Response of Nodularia spumigena to pCO2 ...

• Main Authors: Endres, Sonja, Unger, Juliane, Wannicke, Nicola, Nausch, Monika, Voss, Maren, Engel, Anja
• Format: Dataset
• Language: English
• Published: PANGAEA 2013
• Subjects: Nodularia spumigena; Not applicable; Species; DATE/TIME; Incubation duration; Treatment; Phosphate; Phosphate, standard deviation; Nitrogen, inorganic, dissolved; Nitrogen, inorganic, dissolved, standard deviation; Chlorophyll a; Chlorophyll a, standard deviation; Bacteria, abundance; Bacteria, abundance, standard deviation; Carbon, organic, dissolved; Carbon, organic, dissolved, standard deviation; Nitrogen, organic, dissolved; Standard deviation; Phosphate, organic, dissolved; Mucinous substances; Coomassie stainable particles; Coomassie stainable particles, abundance; Coomassie stainable particles, equivalent spherical diameter; alpha-glucosidase activity; beta-glucosidase activity; Leucine aminopeptidase activity; Alkaline phosphatase activity; Alkaline phosphatase, Km value; Temperature, water; Salinity; pH; pH, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Spectrophotometer Hitachi U-2000; Fluorometry; Flow cytometry; TOC analyzer Shimadzu; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.829084; https://doi.pangaea.de/10.1594/PANGAEA.829084

The filamentous and diazotrophic cyanobacterium Nodularia spumigena plays a major role in the productivity of the Baltic Sea as it forms extensive blooms regularly. Under phosphorus limiting conditions Nodularia spumigena has a high enzyme affinity for dissolved organic phosphorus (DOP) by production and release of alkaline phosphatase. Additionally, it is able to degrade proteinaceous compounds by expressing the extracellular enzyme leucine aminopeptidase. As atmospheric CO2 concentrations are increasing, we expect marine phytoplankton to experience changes in several environmental parameters including pH, temperature, and nutrient availability. The aim of this study was to investigate the combined effect of CO2-induced changes in seawater carbonate chemistry and of phosphate deficiency on the exudation of organic matter, and its subsequent recycling by extracellular enzymes in a Nodularia spumigena culture. Batch cultures of Nodularia spumigena were grown for 15 days aerated with three different pCO2 ... : Values are means and standard deviations of three replicates (except one replicate bottle of the 180 ppm treatment at day 9).In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-02-11. ...