Seawater carbonate chemistry and photosystem II photoinactivation of the coastal diatom Thalassiosira pseudonana in a laboratory experiment, supplement to: Li, Gang; Campbell, Douglas A (2013): Rising CO2 Interacts with Growth Light and Growth Rate to Alter Photosystem II Photoinactivation of the Coastal Diatom Thalassiosira pseudonana. PLoS ONE, 8(1), e55562

• Main Authors: Li, Gang, Campbell, Douglas A
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2013
• Subjects: Bottles or small containers/Aquaria <20 L; Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira pseudonana; Identification; Species; Treatment; Replicates; Date; Irradiance; Time in minutes; Cell density; Cell biovolume; Growth rate; Effective absorbance cross-section of photosystem II; Photochemical quenching; Protein per cell; Photosynthetic protein PsbA; Photosynthetic protein PsbD; Photosynthetic protein Rubisco; CF1 subunit of ATP synthase protein; Cytochrome c1; Chlorophyll a per cell; Carbon, organic, particulate, per cell; Nitrogen, organic, particulate, per cell; Carbon/Nitrogen ratio; Temperature, water; Salinity; Carbon, inorganic, dissolved; pH; Partial pressure of carbon dioxide water at sea surface temperature wet air; Alkalinity, total; Bicarbonate ion; Carbonate ion; Carbon dioxide; Phosphate; Silicate; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Photospectrometer, methylene blue; Element analyser CNS, Elementar Vario EL; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Psi; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.824663; https://doi.pangaea.de/10.1594/PANGAEA.824663

We studied the interactive effects of pCO2 and growth light on the coastal marine diatom Thalassiosira pseudonana CCMP 1335 growing under ambient and expected end-of-the-century pCO2 (750 ppmv), and a range of growth light from 30 to 380 µmol photons/m**2/s. Elevated pCO2 significantly stimulated the growth of T. pseudonana under sub-saturating growth light, but not under saturating to super-saturating growth light. Under ambient pCO2 susceptibility to photoinactivation of photosystem II (sigma i) increased with increasing growth rate, but cells growing under elevated pCO2 showed no dependence between growth rate and sigma i, so under high growth light cells under elevated pCO2 were less susceptible to photoinactivation of photosystem II, and thus incurred a lower running cost to maintain photosystem II function. Growth light altered the contents of RbcL (RUBISCO) and PsaC (PSI) protein subunits, and the ratios among the subunits, but there were only limited effects on these and other protein pools between cells grown under ambient and elevated pCO2. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-12-16.