Physiological responses of the marine diatom Thalassiosira pseudonana to increased pCO2 and seawater acidity, supplement to: Yang, Gui yuan; Gao, Kunshan (2012): Physiological responses of the marine diatom Thalassiosira pseudonana to increased pCO2 and seawater acidity. Marine Environmental Research, 79, 142-151

• Main Authors: Yang, Gui yuan, Gao, Kunshan
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2012
• Subjects: Laboratory experiment; Laboratory strains; Light; North Pacific; Ochrophyta; Phytoplankton; Respiration; Thalassiosira pseudonana; Treatment; Identification; Species; Carbon, inorganic, dissolved; Irradiance; Photosynthetic carbon fixation rate per cell; Photosynthetic carbon fixation rate, standard deviation; Maximal electron transport rate, relative; Electron transport rate, relative, standard deviation; Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value; Carbon, inorganic, dissolved, reciprocal of photosynthetic affinity value, standard deviation; Carbon dioxide, reciprocal of photosynthetic affinity value; Carbon dioxide, reciprocal of photosynthetic affinity value, standard deviation; Photosynthetic carbon fixation per cell, maximum velocity; Photosynthetic carbon fixation, maximum velocity, standard deviation; Electron transport rate, relative, maximum velocity; Electron transport rate, relative, maximum velocity, standard deviation; Growth rate; Growth rate, standard deviation; Chlorophyll a per cell; Chlorophyll a, standard deviation; Chlorophyll c per cell; Chlorophyll c, standard deviation; Gross carbon fixation rate per cell; Gross carbon fixation rate, standard deviation; Respiration rate, oxygen, per cell; Respiration rate, oxygen, standard deviation; Photosynthetic efficiency; Photosynthetic efficiency, standard deviation; Light saturation point; Light saturation point, standard deviation; Time in seconds; Effective quantum yield; Effective quantum yield, standard deviation; Effective absorbance cross-section of photosystem II; Effective absorbance cross-section of photosystem II, standard deviation; Non photochemical quenching; Non photochemical quenching, standard deviation; Temperature, water; Salinity; pH; pH, standard deviation; Carbon, inorganic, dissolved, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Carbon dioxide; Carbon dioxide, standard deviation; Alkalinity, total; Alkalinity, total, standard deviation; Carbonate system computation flag; Partial pressure of carbon dioxide water at sea surface temperature wet air; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.826461; https://doi.pangaea.de/10.1594/PANGAEA.826461

We studied the effects of elevated CO2 concentration and seawater acidity on inorganic carbon acquisition, photoinhibition and photoprotection as well as growth and respiration in the marine diatom Thalassiosira pseudonana. After having grown under the elevated CO2 level (1000 µatm, pH 7.83) at sub-saturating photosynthetically active radiation (PAR, 75 µmol photons/m**2/s) for 20 generations, photosynthesis and dark respiration of the alga increased by 25% (14.69 ± 2.55 fmol C/cell/h) and by 35% (4.42 ± 0.98 fmol O2/cell/h), respectively, compared to that grown under the ambient CO2 level (390 µatm, pH 8.16), leading to insignificant effects on growth (1.09 ± 0.08 (1/d))v 1.04 ± 0.07 (1/d)). The photosynthetic affinity for CO2 was lowered in the high-CO2 grown cells, reflecting a down-regulation of the CO2 concentrating mechanism (CCM). When exposed to an excessively high level of PAR, photochemical and non-photochemical quenching responded similarly in the low- and high-CO2 grown cells, reflecting that photoinhibition was not influenced by the enriched level of CO2. In T. pseudonana, it appeared that the energy saved due to the down-regulated CCM did not contribute to any additional light stress as previously found in another diatom Phaeodactylum tricornutum, indicating differential physiological responses to ocean acidification between these two diatom species. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-01-20.