Seawater carbonate chemistry and growth, carbon acquisition, and species interaction of Antarctic phytoplankton species in a laboratory experiment ...

• Main Authors: Trimborn, Scarlett, Brenneis, Tina, Sweet, Elizabeth, Rost, Björn
• Format: Dataset
• Language: English
• Published: PANGAEA 2013
• Subjects: Antarctic; Bottles or small containers/Aquaria <20 L; Chaetoceros debilis; Chromista; Fragilariopsis kerguelensis; Growth/Morphology; Laboratory experiment; Laboratory strains; Ochrophyta; Pelagos; Phaeocystis antarctica; Phytoplankton; Primary production/Photosynthesis; Pseudo-nitzschia subcurvata; Single species; Species interaction; Identification; Species; Description; Treatment; Growth rate; Growth rate, standard deviation; Bicarbonate uptake/net fixation ratio; Bicarbonate uptake/net fixation ratio, standard deviation; Extracellular carbonic anhydrase activity; Extracellular carbonic anhydrase activity, standard deviation; Chaetoceros debilis/Pseudo-nitzschia subcurvata ratio; Chaetoceros debilis/Pseudo-nitzschia subcurvata ratio, standard deviation; Gross carbon dioxide uptake/net fixation ratio; Gross carbon dioxide uptake/net fixation ratio, standard deviation; Cell surface area/cell volume ratio; Cell surface area/cell volume, standard deviation; Carbon dioxide, reciprocal of photosynthetic affinity value; Carbon dioxide, reciprocal of photosynthetic affinity value, standard deviation; Photosynthesis carbon dioxide uptake rate, maximum velocity; Photosynthesis carbon dioxide uptake, maximum velocity, standard deviation; Gross carbon dioxide uptake, half saturation concentration; Gross carbon dioxide uptake, half saturation concentration, standard deviation; Gross carbon dioxide uptake rate, per chlorophyll a, maximum velocity; Gross carbon dioxide uptake per chlorophyll a, maximum velocity, standard deviation; Net carbon dioxide uptake, half saturation concentration; Net carbon dioxide uptake, half saturation concentration, standard deviation; Net carbon dioxide uptake rate, per chlorophyll a, maximum velocity; Net carbon dioxide uptake per chlorophyll a, maximum velocity, standard deviation; Bicarbonate ion, reciprocal of photosynthetic affinity value; Bicarbonate ion, reciprocal of photosynthetic affinity value, standard deviation; Bicarbonate uptake rate, per chlorophyll a, maximum velocity; Bicarbonate uptake per chlorophyll a, maximum velocity, standard deviation; Temperature, water; Salinity; Southern Ocean; Antarc*; Antarctica; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.824406; https://doi.pangaea.de/10.1594/PANGAEA.824406

Despite the fact that ocean acidification is considered to be especially pronounced in the Southern Ocean, little is known about CO2-dependent physiological processes and the interactions of Antarctic phytoplankton key species. We therefore studied the effects of CO2 partial pressure (PCO2) (16.2, 39.5, and 101.3 Pa) on growth and photosynthetic carbon acquisition in the bloom-forming species Chaetoceros debilis, Pseudo-nitzschia subcurvata, Fragilariopsis kerguelensis, and Phaeocystis antarctica. Using membrane-inlet mass spectrometry, photosynthetic O2 evolution and inorganic carbon (Ci) fluxes were determined as a function of CO2 concentration. Only the growth of C. debilis was enhanced under high PCO2. Analysis of the carbon concentrating mechanism (CCM) revealed the operation of very efficient CCMs (i.e., high Ci affinities) in all species, but there were species-specific differences in CO2-dependent regulation of individual CCM components (i.e., CO2 and uptake kinetics, carbonic anhydrase activities). ... : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-12-10. ...