Seawater carbonate chemistry and physiological and mechanical properties of the starfish Asterias rubens in a laboratory experiment, supplement to: Collard, Marie; Catarino, Ana Isabel; Bonnet, Stéphanie; Flammang, Patrick; Dubois, Philippe (2013): Effects of CO2-induced ocean acidification on physiological and mechanical properties of the starfish Asterias rubens. Journal of Experimental Marine Biology and Ecology, 446, 355-362

• Main Authors: Collard, Marie, Catarino, Ana Isabel, Bonnet, Stéphanie, Flammang, Patrick, Dubois, Philippe
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2013
• Subjects: Acid-base regulation; Animalia; Asterias rubens; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria 20-1000 L or < 1 m**2; Echinodermata; Gene expression incl. proteomics; Growth/Morphology; Laboratory experiment; North Atlantic; Other studied parameter or process; Respiration; Single species; Temperate; Species; Comment; Identification; Treatment; Sample code/label; Replicate; Incubation duration; Dry mass; Coelomic fluid, pH; Wet mass; Respiration rate, oxygen; Sample, optional label/labor no; Force; Extensibility; Strength; Toughness; Stiffness; Coelomic fluid, magnesium ion; Coelomic fluid, magnesium ion, standard deviation; Magnesium ion; Magnesium, standard deviation; Coelomic fluid, calcium ion; Coelomic fluid, calcium ion, standard deviation; Calcium ion; Calcium, standard deviation; Position; RNA/DNA ratio; RNA/DNA ratio, standard deviation; Salinity; Temperature, water; Alkalinity, total; pH; Carbon, inorganic, dissolved; Partial pressure of carbon dioxide water at sea surface temperature wet air; Carbon dioxide; Bicarbonate ion; Carbonate ion; Calcite saturation state; Aragonite saturation state; Carbonate system computation flag; Fugacity of carbon dioxide water at sea surface temperature wet air; ICP-AES, Inductively coupled plasma - atomic emission spectroscopy; Potentiometric titration; Potentiometric; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Ocean Acidification International Coordination Centre OA-ICC; Collard; DuBois; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.823583; https://doi.pangaea.de/10.1594/PANGAEA.823583

The increase in atmospheric CO2 due to anthropogenic activity results in an acidification of the surface waters of the oceans. Its impact will depend on the considered organisms and ecosystems. The intertidal may harbor organisms pre-adapted to the upcoming changes as they face tidal pH and temperature fluctuations. However, these environments will be more affected as shallow waters will face the highest decrease in seawater pH. In this context, the effects of reduced environmental pH on the physiology and tube feet mechanical properties of the intertidal starfish Asterias rubens, a top predator, were investigated during 15 and 27 days. A. rubens showed a respiratory acidosis with its coelomic fluid pH always lower than that of seawater. This acidosis was most pronounced at pH 7.4. Notwithstanding, the starfish showed no significant variations in RNA/DNA ratio of different tissues and in tube feet strength. However, respiration rates were significantly lower for individuals maintained at reduced seawater pH. Within the ocean acidification context, the present results suggest that A. rubens withstands the effects of reduced seawater pH, at least for medium term exposures. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-29.