Experiment: Organic matter exudation by Emiliania huxleyi under simulated future ocean conditions ...

• Main Authors: Borchard, Corinna, Engel, Anja
• Format: Dataset
• Language: English
• Published: PANGAEA 2012
• Subjects: Bottles or small containers/Aquaria <20 L; Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Other metabolic rates; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Temperature; Species; Replicate; Treatment; Growth rate; Exudation as determined by 14C DOC production; Primary production of POC as determined by 14C POC production; Primary production of carbon per day; Extracellular release; Carbohydrates, dissolved hydrolyzable; Carbohydrates, total combined; Carbohydrates, particulate hydrolyzable; Fucose; Rhamnose; Arabinose/Galactosamine; Glucosamine; Galactose; Glucose; Mannose/Xylose; Galacturonic acid; Glucuronic acid; Transparent exopolymer particles; Carbon, organic, dissolved; Transparent exopolymer particles/Carbon, organic, particulate ratio; Salinity; Temperature, water; Alkalinity, total; pH; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbon dioxide; Bicarbonate ion; Carbonate ion; Calcite saturation state; Revelle factor; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.829883; https://doi.pangaea.de/10.1594/PANGAEA.829883

Emiliania huxleyi (strain B 92/11) was exposed to different nutrient supply, CO2 and temperature conditions in phosphorus controlled chemostats to investigate effects on organic carbon exudation and partitioning between the pools of particulate organic carbon (POC) and dissolved organic carbon (DOC). 14C incubation measurements for primary production (PP) and extracellular release (ER) were performed. Chemical analysis included the amount and composition of high molecular weight (>1 kDa) dissolved combined carbohydrates (HMW-dCCHO), particulate combined carbohydrates (pCCHO) and the carbon content of transparent exopolymer particles (TEP-C). Applied CO2 and temperature conditions were 300, 550 and 900 µatm pCO2 at 14 °C, and additionally 900 µatm pCO2 at 18 °C simulating a greenhouse ocean scenario.Enhanced nutrient stress by reducing the dilution rate (D) from D = 0.3 /d to D = 0.1 /d (D = µ) induced the strongest response in E. huxleyi. At µ = 0.3 /d, PP was significantly higher at elevated CO2 and ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11. ...