Seawater carbonate chemistry and toxicity of Pseudo-nitzschia fraudulenta in a laboratory experiment, supplement to: Tatters, Avery O; Fu, Feixue; Hutchins, David A (2012): High CO2 and Silicate Limitation Synergistically Increase the Toxicity of Pseudo-nitzschia fraudulenta. PLoS ONE, 7(2), e32116
Anthropogenic CO2 is progressively acidifying the ocean, but the responses of harmful algal bloom species that produce toxins that can bioaccumulate remain virtually unknown. The neurotoxin domoic acid is produced by the globally-distributed diatom genus Pseudo-nitzschia. This toxin is responsible f...
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Format: | Dataset |
Language: | English |
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PANGAEA - Data Publisher for Earth & Environmental Science
2012
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Subjects: | |
Online Access: | https://dx.doi.org/10.1594/pangaea.823383 https://doi.pangaea.de/10.1594/PANGAEA.823383 |
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ftdatacite:10.1594/pangaea.823383 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
DataCite Metadata Store (German National Library of Science and Technology) |
op_collection_id |
ftdatacite |
language |
English |
topic |
Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Chromista Coast and continental shelf Growth/Morphology Immunology/Self-protection Laboratory experiment Macro-nutrients North Pacific Ochrophyta Pelagos Phytoplankton Pseudo-nitzschia fraudulenta Single species Temperate Species Treatment Replicate Biogenic silica Carbon, organic, particulate Nitrogen, organic, particulate Phosphorus, organic, particulate Domoic acid per cell Domoic acid production per cell Growth rate Cell density Carbon, inorganic, dissolved pH Temperature, water Salinity Carbonate system computation flag Carbon dioxide Partial pressure of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Experiment High Performance Liquid Chromatography HPLC Coulometric titration Potentiometric Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
spellingShingle |
Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Chromista Coast and continental shelf Growth/Morphology Immunology/Self-protection Laboratory experiment Macro-nutrients North Pacific Ochrophyta Pelagos Phytoplankton Pseudo-nitzschia fraudulenta Single species Temperate Species Treatment Replicate Biogenic silica Carbon, organic, particulate Nitrogen, organic, particulate Phosphorus, organic, particulate Domoic acid per cell Domoic acid production per cell Growth rate Cell density Carbon, inorganic, dissolved pH Temperature, water Salinity Carbonate system computation flag Carbon dioxide Partial pressure of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Experiment High Performance Liquid Chromatography HPLC Coulometric titration Potentiometric Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Tatters, Avery O Fu, Feixue Hutchins, David A Seawater carbonate chemistry and toxicity of Pseudo-nitzschia fraudulenta in a laboratory experiment, supplement to: Tatters, Avery O; Fu, Feixue; Hutchins, David A (2012): High CO2 and Silicate Limitation Synergistically Increase the Toxicity of Pseudo-nitzschia fraudulenta. PLoS ONE, 7(2), e32116 |
topic_facet |
Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Chromista Coast and continental shelf Growth/Morphology Immunology/Self-protection Laboratory experiment Macro-nutrients North Pacific Ochrophyta Pelagos Phytoplankton Pseudo-nitzschia fraudulenta Single species Temperate Species Treatment Replicate Biogenic silica Carbon, organic, particulate Nitrogen, organic, particulate Phosphorus, organic, particulate Domoic acid per cell Domoic acid production per cell Growth rate Cell density Carbon, inorganic, dissolved pH Temperature, water Salinity Carbonate system computation flag Carbon dioxide Partial pressure of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Experiment High Performance Liquid Chromatography HPLC Coulometric titration Potentiometric Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC |
description |
Anthropogenic CO2 is progressively acidifying the ocean, but the responses of harmful algal bloom species that produce toxins that can bioaccumulate remain virtually unknown. The neurotoxin domoic acid is produced by the globally-distributed diatom genus Pseudo-nitzschia. This toxin is responsible for amnesic shellfish poisoning, which can result in illness or death in humans and regularly causes mass mortalities of marine mammals and birds. Domoic acid production by Pseudo-nitzschia cells is known to be regulated by nutrient availability, but potential interactions with increasing seawater CO2 concentrations are poorly understood. Here we present experiments measuring domoic acid production by acclimatized cultures of Pseudo-nitzschia fraudulenta that demonstrate a strong synergism between projected future CO2 levels (765 ppm) and silicate-limited growth, which greatly increases cellular toxicity relative to growth under modern atmospheric (360 ppm) or pre-industrial (200 ppm) CO2 conditions. Cellular Si:C ratios decrease with increasing CO2, in a trend opposite to that seen for domoic acid production. The coastal California upwelling system where this species was isolated currently exhibits rapidly increasing levels of anthropogenic acidification, as well as widespread episodic silicate limitation of diatom growth. Our results suggest that the current ecosystem and human health impacts of toxic Pseudo-nitzschia blooms could be greatly exacerbated by future ocean acidification and 'carbon fertilization' of the coastal ocean. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-28. |
format |
Dataset |
author |
Tatters, Avery O Fu, Feixue Hutchins, David A |
author_facet |
Tatters, Avery O Fu, Feixue Hutchins, David A |
author_sort |
Tatters, Avery O |
title |
Seawater carbonate chemistry and toxicity of Pseudo-nitzschia fraudulenta in a laboratory experiment, supplement to: Tatters, Avery O; Fu, Feixue; Hutchins, David A (2012): High CO2 and Silicate Limitation Synergistically Increase the Toxicity of Pseudo-nitzschia fraudulenta. PLoS ONE, 7(2), e32116 |
title_short |
Seawater carbonate chemistry and toxicity of Pseudo-nitzschia fraudulenta in a laboratory experiment, supplement to: Tatters, Avery O; Fu, Feixue; Hutchins, David A (2012): High CO2 and Silicate Limitation Synergistically Increase the Toxicity of Pseudo-nitzschia fraudulenta. PLoS ONE, 7(2), e32116 |
title_full |
Seawater carbonate chemistry and toxicity of Pseudo-nitzschia fraudulenta in a laboratory experiment, supplement to: Tatters, Avery O; Fu, Feixue; Hutchins, David A (2012): High CO2 and Silicate Limitation Synergistically Increase the Toxicity of Pseudo-nitzschia fraudulenta. PLoS ONE, 7(2), e32116 |
title_fullStr |
Seawater carbonate chemistry and toxicity of Pseudo-nitzschia fraudulenta in a laboratory experiment, supplement to: Tatters, Avery O; Fu, Feixue; Hutchins, David A (2012): High CO2 and Silicate Limitation Synergistically Increase the Toxicity of Pseudo-nitzschia fraudulenta. PLoS ONE, 7(2), e32116 |
title_full_unstemmed |
Seawater carbonate chemistry and toxicity of Pseudo-nitzschia fraudulenta in a laboratory experiment, supplement to: Tatters, Avery O; Fu, Feixue; Hutchins, David A (2012): High CO2 and Silicate Limitation Synergistically Increase the Toxicity of Pseudo-nitzschia fraudulenta. PLoS ONE, 7(2), e32116 |
title_sort |
seawater carbonate chemistry and toxicity of pseudo-nitzschia fraudulenta in a laboratory experiment, supplement to: tatters, avery o; fu, feixue; hutchins, david a (2012): high co2 and silicate limitation synergistically increase the toxicity of pseudo-nitzschia fraudulenta. plos one, 7(2), e32116 |
publisher |
PANGAEA - Data Publisher for Earth & Environmental Science |
publishDate |
2012 |
url |
https://dx.doi.org/10.1594/pangaea.823383 https://doi.pangaea.de/10.1594/PANGAEA.823383 |
long_lat |
ENVELOPE(-65.433,-65.433,-66.883,-66.883) |
geographic |
Avery Pacific |
geographic_facet |
Avery Pacific |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1371/journal.pone.0032116.g004 https://cran.r-project.org/package=seacarb |
op_rights |
Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1594/pangaea.823383 https://doi.org/10.1371/journal.pone.0032116.g004 |
_version_ |
1766157800426176512 |
spelling |
ftdatacite:10.1594/pangaea.823383 2023-05-15T17:50:52+02:00 Seawater carbonate chemistry and toxicity of Pseudo-nitzschia fraudulenta in a laboratory experiment, supplement to: Tatters, Avery O; Fu, Feixue; Hutchins, David A (2012): High CO2 and Silicate Limitation Synergistically Increase the Toxicity of Pseudo-nitzschia fraudulenta. PLoS ONE, 7(2), e32116 Tatters, Avery O Fu, Feixue Hutchins, David A 2012 text/tab-separated-values https://dx.doi.org/10.1594/pangaea.823383 https://doi.pangaea.de/10.1594/PANGAEA.823383 en eng PANGAEA - Data Publisher for Earth & Environmental Science https://cran.r-project.org/package=seacarb https://dx.doi.org/10.1371/journal.pone.0032116.g004 https://cran.r-project.org/package=seacarb Creative Commons Attribution 3.0 Unported https://creativecommons.org/licenses/by/3.0/legalcode cc-by-3.0 CC-BY Biomass/Abundance/Elemental composition Bottles or small containers/Aquaria <20 L Chromista Coast and continental shelf Growth/Morphology Immunology/Self-protection Laboratory experiment Macro-nutrients North Pacific Ochrophyta Pelagos Phytoplankton Pseudo-nitzschia fraudulenta Single species Temperate Species Treatment Replicate Biogenic silica Carbon, organic, particulate Nitrogen, organic, particulate Phosphorus, organic, particulate Domoic acid per cell Domoic acid production per cell Growth rate Cell density Carbon, inorganic, dissolved pH Temperature, water Salinity Carbonate system computation flag Carbon dioxide Partial pressure of carbon dioxide water at sea surface temperature wet air Fugacity of carbon dioxide water at sea surface temperature wet air Bicarbonate ion Carbonate ion Alkalinity, total Aragonite saturation state Calcite saturation state Experiment High Performance Liquid Chromatography HPLC Coulometric titration Potentiometric Calculated using seacarb after Nisumaa et al. 2010 Ocean Acidification International Coordination Centre OA-ICC Supplementary Dataset dataset Dataset 2012 ftdatacite https://doi.org/10.1594/pangaea.823383 https://doi.org/10.1371/journal.pone.0032116.g004 2022-02-08T16:27:35Z Anthropogenic CO2 is progressively acidifying the ocean, but the responses of harmful algal bloom species that produce toxins that can bioaccumulate remain virtually unknown. The neurotoxin domoic acid is produced by the globally-distributed diatom genus Pseudo-nitzschia. This toxin is responsible for amnesic shellfish poisoning, which can result in illness or death in humans and regularly causes mass mortalities of marine mammals and birds. Domoic acid production by Pseudo-nitzschia cells is known to be regulated by nutrient availability, but potential interactions with increasing seawater CO2 concentrations are poorly understood. Here we present experiments measuring domoic acid production by acclimatized cultures of Pseudo-nitzschia fraudulenta that demonstrate a strong synergism between projected future CO2 levels (765 ppm) and silicate-limited growth, which greatly increases cellular toxicity relative to growth under modern atmospheric (360 ppm) or pre-industrial (200 ppm) CO2 conditions. Cellular Si:C ratios decrease with increasing CO2, in a trend opposite to that seen for domoic acid production. The coastal California upwelling system where this species was isolated currently exhibits rapidly increasing levels of anthropogenic acidification, as well as widespread episodic silicate limitation of diatom growth. Our results suggest that the current ecosystem and human health impacts of toxic Pseudo-nitzschia blooms could be greatly exacerbated by future ocean acidification and 'carbon fertilization' of the coastal ocean. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-28. Dataset Ocean acidification DataCite Metadata Store (German National Library of Science and Technology) Avery ENVELOPE(-65.433,-65.433,-66.883,-66.883) Pacific |