement to: Teira, Eva; Fernández, Ana; Alvarez-Salgado, Xose Anton; García-Martín, Enma Elena; Serret, Pablo; Sobrino, Cristina (2012): Response of two marine bacterial isolates to high CO2 concentration. Marine Ecology Progress Series, 453, 27-36

• Main Authors: Teira, Eva, Fernández, A, Alvarez-Salgado, Xose Anton, García-Martín, Enma Elena, Serret, Pablo, Sobrino, Cristina
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2012
• Subjects: Bacteria; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Cytophaga sp.; Growth/Morphology; Heterotrophic prokaryotes; Laboratory experiment; Laboratory strains; Mediterranean Sea; Other metabolic rates; Pelagos; Respiration; Single species; Species; Strain; Treatment; pH; pH, standard error; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide water at sea surface temperature wet air, standard error; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard error; Alkalinity, total; Alkalinity, total, standard error; Temperature, water; Temperature, water, standard error; Salinity; Salinity, standard error; Fluorescence, dissolved organic matter; Fluorescence, dissolved organic matter, standard error; Fluorescence, particulate organic matter; Fluorescence, particulate organic matter, standard error; Abundance per volume; Abundance, standard error; Leucine incorporation rate; Leucine incorporation rate, standard error; Respiration rate, carbon dioxide; Respiration rate, carbon dioxide, standard error; Bacteria, growth efficiency; Bacteria, growth efficiency, standard error; Carbon fixation rate; Carbon fixation rate, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Potentiometric; Calculated; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Alvarez; Pablo; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.831372; https://doi.pangaea.de/10.1594/PANGAEA.831372

Experimental results related to the effects of ocean acidification on planktonic marine microbes are still rather inconsistent and occasionally contradictory. Moreover, laboratory or field experiments that address the effects of changes in CO2 concentrations on heterotrophic microbes are very scarce, despite the major role of these organisms in the marine carbon cycle. We tested the direct effect of an elevated CO2 concentration (1000 ppmv) on the biomass and metabolic rates (leucine incorporation, CO2 fixation and respiration) of 2 isolates belonging to 2 relevant marine bacterial families, Rhodobacteraceae (strain MED165) and Flavobacteriaceae (strain MED217). Our results demonstrate that, contrary to some expectations, high pCO2 did not negatively affect bacterial growth but increased growth efficiency in the case of MED217. The elevated partial pressure of CO2 (pCO2) caused, in both cases, higher rates of CO2 fixation in the dissolved fraction and, in the case of MED217, lower respiration rates. Both responses would tend to increase the pH of seawater acting as a negative feedback between elevated atmospheric CO2 concentrations and ocean acidification. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-04-01.