Consumers mediate the effects of experimental ocean acidification and warming on primary producers, supplement to: Alsterberg, Christian; Eklöf, Johan S; Gamfeldt, Lars; Havenhand, Jonathan N; Sundbäck, Kristina (2013): Consumers mediate the effects of experimental ocean acidification and warming on primary producers. Proceedings of the National Academy of Sciences, 110(21), 8603-8608

• Main Authors: Alsterberg, Christian, Eklöf, Johan S, Gamfeldt, Lars, Havenhand, Jonathan N, Sundbäck, Kristina
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2013
• Subjects: Benthos; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria <20 L; Coast and continental shelf; Entire community; Laboratory experiment; North Atlantic; Primary production/Photosynthesis; Soft-bottom community; Temperate; Temperature; Treatment; pH; Chlorophyll a, areal concentration; Primary production of carbon; Biomass, dry mass; Dry mass; Salinity; Salinity, standard deviation; Temperature, water; Temperature, water, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; pH, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Calcite saturation state; Calcite saturation state, standard deviation; Aragonite saturation state; Aragonite saturation state, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Experiment; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.830714; https://doi.pangaea.de/10.1594/PANGAEA.830714

It is well known that ocean acidification can have profound impacts on marine organisms. However, we know little about the direct and indirect effects of ocean acidification and also how these effects interact with other features of environmental change such as warming and declining consumer pressure. In this study, we tested whether the presence of consumers (invertebrate mesograzers) influenced the interactive effects of ocean acidification and warming on benthic microalgae in a seagrass community mesocosm experiment. Net effects of acidification and warming on benthic microalgal biomass and production, as assessed by analysis of variance, were relatively weak regardless of grazer presence. However, partitioning these net effects into direct and indirect effects using structural equation modeling revealed several strong relationships. In the absence of grazers, benthic microalgae were negatively and indirectly affected by sediment-associated microalgal grazers and macroalgal shading, but directly and positively affected by acidification and warming. Combining indirect and direct effects yielded no or weak net effects. In the presence of grazers, almost all direct and indirect climate effects were nonsignificant. Our analyses highlight that (i) indirect effects of climate change may be at least as strong as direct effects, (ii) grazers are crucial in mediating these effects, and (iii) effects of ocean acidification may be apparent only through indirect effects and in combination with other variables (e.g., warming). These findings highlight the importance of experimental designs and statistical analyses that allow us to separate and quantify the direct and indirect effects of multiple climate variables on natural communities. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-03-18.