Experiment: Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi ...
Coccolithophores are important calcifying phytoplankton predicted to be impacted by changes in ocean carbonate chemistry caused by the absorption of anthropogenic CO2. However, it is difficult to disentangle the effects of the simultaneously changing carbonate system parameters (CO2, bicarbonate, ca...
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Online Access: | https://dx.doi.org/10.1594/pangaea.830627 https://doi.pangaea.de/10.1594/PANGAEA.830627 |
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ftdatacite:10.1594/pangaea.830627 2024-04-28T08:35:03+00:00 Experiment: Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi ... Bach, Lennart Thomas Mackinder, Luke C M 2013 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.830627 https://doi.pangaea.de/10.1594/PANGAEA.830627 en eng PANGAEA https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1111/nph.12225 https://cran.r-project.org/package=seacarb Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 Calcification/Dissolution Chromista Containers and aquaria 20-1000 L or < 1 m**2 Emiliania huxleyi Growth/Morphology Haptophyta Laboratory experiment Laboratory strains Not applicable Pelagos Phytoplankton Primary production/Photosynthesis Single species Species Treatment Irradiance LightDark cycle Temperature, water Salinity Carbon, inorganic, dissolved Difference pH Bicarbonate ion Carbonate ion Carbon dioxide Growth rate Particulate inorganic carbon/particulate organic carbon ratio Chlorophyll a Particulate organic carbon production per cell Particulate inorganic carbon production per cell Carbon/Nitrogen ratio Anion exchanger like 1 Anion exchanger like 1, standard error alpha carbonic anhydrase 1 alpha carbonic anhydrase 1, standard error alpha carbonic anhydrase 2 alpha carbonic anhydrase 2, standard error beta carbonic anhydrase beta carbonic anhydrase, standard error gamma carbonic anhydrase, mean gamma carbonic anhydrase, standard error delta carbonic anhydrase delta carbonic anhydrase, standard error RubisCO RubisCO, standard error Voltage-gated H+ channel Voltage-gated H+ channel, standard error Vacuolar-type H+ pump Vacuolar-type H+ pump, standard error Plasma membran type H+ pump dataset Supplementary Dataset Dataset 2013 ftdatacite https://doi.org/10.1594/pangaea.83062710.1111/nph.12225 2024-04-02T10:25:46Z Coccolithophores are important calcifying phytoplankton predicted to be impacted by changes in ocean carbonate chemistry caused by the absorption of anthropogenic CO2. However, it is difficult to disentangle the effects of the simultaneously changing carbonate system parameters (CO2, bicarbonate, carbonate and protons) on the physiological responses to elevated CO2. Here, we adopted a multifactorial approach at constant pH or CO2 whilst varying dissolved inorganic carbon (DIC) to determine physiological and transcriptional responses to individual carbonate system parameters. We show that Emiliania huxleyi is sensitive to low CO2 (growth and photosynthesis) and low bicarbonate (calcification) as well as low pH beyond a limited tolerance range, but is much less sensitive to elevated CO2 and bicarbonate. Multiple up-regulated genes at low DIC bear the hallmarks of a carbon-concentrating mechanism (CCM) that is responsive to CO2 and bicarbonate but not to pH. Emiliania huxleyi appears to have evolved mechanisms ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11. ... Dataset Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Calcification/Dissolution Chromista Containers and aquaria 20-1000 L or < 1 m**2 Emiliania huxleyi Growth/Morphology Haptophyta Laboratory experiment Laboratory strains Not applicable Pelagos Phytoplankton Primary production/Photosynthesis Single species Species Treatment Irradiance LightDark cycle Temperature, water Salinity Carbon, inorganic, dissolved Difference pH Bicarbonate ion Carbonate ion Carbon dioxide Growth rate Particulate inorganic carbon/particulate organic carbon ratio Chlorophyll a Particulate organic carbon production per cell Particulate inorganic carbon production per cell Carbon/Nitrogen ratio Anion exchanger like 1 Anion exchanger like 1, standard error alpha carbonic anhydrase 1 alpha carbonic anhydrase 1, standard error alpha carbonic anhydrase 2 alpha carbonic anhydrase 2, standard error beta carbonic anhydrase beta carbonic anhydrase, standard error gamma carbonic anhydrase, mean gamma carbonic anhydrase, standard error delta carbonic anhydrase delta carbonic anhydrase, standard error RubisCO RubisCO, standard error Voltage-gated H+ channel Voltage-gated H+ channel, standard error Vacuolar-type H+ pump Vacuolar-type H+ pump, standard error Plasma membran type H+ pump |
spellingShingle |
Calcification/Dissolution Chromista Containers and aquaria 20-1000 L or < 1 m**2 Emiliania huxleyi Growth/Morphology Haptophyta Laboratory experiment Laboratory strains Not applicable Pelagos Phytoplankton Primary production/Photosynthesis Single species Species Treatment Irradiance LightDark cycle Temperature, water Salinity Carbon, inorganic, dissolved Difference pH Bicarbonate ion Carbonate ion Carbon dioxide Growth rate Particulate inorganic carbon/particulate organic carbon ratio Chlorophyll a Particulate organic carbon production per cell Particulate inorganic carbon production per cell Carbon/Nitrogen ratio Anion exchanger like 1 Anion exchanger like 1, standard error alpha carbonic anhydrase 1 alpha carbonic anhydrase 1, standard error alpha carbonic anhydrase 2 alpha carbonic anhydrase 2, standard error beta carbonic anhydrase beta carbonic anhydrase, standard error gamma carbonic anhydrase, mean gamma carbonic anhydrase, standard error delta carbonic anhydrase delta carbonic anhydrase, standard error RubisCO RubisCO, standard error Voltage-gated H+ channel Voltage-gated H+ channel, standard error Vacuolar-type H+ pump Vacuolar-type H+ pump, standard error Plasma membran type H+ pump Bach, Lennart Thomas Mackinder, Luke C M Experiment: Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi ... |
topic_facet |
Calcification/Dissolution Chromista Containers and aquaria 20-1000 L or < 1 m**2 Emiliania huxleyi Growth/Morphology Haptophyta Laboratory experiment Laboratory strains Not applicable Pelagos Phytoplankton Primary production/Photosynthesis Single species Species Treatment Irradiance LightDark cycle Temperature, water Salinity Carbon, inorganic, dissolved Difference pH Bicarbonate ion Carbonate ion Carbon dioxide Growth rate Particulate inorganic carbon/particulate organic carbon ratio Chlorophyll a Particulate organic carbon production per cell Particulate inorganic carbon production per cell Carbon/Nitrogen ratio Anion exchanger like 1 Anion exchanger like 1, standard error alpha carbonic anhydrase 1 alpha carbonic anhydrase 1, standard error alpha carbonic anhydrase 2 alpha carbonic anhydrase 2, standard error beta carbonic anhydrase beta carbonic anhydrase, standard error gamma carbonic anhydrase, mean gamma carbonic anhydrase, standard error delta carbonic anhydrase delta carbonic anhydrase, standard error RubisCO RubisCO, standard error Voltage-gated H+ channel Voltage-gated H+ channel, standard error Vacuolar-type H+ pump Vacuolar-type H+ pump, standard error Plasma membran type H+ pump |
description |
Coccolithophores are important calcifying phytoplankton predicted to be impacted by changes in ocean carbonate chemistry caused by the absorption of anthropogenic CO2. However, it is difficult to disentangle the effects of the simultaneously changing carbonate system parameters (CO2, bicarbonate, carbonate and protons) on the physiological responses to elevated CO2. Here, we adopted a multifactorial approach at constant pH or CO2 whilst varying dissolved inorganic carbon (DIC) to determine physiological and transcriptional responses to individual carbonate system parameters. We show that Emiliania huxleyi is sensitive to low CO2 (growth and photosynthesis) and low bicarbonate (calcification) as well as low pH beyond a limited tolerance range, but is much less sensitive to elevated CO2 and bicarbonate. Multiple up-regulated genes at low DIC bear the hallmarks of a carbon-concentrating mechanism (CCM) that is responsive to CO2 and bicarbonate but not to pH. Emiliania huxleyi appears to have evolved mechanisms ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11. ... |
format |
Dataset |
author |
Bach, Lennart Thomas Mackinder, Luke C M |
author_facet |
Bach, Lennart Thomas Mackinder, Luke C M |
author_sort |
Bach, Lennart Thomas |
title |
Experiment: Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi ... |
title_short |
Experiment: Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi ... |
title_full |
Experiment: Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi ... |
title_fullStr |
Experiment: Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi ... |
title_full_unstemmed |
Experiment: Dissecting the impact of CO2 and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi ... |
title_sort |
experiment: dissecting the impact of co2 and ph on the mechanisms of photosynthesis and calcification in the coccolithophore emiliania huxleyi ... |
publisher |
PANGAEA |
publishDate |
2013 |
url |
https://dx.doi.org/10.1594/pangaea.830627 https://doi.pangaea.de/10.1594/PANGAEA.830627 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1111/nph.12225 https://cran.r-project.org/package=seacarb |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_doi |
https://doi.org/10.1594/pangaea.83062710.1111/nph.12225 |
_version_ |
1797591521313161216 |