Experiment: Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi ...

• Main Authors: Bach, Lennart Thomas, Mackinder, Luke C M
• Format: Dataset
• Language: English
• Published: PANGAEA 2013
• Subjects: Calcification/Dissolution; Chromista; Containers and aquaria 20-1000 L or < 1 m**2; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Species; Treatment; Irradiance; LightDark cycle; Temperature, water; Salinity; Carbon, inorganic, dissolved; Difference; pH; Bicarbonate ion; Carbonate ion; Carbon dioxide; Growth rate; Particulate inorganic carbon/particulate organic carbon ratio; Chlorophyll a; Particulate organic carbon production per cell; Particulate inorganic carbon production per cell; Carbon/Nitrogen ratio; Anion exchanger like 1; Anion exchanger like 1, standard error; alpha carbonic anhydrase 1; alpha carbonic anhydrase 1, standard error; alpha carbonic anhydrase 2; alpha carbonic anhydrase 2, standard error; beta carbonic anhydrase; beta carbonic anhydrase, standard error; gamma carbonic anhydrase, mean; gamma carbonic anhydrase, standard error; delta carbonic anhydrase; delta carbonic anhydrase, standard error; RubisCO; RubisCO, standard error; Voltage-gated H+ channel; Voltage-gated H+ channel, standard error; Vacuolar-type H+ pump; Vacuolar-type H+ pump, standard error; Plasma membran type H+ pump; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.830627; https://doi.pangaea.de/10.1594/PANGAEA.830627

Coccolithophores are important calcifying phytoplankton predicted to be impacted by changes in ocean carbonate chemistry caused by the absorption of anthropogenic CO2. However, it is difficult to disentangle the effects of the simultaneously changing carbonate system parameters (CO2, bicarbonate, carbonate and protons) on the physiological responses to elevated CO2. Here, we adopted a multifactorial approach at constant pH or CO2 whilst varying dissolved inorganic carbon (DIC) to determine physiological and transcriptional responses to individual carbonate system parameters. We show that Emiliania huxleyi is sensitive to low CO2 (growth and photosynthesis) and low bicarbonate (calcification) as well as low pH beyond a limited tolerance range, but is much less sensitive to elevated CO2 and bicarbonate. Multiple up-regulated genes at low DIC bear the hallmarks of a carbon-concentrating mechanism (CCM) that is responsive to CO2 and bicarbonate but not to pH. Emiliania huxleyi appears to have evolved mechanisms ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11. ...