Seawater carbonate chemistry and growth rate of Emiliania huxleyi in lab experiment, supplement to: Lohbeck, Kai T; Riebesell, Ulf; Collins, Sinéad; Reusch, Thorsten B H (2013): Functional genetic divergence in high CO2 adapted Emiliania Huxleyi populations. Evolution, 67(7), 1892-1900

• Main Authors: Lohbeck, Kai T, Riebesell, Ulf, Collins, Sinéad, Reusch, Thorsten B H
• Format: Dataset
• Language: English
• Published: PANGAEA - Data Publisher for Earth & Environmental Science 2013
• Subjects: Bottles or small containers/Aquaria <20 L; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Light; North Atlantic; Pelagos; Phytoplankton; Salinity; Single species; Species; Treatment; Generation; Partial pressure of carbon dioxide water at sea surface temperature wet air; Population; Replicates; Growth rate; Temperature, water; Alkalinity, total; Carbon, inorganic, dissolved; pH; Fugacity of carbon dioxide water at sea surface temperature wet air; Carbonate system computation flag; Carbon dioxide; Bicarbonate ion; Carbonate ion; Aragonite saturation state; Calcite saturation state; Potentiometric titration; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. 2010; Biological Impacts of Ocean Acidification BIOACID; Ocean Acidification International Coordination Centre OA-ICC; Ocean acidification
• Online Access: https://dx.doi.org/10.1594/pangaea.823153; https://doi.pangaea.de/10.1594/PANGAEA.823153

Predicting the impacts of environmental change on marine organisms, food webs, and biogeochemical cycles presently relies almost exclusively on short-term physiological studies, while the possibility of adaptive evolution is often ignored. Here, we assess adaptive evolution in the coccolithophore Emiliania huxleyi, a well-established model species in biological oceanography, in response to ocean acidification. We previously demonstrated that this globally important marine phytoplankton species adapts within 500 generations to elevated CO2. After 750 and 1000 generations, no further fitness increase occurred, and we observed phenotypic convergence between replicate populations. We then exposed adapted populations to two novel environments to investigate whether or not the underlying basis for high CO2-adaptation involves functional genetic divergence, assuming that different novel mutations become apparent via divergent pleiotropic effects. The novel environment "high light" did not reveal such genetic divergence whereas growth in a low-salinity environment revealed strong pleiotropic effects in high CO2 adapted populations, indicating divergent genetic bases for adaptation to high CO2. This suggests that pleiotropy plays an important role in adaptation of natural E. huxleyi populations to ocean acidification. Our study highlights the potential mutual benefits for oceanography and evolutionary biology of using ecologically important marine phytoplankton for microbial evolution experiments. : In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2013-11-28.