Seawater carbonate chemistry and survival, impairment of three phytoplankton species in a laboratory experiment, supplement to: Kim, Keunyong; Kim, Kwang Young; Kim, Ju Hyoung; Kang, Eun Ju; Jeong, Hae Jin; Lee, Kitack (2013): Synergistic effects of elevated carbon dioxide and sodium hypochlorite on survival and impairment of three phytoplankton species. ALGAE, 28(2), 173-183

• Main Authors: Kim, Keunyong, Kim, Kwang Young, Kim, Ju Hyoung, Kang, Eun Ju, Jeong, Hae Jin, Lee, Kitack
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2013
• Subjects: Akashiwo sanguinea; Bottles or small containers/Aquaria <20 L; Chromista; Heterosigma akashiwo; Laboratory experiment; Laboratory strains; Mortality/Survival; Myzozoa; North Pacific; Ochrophyta; Organic toxins; Pelagos; Phytoplankton; Primary production/Photosynthesis; Single species; Thalassiosira eccentrica; Identification; Species; Incubation duration; Treatment; Replicate; Total residual chlorine; Sodium hypochlorite; Abundance per volume; Chlorophyll a; Effective quantum yield; Lethal concentration 50; Lethal concentration 50, standard deviation; Temperature, water; Salinity; pH; Alkalinity, total; Alkalinity, total, standard deviation; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, standard deviation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Partial pressure of carbon dioxide, standard deviation; Bicarbonate ion; Bicarbonate ion, standard deviation; Carbonate ion; Carbonate ion, standard deviation; Hypochlorous acid; Hypochlorite; Carbonate system computation flag; Carbon dioxide; Fugacity of carbon dioxide water at sea surface temperature wet air; Aragonite saturation state; Calcite saturation state; Iodometric chemical method; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Pacific; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.824065; https://doi.pangaea.de/10.1594/PANGAEA.824065

Sodium hypochlorite (NaOCl) is widely used to disinfect seawater in power plant cooling systems in order to reduce biofouling, and in ballast water treatment systems to prevent transport of exotic marine species. While the toxicity of NaOCl is expected to increase by ongoing ocean acidification, and many experimental studies have shown how algal calcification, photosynthesis and growth respond to ocean acidification, no studies have investigated the relationship between NaOCl toxicity and increased CO2. Therefore, we investigated whether the impacts of NaOCl on survival, chlorophyll a (Chl-a), and effective quantum yield in three marine phytoplankton belonging to different taxonomic classes are increased under high CO2 levels. Our results show that all biological parameters of the three species decreased under increasing NaOCl concentration, but increasing CO2 concentration alone (from 450 to 715 µatm) had no effect on any of these parameters in the organisms. However, due to the synergistic effects between NaOCl and CO2, the survival and Chl-a content in two of the species, Thalassiosira eccentrica and Heterosigma akashiwo, were significantly reduced under high CO2 when NaOCl was also elevated. The results show that combined exposure to high CO2 and NaOCl results in increasing toxicity of NaOCl in some marine phytoplankton. Consequently, greater caution with use of NaOCl will be required, as its use is widespread in coastal waters. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-12-10.