Larval and post-larval stages of pacific oyster (Crassostrea gigas) are resistant to elevated CO2, supplement to: Ko, W K Ginger; Chan, B S Vera; Dineshram, R; Choi, K S Dennis; Li, J Adela; Yu, Ziniu; Thiyagarajan, Vengatesen (2013): Larval and Post-Larval Stages of Pacific Oyster (Crassostrea gigas) Are Resistant to Elevated CO2. PLoS ONE, 8(5), e64147

• Main Authors: Ko, W K Ginger, Chan, B S Vera, Dineshram, R, Choi, K S Dennis, Li, J Adela, Yu, Ziniu, Thiyagarajan, Vengatesen
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2013
• Subjects: Animalia; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Crassostrea gigas; Development; Growth/Morphology; Laboratory experiment; Mollusca; North Pacific; Pelagos; Respiration; Single species; Temperate; Temperature; Zooplankton; Species; Figure; Treatment; Incubation duration; Stage; Percentage; Percentage, standard deviation; Size; Replicate; Individuals; Respiration rate, oxygen, per dry mass; Respiration rate, standard deviation; Filtering rate; Filtering rate, standard deviation; Biomass, ash free dry mass; Biomass, ash free dry mass, shell-free, standard deviation; Growth rate; Growth rate, standard deviation; pH; pH, standard deviation; Temperature, water; Temperature, standard deviation; Salinity; Salinity, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbonate ion; Calcite saturation state; Aragonite saturation state; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbon, inorganic, dissolved; Experiment; Potentiometric; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification; Pacific oyster
• Online Access: https://dx.doi.org/10.1594/pangaea.830882; https://doi.pangaea.de/10.1594/PANGAEA.830882

Rising anthropogenic carbon dioxide (CO2) dissolving into coastal waters is decreasing the pH and carbonate ion concentration, thereby lowering the saturation state of calcium carbonate (CaCO3) minerals through a process named ocean acidification (OA). The unprecedented threats posed by such low pH on calcifying larvae of several edible oyster species have not yet been fully explored. Effects of low pH (7.9, 7.6, 7.4) on the early growth phase of Portuguese oyster (Crassostrea angulata) veliger larvae was examined at ambient salinity (34 ppt) and the low-salinity (27 ppt) treatment. Additionally, the combined effect of pH (8.1, 7.6), salinity (24 and 34 ppt) and temperature (24 °C and 30 °C) was examined using factorial experimental design. Surprisingly, the early growth phase from hatching to 5-day-old veliger stage showed high tolerance to pH 7.9 and pH 7.6 at both 34 ppt and 27 ppt. Larval shell area was significantly smaller at pH 7.4 only in low-salinity. In the 3-factor experiment, shell area was affected by salinity and the interaction between salinity and temperature but not by other combinations. Larvae produced the largest shell at the elevated temperature in low-salinity, regardless of pH. Thus the growth of the Portuguese oyster larvae appears to be robust to near-future pH level (> 7.6) when combined with projected elevated temperature and low-salinity in the coastal aquaculture zones of South China Sea. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-03-21.